Predicted mutation | |||||||
---|---|---|---|---|---|---|---|
evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | W3110S.gb | 1,150,273 | A→G | 100% | intergenic (+5/‑63) | plsX → / → fabH | fatty acid/phospholipid synthesis protein/3‑oxoacyl‑[acyl‑carrier‑protein] synthase III |
Read alignment evidence... | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | W3110S.gb | 1,150,273 | 0 | A | G | 84.2% | 40.8 / 2.2 | 19 | intergenic (+5/‑63) | plsX/fabH | fatty acid/phospholipid synthesis protein/3‑oxoacyl‑[acyl‑carrier‑protein] synthase III |
Reads supporting (aligned to +/- strand): ref base A (1/2); new base G (7/9); total (8/11) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.80e-01 | |||||||||||
Rejected as polymorphism: Variant not supported by required number of reads on each strand. |
ATCTGTATACCCAGCTGGTTTTGAGCTGCTGGACGGTGGCAAAAGCGGAACTCTGCGGTAGCAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACTGAGCGTACATGTA > W3110S.gb/1150208‑1150340 | aTCTGTATACCCAGCTGGTTTTGAGCTGCTGGACGGTGGCAAAAGCGGAACTCTGCGGTAGCAGGGCGCTg > 1:1133604/1‑71 (MQ=255) ggTTTTGAGCTGCTGGACGGTGGCAAAAGCGGAACTCTGCGGTAGCAGGGCGCTGCCAGCGAACTGGCAGt > 1:2304263/1‑71 (MQ=255) gTTTTGAGCTGCTGGACGGTGGCAAAAGCGGAACTCTGCGGTAGCAGGGCGCTGCCAGAGAACTCGCAGtt > 1:638130/1‑71 (MQ=255) gTTTTGAGCTGCTGGACGGTGGCAAAAGCGGAACTCTGCGGTAGCAGGACGCTGCCAGCGAACTCGCAg < 1:2477224/69‑1 (MQ=255) ctgctgGACGGTGGCAAAAGCGGAACTCTGCGGTAGCAGGGCGCTGCCAGCGAACTCGCAGTTTGCAAGt > 1:271259/1‑70 (MQ=255) gACGGTGGCAAAAGCGGAACTCTGCGGTAGCAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACgg < 1:309490/69‑1 (MQ=255) aaaGCGGAACTCTGCGGTAGCAGGGCGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGaa < 1:1590705/71‑1 (MQ=255) aaaGCGGAACTCTGCGGTAGCAGGGCGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGaa < 1:796212/71‑1 (MQ=255) ctGCGGTAGCAGGGCGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACTGa > 1:1712971/1‑70 (MQ=255) gCGGTAGCAGGGCGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTg < 1:2260107/63‑1 (MQ=255) cGGTAGCAGGGCGCTGCCAGCGAACTCGCAGTTTGCAAGTGACTGTATATAACCGAAAAGTGACTTAGCg < 1:2593523/70‑1 (MQ=255) cGGTAGCAGGGCGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACTGAg < 1:2603312/68‑1 (MQ=255) gTAGCAGGGCGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATaa < 1:1327688/50‑1 (MQ=255) gTAGCAGGGCGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACTGAGCGTAc < 1:2093050/71‑1 (MQ=255) gTAGCAGGGCGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACTGAGCGTAc < 1:184902/71‑1 (MQ=255) tAGCAGGGCGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGtatata < 1:1666851/48‑1 (MQ=255) aGCAGGGCGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACTGAGCGTACAt > 1:1865685/1‑71 (MQ=255) aGCAGGGCGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACTGAGCGTACAt > 1:586043/1‑71 (MQ=255) aGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACTGAGCGTACATGta > 1:904155/1‑71 (MQ=255) | ATCTGTATACCCAGCTGGTTTTGAGCTGCTGGACGGTGGCAAAAGCGGAACTCTGCGGTAGCAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACTGAGCGTACATGTA > W3110S.gb/1150208‑1150340 |
Alignment Legend |
---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |