Predicted mutation
evidence seq id position mutation freq annotation gene description
RA W3110S.gb 1,150,273 A→G 100% intergenic (+5/‑63) plsX → / → fabH fatty acid/phospholipid synthesis protein/3‑oxoacyl‑[acyl‑carrier‑protein] synthase III

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb1,150,2730AG84.2% 40.8 / 2.2 19intergenic (+5/‑63)plsX/fabHfatty acid/phospholipid synthesis protein/3‑oxoacyl‑[acyl‑carrier‑protein] synthase III
Reads supporting (aligned to +/- strand):  ref base A (1/2);  new base G (7/9);  total (8/11)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.80e-01
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

ATCTGTATACCCAGCTGGTTTTGAGCTGCTGGACGGTGGCAAAAGCGGAACTCTGCGGTAGCAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACTGAGCGTACATGTA  >  W3110S.gb/1150208‑1150340
                                                                 |                                                                   
aTCTGTATACCCAGCTGGTTTTGAGCTGCTGGACGGTGGCAAAAGCGGAACTCTGCGGTAGCAGGGCGCTg                                                                >  1:1133604/1‑71 (MQ=255)
                ggTTTTGAGCTGCTGGACGGTGGCAAAAGCGGAACTCTGCGGTAGCAGGGCGCTGCCAGCGAACTGGCAGt                                                >  1:2304263/1‑71 (MQ=255)
                 gTTTTGAGCTGCTGGACGGTGGCAAAAGCGGAACTCTGCGGTAGCAGGGCGCTGCCAGAGAACTCGCAGtt                                               >  1:638130/1‑71 (MQ=255)
                 gTTTTGAGCTGCTGGACGGTGGCAAAAGCGGAACTCTGCGGTAGCAGGACGCTGCCAGCGAACTCGCAg                                                 <  1:2477224/69‑1 (MQ=255)
                         ctgctgGACGGTGGCAAAAGCGGAACTCTGCGGTAGCAGGGCGCTGCCAGCGAACTCGCAGTTTGCAAGt                                        >  1:271259/1‑70 (MQ=255)
                               gACGGTGGCAAAAGCGGAACTCTGCGGTAGCAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACgg                                   <  1:309490/69‑1 (MQ=255)
                                         aaaGCGGAACTCTGCGGTAGCAGGGCGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGaa                       <  1:1590705/71‑1 (MQ=255)
                                         aaaGCGGAACTCTGCGGTAGCAGGGCGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGaa                       <  1:796212/71‑1 (MQ=255)
                                                    ctGCGGTAGCAGGGCGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACTGa             >  1:1712971/1‑70 (MQ=255)
                                                      gCGGTAGCAGGGCGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTg                  <  1:2260107/63‑1 (MQ=255)
                                                       cGGTAGCAGGGCGCTGCCAGCGAACTCGCAGTTTGCAAGTGACTGTATATAACCGAAAAGTGACTTAGCg          <  1:2593523/70‑1 (MQ=255)
                                                       cGGTAGCAGGGCGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACTGAg            <  1:2603312/68‑1 (MQ=255)
                                                         gTAGCAGGGCGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATaa                            <  1:1327688/50‑1 (MQ=255)
                                                         gTAGCAGGGCGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACTGAGCGTAc       <  1:2093050/71‑1 (MQ=255)
                                                         gTAGCAGGGCGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACTGAGCGTAc       <  1:184902/71‑1 (MQ=255)
                                                          tAGCAGGGCGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGtatata                             <  1:1666851/48‑1 (MQ=255)
                                                           aGCAGGGCGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACTGAGCGTACAt     >  1:1865685/1‑71 (MQ=255)
                                                           aGCAGGGCGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACTGAGCGTACAt     >  1:586043/1‑71 (MQ=255)
                                                              aGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACTGAGCGTACATGta  >  1:904155/1‑71 (MQ=255)
                                                                 |                                                                   
ATCTGTATACCCAGCTGGTTTTGAGCTGCTGGACGGTGGCAAAAGCGGAACTCTGCGGTAGCAGGACGCTGCCAGCGAACTCGCAGTTTGCAAGTGACGGTATATAACCGAAAAGTGACTGAGCGTACATGTA  >  W3110S.gb/1150208‑1150340

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: