| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,006,848 | A→T | 100% | K159* (AAA→TAA) | ycbW → | hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,006,848 | 0 | A | T | 88.9% | 64.8 / 2.4 | 27 | K159* (AAA→TAA) | ycbW | hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (1/2); new base T (10/14); total (11/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.62e-01 | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
ATGCCTGCTGGCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCAGTTGGGCGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTCT > W3110S.gb/1006784‑1006914 | aTGCCTGCTGGCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCAGTTGGGCGACGCCATTTAAATCa > 1:271229/1‑71 (MQ=255)aTGCCTGCTGGCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCAGTTGGGCGACGCCATTAAAATCa > 1:522701/1‑71 (MQ=255) tGCCTGCTGGCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCAGTTGGGCGACGCCATTTAAATCAt > 1:1341158/1‑71 (MQ=255) tGCCTGCTGGCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCAGTTGGGCGACGCCATTTAAATCAt > 1:1918360/1‑71 (MQ=255) ccTGCTGGCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCAGTTGGGCGACGCCATTAAAATCATg < 1:105665/70‑1 (MQ=255) gtgGCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCAGTTGGGCGACGCCATTTAAATCATGAAc < 1:2634875/68‑1 (MQ=255) ctgGCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCAGTTGGGCGACGCCATTTAAATCATGAAc < 1:1903174/69‑1 (MQ=255) aCCCTGCGTTGTTATTGCGGGTCGCGCGATGCAGTTGGGCGACGCCATTTAAATCATGAACGACAGGCTg > 1:2386463/1‑70 (MQ=255) ccTGCGTTGTTATTGCGGGTCGCGCGATGCAGTTGGGCGACGCCATTaaa < 1:777815/50‑1 (MQ=255) cGTTGTTATTGCGGGTCGCGCGATGCAGTTGGGCGACGCCATTTAAATCATGaa > 1:758172/1‑54 (MQ=255) cGTTGTTATTGCGGGTCGCGCGATGCAGTTGGGCGACGCCATTTAAATCATGaa > 1:2441019/1‑54 (MQ=255) gttgttATTGCGGGTCGCGCGATGCAGTTGGGCGACGCCATTTAAATCATGAACGACAGGCTGAAACCGCa < 1:1701249/71‑1 (MQ=255) ttgttATTGCGGGTCGCGCGATGCAGTTGGGCGACGCCATTAAAATCATGAACGACAGGCTGAAACCg < 1:2312822/68‑1 (MQ=255) gttATTGCGGGTCGCGCGATGCAGTTGGGCGACGCCATTTAAATCa > 1:860069/1‑46 (MQ=255) gttATTGCGGGTCGCGCGATGCAGTTGGGCGACGCCATTTAAATCATGAACGACAGGCTGAAACCGCAGGt > 1:27893/1‑71 (MQ=255) ttATTGCGGGTCGCGCGATGCAGTTGGGCGACGCCATTTAAATCATGAACGACAGGCTGAAACCGCAGGtt < 1:1165481/71‑1 (MQ=255) ttGCGGGTCGCGCGATGCAGTTGGGCGACGCCATTTAAATCATGAACGACAGGCTGAAACCGCAGGTTaa < 1:1448065/70‑1 (MQ=255) ttGCGGGTCGCGCGATGCAGTTGGGCGACGCCATTTAAATCATGAACGACAGGCTGAAACCGCAGGTTAAt < 1:394343/71‑1 (MQ=255) cGGGTCGCGCGATGCAGTTGGGCGACGCCATTTAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGtt < 1:1968346/71‑1 (MQ=255) cGGGTCGCGCGATGCAGTTGGGCGACGCCATTTAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGtt < 1:1936308/71‑1 (MQ=255) ggTCGCGCGATGCAGTTGGGCGACGCCATTTAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGa > 1:1741188/1‑71 (MQ=255) ggTCGCGCGATGCAGTTGGGCGACGCCATTTAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGa > 1:1489010/1‑71 (MQ=255) gTTGGGCGACGCCATTTAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCg < 1:2370182/71‑1 (MQ=255) gTTGGGCGACGCCATTTAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCg < 1:151086/71‑1 (MQ=255) gggCGACGCCATTTAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGaa < 1:73679/70‑1 (MQ=255) cATTTAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTCt < 1:155257/71‑1 (MQ=255) cATTTAAATCATGAACGAAAGGCTGAAACCGAAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTCt < 1:960888/71‑1 (MQ=255) aTTTAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGtt < 1:29499/42‑1 (MQ=255) | ATGCCTGCTGGCACAACCCTGCGTTGTTATTGCGGGTCGCGCGATGCAGTTGGGCGACGCCATTAAAATCATGAACGACAGGCTGAAACCGCAGGTTAATGTTGACAGCTTCAGCCTCGAACAGGCAGTCT > W3110S.gb/1006784‑1006914 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |