| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,051,238 | T→C | 74.1% | intergenic (+286/+147) | insB → / ← cspH | IS1 transposase InsAB'/stress protein, member of the CspA‑family |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,051,238 | 0 | T | C | 74.1% | 45.4 / 13.9 | 27 | intergenic (+286/+147) | insB/cspH | IS1 transposase InsAB'/stress protein, member of the CspA‑family |
| Reads supporting (aligned to +/- strand): ref base T (5/2); new base C (7/13); total (12/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.85e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TTGTTACGAAAGCTATTAATTATTGTTGGTAATATAGTTTCAAGTGGTACTATAACTGGCTGTTAAATGGCATTTATAACTATTAGGTGCAGAGATATTCGCTTAAATGGATTAGTTTCTTTGTGGAA > W3110S.gb/1051175‑1051302 | ttGTTACGAAAGCTATTAATTATTGTTGGTAATATAGTTTCAAGTGGTACTATAACTGGCTGTCAAATGGc < 1:1344787/71‑1 (MQ=255) cGAAAGCTATTAATTATTGTTGGTAATATAGTTTCAAGTGGTACTATAACTGGCTGTCAAATGGCATTtat < 1:543226/71‑1 (MQ=255) cGAAAGCTATTAATTATTGTTGGTAATATAGTTTCAAGTGGTACTATAACTGGCTGTCAAATGGCATTtat < 1:1711098/71‑1 (MQ=255) cGAAAGCTATTAATTATTG‑TGGTAATATAGTTTCAAGTGGTACTATAACTGGCTGTCAAATGGCATTtat < 1:1046732/70‑1 (MQ=255) aaGCTATTAATTATTGTTGGTAATATAGTTTCAAGTGGTACTATAACTGGCTGTTAAATGGCATTTATAAc < 1:1417191/71‑1 (MQ=255) attaattaTTGTTGGTAATATAGTTTCAAGTGGTACTATAACTGGCTTTCAAATGGCATTTATAACTAt > 1:2157103/1‑69 (MQ=255) aattaTTGTTGGTAATATAGTTTCAAGTGGTACTATAACTGGCTGTCAAATGGCATTTATAACTATTAGGt > 1:2606504/1‑71 (MQ=255) ttattGTTGGTAATATAGTTTCAAGTGGTACTATAACTGGCTGTCAAATGGCATTTATAACTATTAGGTGc < 1:714523/71‑1 (MQ=255) ttattGTTGGTAATATAGTTTCAAGTGGTACTATAACTGGCTGTCAAATGGCATTTATAACTATTAGGTGc < 1:2277848/71‑1 (MQ=255) atatAGTTTCAAGTGGTACTATAACTGGCTGTCAAAt < 1:1947693/37‑1 (MQ=255) atatAGTTTCAAGTGGTACTATAACTGGCTGTCAAATGGCATTTATAACTATTAGGTGCAGAGATATTCGc > 1:2385202/1‑71 (MQ=255) tataGTTTCAAGTGGTACTATAACTGGCTGTCAAATGGCATTTATAAc < 1:443777/48‑1 (MQ=255) ataGTTTCAAGTGGTACTATAACTGGCTGTTAAATGGCATTTATAACTATTAGGTGCAGAGATATTCGCt > 1:591418/1‑70 (MQ=255) tttCAAGTGGTACTATAACTGGCTGTCAAATGGCAtt < 1:900021/37‑1 (MQ=255) tCAAGTGGTACTATAACTGGCTGTCAAATGGCATTTATAACTATTAGGTGCAGAGATATTCGCTTAAATg > 1:2614056/1‑70 (MQ=255) tCAAGTGGTACTATAACTGGCTGTCAAATGGCATTTATAACTATTAGGTGCAGAGATATTCGCTTAAATg > 1:1180582/1‑70 (MQ=255) tCAAGTGGTACTATAACTGGCTGTCAAATGGCATTTATAACTATTAGGTGCAGAGATATTCGCTTAAATg > 1:1057123/1‑70 (MQ=255) aaGTGGTACTATAACTGGCTGTTAAATGGCATTTATAACTATTAGGTGCAGAGATATTCGCTTa < 1:1302494/64‑1 (MQ=255) aaGTGGTACTATAACTGGCTGTCAAATGGCATTTATAACTATTAGGTGCAGAGATATTCGCTTAAATGGAt < 1:2461396/71‑1 (MQ=255) gTACTATAACTGGCTGTCAAATGGCATTTATAACTATTAGGTGCAGAGATATTCGCTTAAATGGATTAGt < 1:2399605/70‑1 (MQ=255) cTATAACTGGCTGTCAAATGGCATTTATAACTATTAGGTGCAGAGATATTCGCTTa < 1:2704600/56‑1 (MQ=255) ataACTGGCTGTCAAATGGCATTTATAACTATTAGGTGCAGAGata > 1:2224388/1‑46 (MQ=255) taACTGGCTGTTAAATGGCATTTATAACTATTAGGTGCAGAGATATTCGCTTaaa > 1:2166804/1‑55 (MQ=255) taACTGGCTGTTAAATGGCATTTATAACTATTAGGTGCAGAGATATTCGCTTaaa > 1:1923466/1‑55 (MQ=255) gCTGTTAAATGGCATTTATAACTATTAGGTGCAgaga > 1:1333243/1‑37 (MQ=255) gCTGTTAAATGGCATTTATAACTATTAGGTGCAgaga > 1:623665/1‑37 (MQ=255) gCTGTCAAATGGCATTTATAACTATTAGGTGCAGAGATATTCGCTTAAATGGATTAGTTTCTTTGTGGaa < 1:310844/70‑1 (MQ=255) | TTGTTACGAAAGCTATTAATTATTGTTGGTAATATAGTTTCAAGTGGTACTATAACTGGCTGTTAAATGGCATTTATAACTATTAGGTGCAGAGATATTCGCTTAAATGGATTAGTTTCTTTGTGGAA > W3110S.gb/1051175‑1051302 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |