| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,110,226 | G→T | 15.1% | A98D (GCC→GAC) | mdoC ← | membrane protein required for modification of periplasmic glucan |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,110,226 | 0 | G | T | 15.1% | 93.8 / 4.1 | 33 | A98D (GCC→GAC) | mdoC | membrane protein required for modification of periplasmic glucan |
| Reads supporting (aligned to +/- strand): ref base G (20/8); new base T (3/2); total (23/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.27e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CAACTTTCCGCTTTTCCTTTGACATATTGCAGCATAATAAATTGCGGTAATGTCAGTAGGGGGATGGCTGTTAACATCGGGATACCTACACGTTCGACACGTACTTTCCACCATTTTTTCAAGGGATAGCGTAAAA > W3110S.gb/1110160‑1110295 | cAACTTTCCGCTTTTCCTTTGACATATTGCAGCATAATAAATTGCGGTAATGTCAGTAGGGGGATGTCTGt > 1:2213428/1‑71 (MQ=255) aaCTTTCCGCTTTTCCTTTGACATATTGCAGCATAATAAATTGCGGTAATGTCAGTAGGGGGATGGCTGt > 1:2292635/1‑70 (MQ=255) tCCGCTTTTCCTTTGACATATTGCAGCATAATAAATTGCGGTAATGTCAGTAGGGGGATGTCTGATAACAt > 1:2234932/1‑71 (MQ=255) tGACATATTGCAGCATAATAAATTGCGGTAATGTCAGTAGGGGGATGGCTGTTAACATCGGGATACCTaca > 1:1770178/1‑71 (MQ=255) tGACATATTGCAGCATAATAAATTGCGGTAATGTCAGTAGGGGGATGGCTGTTAACATCGGGATACCTa < 1:1646966/69‑1 (MQ=255) tatTGCAGCATAATAAATTGCGGTAATGTCAGTAGGGGGATGTCTGTTAACATCgg < 1:575587/56‑1 (MQ=255) tatTGCAGCATAATAAATTGCGGTAATGTCAGTAGGGGGATGTCTGTTAACATCgg < 1:2309352/56‑1 (MQ=255) tatTGCAGCATAATAAATTGCGGTAATGTCAGTAGGGGGATGGCTGTTAACATCGGGATACCTACACGTTc < 1:1329214/71‑1 (MQ=255) tatTGCAGCATAATAAATTGCGGTAATGTCAGTAGGGGGATGGCTGTTAACATCGGGATACCTACACGTTc > 1:1741327/1‑71 (MQ=255) ttGCAGCATAATAAATTGCGGTAATGTCAGTAGGGGGATGGCTGtt > 1:1726864/1‑46 (MQ=255) ttGCAGCATAATAAATTGCGGTAATGTCAGTAGGGGGATGGCTGTTAACATCGGGATACCTACACGTTCGa > 1:1207692/1‑71 (MQ=255) ttGCAGCATAATAAATTGCGGTAATGTCAGTAGGGGGATGGCTGTTAACATCGGGATACCTACACGTTCGa > 1:2573694/1‑71 (MQ=255) ttGCAGCATAATAAAATGCGGTAATGTCAGTAGGGGGATGGCTGTTAACATCGGGATACCTACACGTTCGa > 1:462056/1‑71 (MQ=255) gcagcaTAATAAATTGCGGTAATGTCAGTAGGGGGATGGCTGTTaa > 1:941603/1‑46 (MQ=255) gcagcaTAATAAATTGCGGTAATGTCAGTAGGGGGATGGCTGTTaa > 1:1421136/1‑46 (MQ=255) agcaTAATAAATTGCGGTAATGTCAGTAGGGGGATGGCTGTTAAc < 1:683154/45‑1 (MQ=255) agcaTAATAAATTGCGGTAATGTCAGTAGGGGGATGGCTGTTAACATCGGGATACCTACACGTTCGACACg < 1:1957946/71‑1 (MQ=255) taataaATTGCGGTAATGTCAGTAGGGGGATGTCTGTTAACATCGGGATACCTACACGTTCGACACg > 1:1083440/1‑67 (MQ=255) taataaATTGCGGTAATGTCAGTAGGGGGATGGCTGTTAACATCGGGATACCTACACGTTCGACACGTACt > 1:2300848/1‑71 (MQ=255) aataaATTGCGGTAATGTCAGTAGGGGGATGGCTGTTAACATCGGGATACCTACACGTTCGACACGTACtt > 1:2299411/1‑71 (MQ=255) ataaATTGCGGTAATGTCAGTAGGGGGATGGCTGTTAACATCGGGATACCTACACGTTCGa > 1:150450/1‑61 (MQ=255) ataaATTGCGGTAATGTCAGTAGGGGGATGGCTGTTAACATCGGGATACCTACACGTTCGACACGTACttt > 1:1346043/1‑71 (MQ=255) tGCGGTAATGTCAGTAGGGGGATGGCTGTTAACATCGGGATACCTACACGTTCGACACGTACTTTccacca > 1:306784/1‑71 (MQ=255) gCGGTAATGTCAGTAGGGGGATGGCTGTTAACATCGGGATACCTACACGTTCGACACGTa > 1:1221059/1‑60 (MQ=255) gCGGTAATGTCAGTAGGGGGATGGCTGTTAACATCGGGATACCTACACGTTCGACACGTACTTTCCACCAt > 1:1409448/1‑71 (MQ=255) ggTAATGTCAGTAGGGGGATGGCTGTTAACATCGGGATACCTACACGTTCGACACGTACTTTCCACCAtt < 1:1383338/70‑1 (MQ=255) tGTCAGTAGGGGGATGGCTGTTAACATCGGGATACCTa < 1:1741031/38‑1 (MQ=255) tCAGTAGGGGGATGGCTGTTAACATCGGGATACCTACACGTTCGact > 1:1773740/1‑46 (MQ=255) tCAGTAGGGGGATGGCTGTTAACATCGGGATACCTACACGTTCGACACGTACTTTCCACCATTTTTTCAAg > 1:1473694/1‑71 (MQ=255) tAGGGGGATGGCTGTTAACATCGGGATACCTACACGTTCGACACGTACTTTCCACCATTTTTTCAAg < 1:1527606/67‑1 (MQ=255) aGGGGGATGGCTGTTAACATCGGGATACCTACACGTTCGACACGTACtt > 1:1018786/1‑49 (MQ=255) gggggATGGCTGTTAACATCGGGATACCTACACGTTCGACACGTACTTTCCACCATTTTTTCaa > 1:522831/1‑64 (MQ=255) gATGGCTGTTAACATCGGGATACCTACACGTTCGACACGTACTTTCCACCATTTTTTc < 1:143035/58‑1 (MQ=255) gCTGTTAACATCGGGATACCTACACGTTCGACACGTACTTTCCACCATTTTTTCAAGGGATAGCGTaaaa > 1:80615/1‑70 (MQ=255) | CAACTTTCCGCTTTTCCTTTGACATATTGCAGCATAATAAATTGCGGTAATGTCAGTAGGGGGATGGCTGTTAACATCGGGATACCTACACGTTCGACACGTACTTTCCACCATTTTTTCAAGGGATAGCGTAAAA > W3110S.gb/1110160‑1110295 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |