| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 894,963 | A→C | 14.6% | N253T (AAC→ACC) | potF → | putrescine transporter subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 894,963 | 0 | A | C | 14.6% | 84.8 / 19.4 | 48 | N253T (AAC→ACC) | potF | putrescine transporter subunit |
| Reads supporting (aligned to +/- strand): ref base A (6/35); new base C (3/4); total (9/39) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.11e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.62e-01 | |||||||||||
GGCAAACGGCGATATTTGCGTCGCTATCGGCTGGGCAGGTGATGTCTGGCAGGCGTCAAACCGCGCGAAGGAAGCGAAGAATGGCGTGAATGTCTCGTTCTCGATTCCAAAAGAAGGGGCGATGGCGT > W3110S.gb/894904‑895031 | ggCAAACGGCGATATTTGCGTCGCTATCGGCTGGGCAGGTGATGTCTGGCAGGCGTCAAACCGCGCgaa > 1:1271415/1‑69 (MQ=255) gCAAACGGCGATATTTGCGTCGCTATCGGCTGGGCAGGTGATGTCTGGCAGGCGTCAAACCGCGCgaagga < 1:1171014/71‑1 (MQ=255) gCAAACGGCGATATTTGCGTCGCTATCGGCTGGGCAGGTGATGTCTGGCAGGCGTCAAACCGCGCgaagg < 1:1384674/70‑1 (MQ=255) aaaCGGCGATATTTGCGTCGCTATCGGCTGGGCAGGTGATGTCTGGCAGGCGTCAACCCGCGCgaagg > 1:834882/1‑68 (MQ=255) gCGATATTTGCGTCGCTATCGGCTGGGCAGGTGATGTCTGGCAGGCGTCAAACCGCGCTAAGGAAGCgaag < 1:1763010/71‑1 (MQ=255) gCGATATTTGCGTCGCTATCGGCTGGGCAGGTGATGTCTGGCAGGCGTCAAACCGCGCGAAGGAAGCgaag < 1:1236420/71‑1 (MQ=255) gCGATATTTGCGTCGCTATCGGCTGGGCAGGTGATGTCTGGCAGGCGTCAAACCGCGCGAAGGAAGCgaag < 1:1564773/71‑1 (MQ=255) cGATATTTGCGTCGCTATCGGCTGGGCAGGTGATGTCTGGCAGGCGTCAAACCGCGCGAAGGAAGCgaag < 1:53679/70‑1 (MQ=255) ttGCGTCGCTATCGGCTGGGCAGGTGATGTCTGGCAGGCGTCAAACCGCGCGAAGGAAGCgaag < 1:428306/64‑1 (MQ=255) ttGCGTCGCTATCGGCTGGGCAGGTGATGTCTGGCAGGCGTCAAACCGCGCGAAGGAAGCGAAGAAt > 1:1552924/1‑67 (MQ=255) tGCGTCGCTATCGGCTGGGCAGGTGATGTCTGGCAGGCGTCAACCCGCGCGAAGGAAGCGAAGAATGGCGt > 1:1815023/1‑71 (MQ=255) cGTCGCTATCGGCTGGGCAGGTGATGTCTGGCAGGCGTCAAACCGCGCGAAGGAAGCGAAGAATGGCGTg < 1:1793098/70‑1 (MQ=255) cGTCGCTATCGGCTGGGCAGGTGATGTCTGGCAGGCGTCAAACCGCGCGAAGGAAGCGAAGAATGGCGTg < 1:2027968/70‑1 (MQ=255) cGTCGCTATCGGCTGGGCAGGTGATGTCTGGCAGGCGTCAAACCGCGCGAAGGAAGCGAAGAATGGCGTg < 1:2042243/70‑1 (MQ=255) tCGCTATCGGCTGGGCAGGTGATGTCTGGCAGGCGTCAAACCGCGCgaagg < 1:2481698/51‑1 (MQ=255) tCGCTATCGGCTGGGCAGGTGATGTCTGGCAGGCGTCAAACCGCGCGAAGGAAGCgaagaa < 1:255596/61‑1 (MQ=255) tCGCTATCGGCTGGGCAGGTGATGTCTGGCAGGCGTCAAACCGCGCGAAGGAAGCgaagaa < 1:2254899/61‑1 (MQ=255) tCGCTATCGGCTGGGCAGGTGATGTCTGGCAGGCGTCAAACCGCGCGAAGGAAGCgaagaa < 1:373153/61‑1 (MQ=255) gCTATCGGCTGGGCAGGTGATGTCTGGCAGGCGTCAAACCGCGCGAAGGAAGCGAAGAATGGCGTGAATGt > 1:1541924/1‑71 (MQ=255) cTATCGGCTGGGCAGGTGATGTCTGGCAGGCGTCAAACCGCGCGAAGGAAGCgaag < 1:2570239/56‑1 (MQ=255) tATCGGCTGGGCAGGTGATGTCTGGCAGGCGTCAAACCGCGCgaagg < 1:316852/47‑1 (MQ=255) tCGGCTGGGCAGGTGATGTCTGGCAGGCGTCAAACcgcgcg < 1:168728/41‑1 (MQ=255) tCGGCTGGGCAGGTGATGTCTGGCAGGCGTCAAACCGCGCGAAGGAAGCGAAGAATg < 1:1619812/57‑1 (MQ=255) tCGGCTGGGCAGGTGATGTCTGGCAGGCGTCAAACCGCGCGAAGGAAGCGAAGAATg < 1:429361/57‑1 (MQ=255) tCGGCTGGGCAGGTGATGTCTGGCAGGCGTCAAACCGCGCGAAGGAAGCGAAGAATg < 1:120835/57‑1 (MQ=255) tCGGCTGGGCAGGTGATGTCTGGCAGGCGTCAAACCGCGCGAAGGAAGCGAAGAATg < 1:1166844/57‑1 (MQ=255) ggCTGGGCAGGTGATGTCTGGCAGGCGTCAAACCGCGCGAAGGAAGCGAAGAATGGCGTGaa > 1:1127684/1‑62 (MQ=255) gCTGGGCAGGTGATGTCTGGCAGGCGTCAAACCGCGCGAAGGAAGCgaag < 1:2192436/50‑1 (MQ=255) ggCAGGTGATGTCTGGCAGGCGTCAAACCGCGCGAAGGAAGCGAAGAATGGCGTGAATGTCTCGTTCTCg < 1:1393486/70‑1 (MQ=255) gTGATGTCTGGCAGGCGTCAAACCGCGCGAAGGAAGCGAAGAATGGCGTGAATGTCTCGTtctc < 1:394647/64‑1 (MQ=255) tGATGTCTGGCAGGCGTCAACCCGCGCGAAGGAAGCGAAGAATGGCGTg < 1:213095/49‑1 (MQ=255) tGATGTCTGGCAGGCGTCAACCCGCGCGAAGGAAGCGAAGAATGGCGTg < 1:1145088/49‑1 (MQ=255) tGATGTCTGGCAGGCGTCAACCCGCGCGAAGGAAGCGAAGAATGGCGTg < 1:2768739/49‑1 (MQ=255) tGATGTCTGGCAGGCGTCAACCCGCGCGAAGGAAGCGAAGAATGGCGTg < 1:2684823/49‑1 (MQ=255) aTGTCTGGCAGGCGTCAAACCGCGCGAAGGAAGCGAAGAATGGCGTGAATGTCTCGTTCTCGATTCCaaaa < 1:1565920/71‑1 (MQ=255) tGTCTGGCAGGCGTCAAAGCGCGCGAAGGAATCGAAGAATGGCGTGAATGTCTCGTtc < 1:745226/58‑1 (MQ=255) tGTCTGGCAGGCGTCAAACCGCGCGAAGGAAGCgaa > 1:1553206/1‑36 (MQ=255) tGTCTGGCAGGCGTCAAACCGCGCGAAGGAAGCGAAGAATGGCGTGAATGTCTCGTtc < 1:1024611/58‑1 (MQ=255) tGTCTGGCAGGCGTCAAACCGCGCGAAGGAAGCGAAGAATGGCGTGAATGTCTCGTTCTCGATTCCAAaag < 1:798681/71‑1 (MQ=255) tGTCTGGCAGGCGTCAAACCGCGCGAAGGAAGCGAAGAATGGCGTGAATGTCTCGTTCTCGATTCCAAaag < 1:2479407/71‑1 (MQ=255) tGTCTGGCAGGCGTCAAACCGCGCGAAGGAAGCGAAGAATGGCGTGAATGTCTCGTTCTCGATTCCAAaag < 1:2674899/71‑1 (MQ=255) ggCGTCAAACCGCGCGAAGGAAGCGAAGAATGGCGTGAATGTCTCGTTCTCGATTCCAAAAGAAGGGGCGa < 1:12488/71‑1 (MQ=255) cGTCAACCCGCGCGAAGGAAGCGAAGAATGGCGTGAATGTCTCGTTCTCGATTCCAAAAGAAGGGGCGATg > 1:1804931/1‑71 (MQ=255) cGTCAAACCGCGCGAAGGATGCGAAGAATGGCGTGAATGTCTCGTtc < 1:1445048/47‑1 (MQ=255) gTCAAACCGCGCGAAGGAAGCGAAGAATGGCGTGAATGTCTCGTTCTCGATTCCAAaaga > 1:2559297/1‑60 (MQ=255) tCAAACCGCGCGAAGGAAGCGAAGAATGGCGTGAATGTCTCGTTCTCGa < 1:2561578/49‑1 (MQ=255) tCAAACCGCGCGAAGGAAGCGAAGAATGGCGTGAATGTCTCGTTCTCGATTCCAAaag < 1:1675975/58‑1 (MQ=255) tCAAACCGCGCGAAGGAAGCGAAGAATGGCGTGAATGTCTCGTTCTCGATTCCAAAAGAAGGGGc < 1:1450286/65‑1 (MQ=255) aaaCCGCGCGAAGGAAGCGAAGAATGGCGTGAATGTCTCGTTCTCGATTCCAAaagaa > 1:619232/1‑58 (MQ=255) aaaCCGCGCGAAGGAAGCGAAGAATGGCGTGAATGTCTCGTTCTCGATTCCAAaagaa > 1:156128/1‑58 (MQ=255) aaaCCGCGCGAAGGAAGCGAAGAATGGCGTGAATGTCTCGTTCTCGATTCCAAaagaa > 1:165391/1‑58 (MQ=255) aaaCCGCGCGAAGGAAGCGAAGAATGGCGTGAATGTCTCGTTCTCGATTCCAAAAGAAGGGGc < 1:2273967/63‑1 (MQ=255) aaaCCGCGCGAAGGAAGCGAAGAATGGCGTGAATGTCTCGTTCTCGATTCCAAAAGAAGGGGCGa > 1:861719/1‑65 (MQ=255) aaaCCGCGCGAAGGAAGCGAAGAATGGCGTGAATGTCTCGTTCTCGATTCCAAAAGAAGGGGCGATGGCGt > 1:2798573/1‑71 (MQ=255) aaaCCGCGCGAAGGAAGCGAAGAATGGCGTGAATGTCTCGTTCTCGATTCCAAAAGAAGGGGCGATGGCGt > 1:2736012/1‑71 (MQ=255) aaaCCGCGCGAAGGAAGCGAAGAATGGCGTGAATGTCTCGTTCTCGATTCCAAAAGAAGGGGCGATGGCGt > 1:1464649/1‑71 (MQ=255) aaaCCGCGCGAAGGAAGCGAAGAATGGCGTGAATGTCTCGTTCTCGATTCCAAAAGAAGGGGCGATGGCGt > 1:1524183/1‑71 (MQ=255) | GGCAAACGGCGATATTTGCGTCGCTATCGGCTGGGCAGGTGATGTCTGGCAGGCGTCAAACCGCGCGAAGGAAGCGAAGAATGGCGTGAATGTCTCGTTCTCGATTCCAAAAGAAGGGGCGATGGCGT > W3110S.gb/894904‑895031 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |