| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 940,557 | A→G | 78.0% | P236P (CCA→CCG) | serS → | seryl‑tRNA synthetase, also charges selenocysteinyl‑tRNA with serine |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 940,557 | 0 | A | G | 78.0% | 60.9 / 13.7 | 32 | P236P (CCA→CCG) | serS | seryl‑tRNA synthetase, also charges selenocysteinyl‑tRNA with serine |
| Reads supporting (aligned to +/- strand): ref base A (2/5); new base G (19/5); total (22/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.18e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.44e-01 | |||||||||||
CGATCTGTTCCATACTCGTCCGCTGGAAGAAGAAGCAGACACCAGTAACTATGCGCTGATCCCAACGGCAGAAGTTCCGCTGACTAACCTGGTGCGCGGTGAAATCATCGATGAAGATGATCTGCC > W3110S.gb/940494‑940619 | cGATCTGTTCCATACTCGTCCGCTGGAAGAAGAAGCAGACACCAGTAACTATGCGCTGATCCCGACGGCAg < 1:1470492/71‑1 (MQ=255) cTGTTCCATACTCGTCCGCTGGAAGAAGAAGCAGACACCAGTAACTATGCGCTGATCCCGACGGCAGAAGt > 1:2018149/1‑71 (MQ=255) ttCCATACTCGTCCGCTGGAAGAAGAAGCAGACACCAGTAACTATGCGCTGATCCCGACGGCAGAAGTTcc < 1:2150397/71‑1 (MQ=255) ccATACTCGTCCGCTGGAAGAAGAAGCAGACACCAGTAACTATGCGCTGATCCCGACGGCAGAAGTTCCGc > 1:1628221/1‑71 (MQ=255) ccATACTCGTCCGCTGGAAGAAGAAGCAGACACCAGTAACTATGCGCTGATCCCGACGGCAGAAGTTCAGc > 1:2606906/1‑71 (MQ=255) cATACTCGTCCGCTGGAAGAAGAAGCAGACACCAGTAACTATGCGCTGATCCCAACGGCAGAAGTTCCGCt < 1:1843902/71‑1 (MQ=255) cTCGTCCGCTGGAAGAAGAAGCAGACACCAGTAACTATGCGCTGATCCCGACGGCAGAAGTTCCGCTGAc > 1:77706/1‑70 (MQ=255) tCGTCCGCTGGAAGAAGAAGCAGACACCAGTAACTATGCGCTGATCCCAACGGCAGAAGTTCCGCTGACt > 1:941722/1‑70 (MQ=255) cGCTGGAAGAAGAAGCAGACACCAGTAACTATGCGCTGATCCCGACGGCAGAAGTTCCGCTGACTAACCTg < 1:718874/71‑1 (MQ=255) agaagCAGACACCAGTAACTATGCGCTGATCCCGACGGCAGAAGTTCCGCTGACTAACCTGGTGCGCGGt > 1:830043/1‑70 (MQ=255) gaagCAGACACCAGTAACTATGCGCTGATCCCGACGGCAGAAGTTCCGCTGACTAAc > 1:1080647/1‑57 (MQ=255) agCAGACACCAGTAACTATGCGCTGATCCCAACGGCAGAAGTTCCGCTGACTAACCTGGTGCGCGGTGaaa > 1:675122/1‑71 (MQ=255) acacCAGTAACTATGCGCTGATCCCAACGGCAGAAGTTCCGCTGACTAACCTGGTGCGCGGTGa < 1:995024/64‑1 (MQ=255) acacCAGTAACTATGCGCTGATCCCAACGGCAGAAGTTCCGCTGACTAACCTGGTGCGCGGTGa < 1:863501/64‑1 (MQ=255) acacCAGTAACTATGCGCTGATCCCAACGGCAGAAGTTCCGCTGACTAACCTGGTGCGCGGTGa < 1:1924683/64‑1 (MQ=255) acCAGTAACTATGCGCTGATCCCGACGGCAGAAGTTc > 1:2112116/1‑37 (MQ=255) acCAGTAACTATGCGCTGATCCCGACGGCAGAAGTTc > 1:34455/1‑37 (MQ=255) acCAGTAACTATGCGCTGATCCCGACGGCAGAAGTTc > 1:452212/1‑37 (MQ=255) ccAGTAACTATGCGCTGATCCCGACGGCAGAAGTTCCGCTGACTAACCTGGTGCTCGGTGaaa < 1:1243035/63‑1 (MQ=255) aGTAACTATGCGCTGATCCCGACGGCAGAAGTTCCGCTGACTAACCTGGTGCGCGGTGaa > 1:119661/1‑60 (MQ=255) aGTAACTATGCGCTGATCCCGACGGCAGAAGTTCCGCTGACTAACCTGGTGCGCGGTGAAatc > 1:2333513/1‑63 (MQ=255) aGTAACTATGCGCTGATCCCGACGGCAGAAGTTCCGCTGACTAACCTGGTGCGCGGTGAAATCATCGATg > 1:102639/1‑70 (MQ=255) aGTAACTATGCGCTGATCCCGACGGCAGAAGTTCCGCTGACTAACCTGGTGCGCGGTGAAATCATCGATg > 1:810721/1‑70 (MQ=255) gTAACTATGCGCTGATCCCGACGGCAGAAGTTCCGCTGACTAACCTGGAGCGCGGTGAAATCATCGATGaa < 1:413484/71‑1 (MQ=255) aCTATGCGCTGATCCCGACGGCAGAAGTTCCGCTGACTAACCt > 1:2092595/1‑43 (MQ=255) aCTATGCGCTGATCCCGACGGCAGAAGTTCCGCTGACTAACCTGGTGCGCGGTGAAATCATCGATGAAgat > 1:1476963/1‑71 (MQ=255) tATGCGCTGATCCCGACGGCAGAAGTTCCGCTGACTAAc > 1:785214/1‑39 (MQ=255) cgcTGATCCCTACGGCAGAAGTTCCGCTGACTAACCTGGTGCGCGGTGAAATCATCGATGAAGATGATCTg > 1:179723/1‑71 (MQ=255) cgcTGATCCCGACGGCAGAAGTTCCGCTGACTAACCTGGTGCGCGGTGAAat > 1:2210001/1‑52 (MQ=255) cgcTGATCCCGACGGCAGAAGTTCCGCTGACTAACCTGGTGCGCGGTGAAATCATCGATGAAGATGATCTg > 1:157955/1‑71 (MQ=255) tGATCCCGACGGCAGAAGTTCCGCTGACTAACCTGGTGCGCGGTGAAATCATCGATGAAGATGATCTGcc > 1:2179951/1‑70 (MQ=255) aTCCCAACGGCAGAAGTTCCGCTGACTAACCTGGTGCGCGGTGa < 1:1762252/44‑1 (MQ=255) | CGATCTGTTCCATACTCGTCCGCTGGAAGAAGAAGCAGACACCAGTAACTATGCGCTGATCCCAACGGCAGAAGTTCCGCTGACTAACCTGGTGCGCGGTGAAATCATCGATGAAGATGATCTGCC > W3110S.gb/940494‑940619 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |