Predicted mutation
evidence seq id position mutation freq annotation gene description
RA W3110S.gb 1,006,848 A→T 100% K159* (AAA→TAA)  ycbW → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb1,006,8480AT88.9% 64.8 / 2.4 27K159* (AAA→TAA) ycbWhypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (1/2);  new base T (10/14);  total (11/16)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.62e-01
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

ATGCCTGCTGGCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCAGTTGGGCGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTCT  >  W3110S.gb/1006784‑1006914
                                                                |                                                                  
aTGCCTGCTGGCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCAGTTGGGCGACGCCATTTAAATCa                                                              >  1:271229/1‑71 (MQ=255)
aTGCCTGCTGGCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCAGTTGGGCGACGCCATTAAAATCa                                                              >  1:522701/1‑71 (MQ=255)
 tGCCTGCTGGCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCAGTTGGGCGACGCCATTTAAATCAt                                                             >  1:1341158/1‑71 (MQ=255)
 tGCCTGCTGGCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCAGTTGGGCGACGCCATTTAAATCAt                                                             >  1:1918360/1‑71 (MQ=255)
   ccTGCTGGCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCAGTTGGGCGACGCCATTAAAATCATg                                                            <  1:105665/70‑1 (MQ=255)
       gtgGCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCAGTTGGGCGACGCCATTTAAATCATGAAc                                                         <  1:2634875/68‑1 (MQ=255)
       ctgGCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCAGTTGGGCGACGCCATTTAAATCATGAAc                                                         <  1:1903174/69‑1 (MQ=255)
               aCCCTGCGTTGTTATTGCGGGTCGCGCGATGCAGTTGGGCGACGCCATTTAAATCATGAACGACAGGCTg                                                >  1:2386463/1‑70 (MQ=255)
                 ccTGCGTTGTTATTGCGGGTCGCGCGATGCAGTTGGGCGACGCCATTaaa                                                                  <  1:777815/50‑1 (MQ=255)
                     cGTTGTTATTGCGGGTCGCGCGATGCAGTTGGGCGACGCCATTTAAATCATGaa                                                          >  1:758172/1‑54 (MQ=255)
                     cGTTGTTATTGCGGGTCGCGCGATGCAGTTGGGCGACGCCATTTAAATCATGaa                                                          >  1:2441019/1‑54 (MQ=255)
                      gttgttATTGCGGGTCGCGCGATGCAGTTGGGCGACGCCATTTAAATCATGAACGACAGGCTGAAACCGCa                                        <  1:1701249/71‑1 (MQ=255)
                       ttgttATTGCGGGTCGCGCGATGCAGTTGGGCGACGCCATTAAAATCATGAACGACAGGCTGAAACCg                                          <  1:2312822/68‑1 (MQ=255)
                         gttATTGCGGGTCGCGCGATGCAGTTGGGCGACGCCATTTAAATCa                                                              >  1:860069/1‑46 (MQ=255)
                         gttATTGCGGGTCGCGCGATGCAGTTGGGCGACGCCATTTAAATCATGAACGACAGGCTGAAACCGCAGGt                                     >  1:27893/1‑71 (MQ=255)
                          ttATTGCGGGTCGCGCGATGCAGTTGGGCGACGCCATTTAAATCATGAACGACAGGCTGAAACCGCAGGtt                                    <  1:1165481/71‑1 (MQ=255)
                             ttGCGGGTCGCGCGATGCAGTTGGGCGACGCCATTTAAATCATGAACGACAGGCTGAAACCGCAGGTTaa                                  <  1:1448065/70‑1 (MQ=255)
                             ttGCGGGTCGCGCGATGCAGTTGGGCGACGCCATTTAAATCATGAACGACAGGCTGAAACCGCAGGTTAAt                                 <  1:394343/71‑1 (MQ=255)
                                cGGGTCGCGCGATGCAGTTGGGCGACGCCATTTAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGtt                              <  1:1968346/71‑1 (MQ=255)
                                cGGGTCGCGCGATGCAGTTGGGCGACGCCATTTAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGtt                              <  1:1936308/71‑1 (MQ=255)
                                  ggTCGCGCGATGCAGTTGGGCGACGCCATTTAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGa                            >  1:1741188/1‑71 (MQ=255)
                                  ggTCGCGCGATGCAGTTGGGCGACGCCATTTAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGa                            >  1:1489010/1‑71 (MQ=255)
                                                gTTGGGCGACGCCATTTAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCg              <  1:2370182/71‑1 (MQ=255)
                                                gTTGGGCGACGCCATTTAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCg              <  1:151086/71‑1 (MQ=255)
                                                   gggCGACGCCATTTAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGaa            <  1:73679/70‑1 (MQ=255)
                                                            cATTTAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTCt  <  1:155257/71‑1 (MQ=255)
                                                            cATTTAAATCATGAACGAAAGGCTGAAACCGAAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTCt  <  1:960888/71‑1 (MQ=255)
                                                             aTTTAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGtt                              <  1:29499/42‑1 (MQ=255)
                                                                |                                                                  
ATGCCTGCTGGCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCAGTTGGGCGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTCT  >  W3110S.gb/1006784‑1006914

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: