| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,189,339 | A→G | 100% | L160S (TTA→TCA) | ycfD ← | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,189,339 | 0 | A | G | 95.7% | 69.4 / ‑3.8 | 23 | L160S (TTA→TCA) | ycfD | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (1/0); new base G (15/7); total (16/7) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.39e-01 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
AAGAATATCACCAGGCTCCAGCTCTTCATCGATGATGGCTTCGAACGGATCGACCTGTAACAGATCCGGATGTGGGCAGTGCTGTTTCATTTGCAGCTTTTCGCCCACTCGCCAGCGACGACGTCC > W3110S.gb/1189281‑1189406 | aaGAATATCACCAGGCTCCAGCTCTTCATCGATGATGGCTTCGAACGGATCGACCTGTGACAGATCCGGAt > 1:1845622/1‑71 (MQ=255) cAGGCTCCAGCTCTTCATCGATGATGGCTACGAACGGATCGACCTGTGACAGATCCGGATGTGGGCAGTGc < 1:1389224/71‑1 (MQ=255) tCCAGCTCTTCATCGATGATGGCTTCGAACGGATCGACCTGTGACAGATCCGGATGTGGGc < 1:701288/61‑1 (MQ=255) tCCAGCTCTTCATCGATGATGGCTTCGAACGGATCGACCTGTGACAGATCCGGATGTGGGCAGTGCTGttt > 1:2489062/1‑71 (MQ=255) ccAGCTCTTCATCGATGATGGCTTCGAACGGATCGACCTGTGACAGATCCGGa > 1:946681/1‑53 (MQ=255) ccAGCTCTTCATCGATGATGGCTTCGAACGGATCGACCTGTGACAGATCCGGa > 1:2385823/1‑53 (MQ=255) ctctTCATCGATGATGGCTTCGAACGGATCGACCTGTGACAGATCCGGATGTGGGCAGTGCTGTTTc < 1:2185872/67‑1 (MQ=255) cATCGATGATGGCTTCGAACGGATCGACCTGTGACAGATCCGGATGTGGGCAGTGCTGTTTCATTTGCAGc > 1:2273910/1‑71 (MQ=255) cATCGATGATGGCTTCGAACGGATCGACCTGTGACAGATCCGGATGTGGGCAGTGCTGTTTCACTTGCAGc > 1:2554057/1‑71 (MQ=255) tCGATGATGGCTTCGAACGGATCGACCTGTGACAGATCCGGa < 1:2317929/42‑1 (MQ=255) gatgatGGCTTCGAACGGATCGCCCTGTGACAGATCCGGATGTGGGCAGTGCTGTTTCATTTGCAGCtttt > 1:2662597/1‑71 (MQ=255) gatgatGGCTTCGAACGGATCGACCTGTGACAGATCCGGATGTGGGCAGTGCTGTTTCATTTGCAGCttt > 1:714349/1‑70 (MQ=255) atgatgGCTTCGAACGGATCGACCTGTGACAGATCCGGa < 1:1649049/39‑1 (MQ=255) atgGCTTCGAACGGATCGACCTGTGACAGATCCGGAtgt > 1:1119540/1‑39 (MQ=255) atgGCTTCGAACGGATCGACCTGTGACAGATCCGGATGTGGGCAGTGCTGTTTCATTTGCAGCTTTTCGc > 1:1369685/1‑70 (MQ=255) ggCTTCGAACGGATCGACCTGTGACAGATCCGGATGTGGGCAGTGCTGTTTCAt > 1:629813/1‑54 (MQ=255) aaCGGATCGACCTGTGACAGATCCGGATGTGGGCAGTGCTGTTTCATTTGCAGCTTTTc < 1:2775070/59‑1 (MQ=255) ggATCGACCTGTGACAGATCCGGATGTGGGCAGTGCTGTTTCAt > 1:522393/1‑44 (MQ=255) ggATCGACCTGTGACAGATCCGGATGTGGGCAGTGCTGTTTCAt > 1:62011/1‑44 (MQ=255) ggATCGACCTGTGACAGATCCGGATGTGGGCAGTGCTGTTTCAt > 1:979737/1‑44 (MQ=255) cGACCTGTGACAGATCCGGATGTGGGCAGTGCTGTTTCATTTGCAGCTCTTCGCCCACTCGCCAGcgacga > 1:2139846/1‑71 (MQ=255) cTGTGACAGATCCGGATGTGGGCAGTGCTGTTTCATTTGCAGCTTTTCGCCCACTCGCCAGc < 1:291168/62‑1 (MQ=255) tGTAACAGATCCGGATGTGGGCAGTGCTGTTTCATTTGCAGCTTTTCGCCCACTCGCCAGCGACGACGTcc > 1:548149/1‑71 (MQ=255) | AAGAATATCACCAGGCTCCAGCTCTTCATCGATGATGGCTTCGAACGGATCGACCTGTAACAGATCCGGATGTGGGCAGTGCTGTTTCATTTGCAGCTTTTCGCCCACTCGCCAGCGACGACGTCC > W3110S.gb/1189281‑1189406 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |