| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,068,568 | T→A | 78.0% | G22G (GGT→GGA) | ymdF → | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,068,568 | 0 | T | A | 78.0% | 53.4 / 10.1 | 27 | G22G (GGT→GGA) | ymdF | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base T (3/3); new base A (5/16); total (8/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.19e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ATGGCAAACCATCGAGGCGGTTCCGGCAATTTTGCAGAAGACCGCGAAAGAGCATCAGAAGCAGGTAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTG > W3110S.gb/1068503‑1068626 | aTGGCAAACCATCGAGGCGGTTCCGGCAATTTTGCAGAAGACCGCGAAAGAGCATCAGAAGCAGGTaaaa > 1:756851/1‑70 (MQ=255)aTGGCAAACCATCGAGGCGGTTCCGGCAATTTTGCAGAAGACCGCGAAAGAGCATCAGAAGCAGGAaaaa > 1:1917523/1‑70 (MQ=255) tGGCAAACCATCGAGGCGGTTCCGGCAATTTTGCAGAAGACCGCGAAAGAGCATCAGAAGCAGGAaaaaaa < 1:2102792/71‑1 (MQ=255) tGGCAAACCATCGAGGCGGTTCCGGCAATTTTGC‑‑AAGACCGCGAAAGAGCATCAGAAGCAGGAAAAAAAgg < 1:1246321/71‑1 (MQ=255) gCAAACCATCGAGGCGGTTCCGGCAATTTTGCAGAAGACCGCGAAAGAGCATCAGAAGCAGGAAAAAAAgg > 1:465668/1‑71 (MQ=255) aTCGAGGCGGTTCCGGCAATTTTGCAGAAGACCGCGAAAGAGCATCAGAAGCAGGAAAAAAAGGTGGacag < 1:934004/71‑1 (MQ=255) gCGGTTCCGGCAATTTTGCAGAAGACCGCGAAAGAGCATCAGAAGCAGGAAAAAAAGGTGGacagcacagc < 1:2315518/71‑1 (MQ=255) tCCGGCAATTTTGCAGAAGACCGCGAAAGAGCATCAGAAGCAGGTAAAAAAGGTGGACAGCACAGCggggg > 1:2540071/1‑71 (MQ=255) tCCGGCAATTTTGCAGAAGACCGCGAAAGAGCATCAGAAGCAGGTAAAAAAGGTGGACAGCACAGCggggg > 1:931914/1‑71 (MQ=255) tCCGGCAATTTTGCAGAAGACCGCGAAAGAGCATCAGAAGCAGGAAAAAAAg > 1:428369/1‑52 (MQ=255) ccGGCAATTTTGCAGAAGACCGCGAAAGAGCATCAGAAGCAGGAaaaa < 1:1451959/48‑1 (MQ=255) ggCAATTTTGCAGAAGACCGCGAAAGAGCATCAGAAGCAGGAAAAAAAGGTGGacag < 1:1545136/57‑1 (MQ=255) gCAATTTTGCAGAAGACCGCGAAAGAGCATCAGAAGCAGGAaaaa < 1:1490229/45‑1 (MQ=255) gCAATTTTGCAGAAGACCGCGAAAGAGCATCAGAAGCAGGAAAAAAAGGTGGACAGCACAGCGGGGGTAAt > 1:896796/1‑71 (MQ=255) ttttGCAGAAGACCGCGAAAGAGCATCAGAAGCAGGAAAAAAAGGTGGacagcac < 1:695422/55‑1 (MQ=255) ttttGCAGAAGACCGCGAAAGAGCATCAGAAGCA‑GTaaaaa < 1:967493/41‑1 (MQ=38) tGCAGAAGACCGCGAAAGAGCATCAGAAGCAGGTAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAg < 1:1302307/70‑1 (MQ=255) tGCAGAAGACCGCGAAAGAGCATCAGAAGCAGGAAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAg < 1:1117081/70‑1 (MQ=255) cAGAAGACCGCGAAAGAGCATCAGAAGCAGGAAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGatt < 1:663540/71‑3 (MQ=255) cAGAAGACCGCGAAAGAGCATCAGAAGCAGGAAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAAt < 1:460794/71‑1 (MQ=255) gaagaCCGCGAAAGAGCATCAGAAGCAGGAAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGa < 1:2129463/71‑1 (MQ=255) tCAGAAGCAGGAAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAg < 1:427889/48‑1 (MQ=255) tCAGAAGCAGGAAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGaa < 1:2566478/50‑1 (MQ=255) tCAGAAGCAGGAAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGaa < 1:1161177/50‑1 (MQ=255) tCAGAAGCAGGAAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCa < 1:2724279/66‑1 (MQ=255) tCAGAAGCAGGAAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTg > 1:500979/1‑70 (MQ=255) cAGGTAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAgcgcg < 1:1616121/57‑1 (MQ=255) | ATGGCAAACCATCGAGGCGGTTCCGGCAATTTTGCAGAAGACCGCGAAAGAGCATCAGAAGCAGGTAAAAAAGGTGGACAGCACAGCGGGGGTAATTTCAAGAATGACCCGCAGCGCGCATCTG > W3110S.gb/1068503‑1068626 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |