| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 121,347 | T→C | 70.0% | M69V (ATG→GTG) | aroP ← | aromatic amino acid transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 121,347 | 0 | T | C | 70.0% | 41.7 / 20.1 | 30 | M69V (ATG→GTG) | aroP | aromatic amino acid transporter |
| Reads supporting (aligned to +/- strand): ref base T (5/4); new base C (14/7); total (19/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.87e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.72e-01 | |||||||||||
GCAAAACTGCCCCAGTATTTATAAGCAAAGTGGCTAAAGGAGCCTGCGACAGGTTCTTCGACCACCATTTCACCCAGCTGACGCATGATCAGAAAGGCGATAAAACCAGCAATGGCGTAACCCAGGATAATC > W3110S.gb/121280‑121411 | gCAAAACTGCCCCAGTATTTATAAGCAAAGTGGCTAAAGGAGCCTGCGACAGGTTCTTCGACCACCATTTc < 1:2665663/71‑1 (MQ=255) aCTGCCCCAGTATTTATAAGCAAAGTGGCTAAAGGAGCCTGCGACAGGTTCTTCGACCACCACTTCAcc > 1:2604874/1‑69 (MQ=255) cTGCCCCAGTATTTATAAGCAAAGTGGCTAAAGGAGCCTGCGACAGGTTCTTCGACCACCACTTCACCCAg > 1:2232532/1‑71 (MQ=255) aTTTATAAGCAAAGTGGCTAAAGGAGCCTGCGACAGGTTCTTCGACCACCACTTCa > 1:121540/1‑56 (MQ=255) aTTTATAAGCAAAGTGGCTAAAGGAGCCTGCGACAGGTTCTTCGACCACCACTTCa > 1:145723/1‑56 (MQ=255) tttATAAGCAAAGTGGCTAAAGGAGCCTGCGACAGGTTCTTCGACCACCATTTCACCCAGCTGACGCATGa > 1:1613623/1‑71 (MQ=255) tttATAAGCAAAGTGGCTAAAGGAGCCTGCGACAGGTTCTTCGACCACCACTTCACCCAGCTGACGCATGa > 1:369186/1‑71 (MQ=255) tttATAAGCAAAGTGGCTAAAGGAGCCTGCGACAGGTTCTTCGACCACCACTTCACACAGCTGACGCATGa > 1:672758/1‑71 (MQ=255) tataAGCAAAGTGGCTAAAGGAGCCTGCGACAGGTTCTTCGACCACCATTTCAc > 1:1874059/1‑54 (MQ=255) tataAGCAAAGTGGCTAAAGGAGCCTGCGACAGGTTCTTCGACCACCACTTCACCCAGCTGACGCATGAt > 1:2568010/1‑70 (MQ=255) cTAAAGGAGCCTGCGACAGGTTCTTCGACCACCATTTCACCCAGCTGACGCATGATCAGAAAGGCGATaaa < 1:245965/71‑1 (MQ=255) cTAAAGGAGCCTGCGACAGGTTCTTCGACCACCACTTCACCCAGCTGACGCATGATCAGAAAGGCGATaaa < 1:785934/71‑1 (MQ=255) cTAAAGGAGCCTGCGACAGGTTCTTCGACCACCACTTCACCCAGCTGACGCATGATCAGAAAGGCGATaaa < 1:136289/71‑1 (MQ=255) aGGAGCCTGCGACAGGTTCTTCGACCACCATTTCACCCAGCTGACGCATGATCAGAAAGGCGATAAAAc > 1:671906/1‑69 (MQ=255) gAGCCTGCGACAGGTTCTTCGACCACCACTTCACCCAGCTGACGCATGATCAGAAAGGCGATAAAACCAGc < 1:809932/71‑1 (MQ=255) gAGCCTGCGACAGGTTCTTCGACCACCACTTCACACAGCTGACGCATGATCAGAAAGGCGATAAAACCAg > 1:1765003/1‑70 (MQ=255) aGCCTGCGACAGGTTCTTCGACCACCATTTCACCCAGCTGACGCATGATCAGAAAGGCGATAAAACCAGc > 1:2493861/1‑70 (MQ=255) gCCTGCGACAGGTTCTTCGACCACCACTTCACCCAGCTGACGCATGATCAGAAAGGCGATAAAACCAGCaa > 1:1483458/1‑71 (MQ=255) gCCTGCGACAGGTTCTTCGACCACCACTTCACCCAGCTGACGCATGATCAGAAAGGCGATAAAACCAGCaa > 1:2627602/1‑71 (MQ=255) gCCTGCGACAGGTTCTTCGACCACCACTTCACCCAGCTGACGCATGATCAGAAAGGCGATAAAACCAGCa > 1:116923/1‑70 (MQ=255) tGCGACAGGTTCTTCGACCACCATTTCACCCAGCTGACGCATGATCAGAAAGGCGATAAAACCAGCaa > 1:1399553/1‑68 (MQ=255) tGCGACAGGTTCTTCGACCACCACTTCACGCAGCTGACGCATGATCAGAAAGGCGATAAAACCAGCAATgg > 1:529240/1‑71 (MQ=255) tGCGACAGGTTCTTCGACCACCACTTCACCCAGCTGACGCATGATCAGAAAGGCGATAAAACCAGCAATgg > 1:1466209/1‑71 (MQ=255) aCAGGTTCTTCGACCACCACTTCACCCAGCTGACGCATGATCAGAAAGGCGATAAAACCAGc < 1:742622/62‑1 (MQ=255) aGGTTCTTCGACCACCACTTCACCCAGCTGACGCATGATCAGAAAGGCGATAAAACCAGCAATGGCGTAAc > 1:1500900/1‑71 (MQ=255) cttcGACCACCACTTCACCCAGCTGACGCATGATCAGAAAGGCGATAAAACCAGCAATGGCGTAACCCAg < 1:2304068/70‑1 (MQ=255) cGACCACCATTTCACCCAGCTGACGCATGATCAGAAAGGCGATAAAACCAGCAATTGCGTAACCCAGGAt < 1:2618157/70‑1 (MQ=255) cGACCACCACTTCACCCAGCTGACGCATGATCAGAAAGGCGATAAAACCAGCAATGGCGTAACCCAGGAt < 1:1583457/70‑1 (MQ=255) accaccATTTCACCCAGCTGACGCATGATCAGAAAGGCGATAAAACCAGCa < 1:603435/51‑1 (MQ=255) ccaccaCTTCACCCAGCTGACGCACGATCAGAAAGGCGATAAAACCAGCAATGGCGTAACCCAGGATAATc < 1:1021716/71‑1 (MQ=255) caccacTTCACCCAGCTGACGCATGATCAGAAAGGCGATaa < 1:2275620/41‑1 (MQ=255) caccacTTCACCCAGCTGACGCATGATCAGAAAGGCGATaa < 1:1544795/41‑1 (MQ=255) caccacTTCACCCAGCTGACGCATGATCAGAAAGGCGATAAAACCAGCaa > 1:384898/1‑50 (MQ=255) | GCAAAACTGCCCCAGTATTTATAAGCAAAGTGGCTAAAGGAGCCTGCGACAGGTTCTTCGACCACCATTTCACCCAGCTGACGCATGATCAGAAAGGCGATAAAACCAGCAATGGCGTAACCCAGGATAATC > W3110S.gb/121280‑121411 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |