| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,197,638 | G→A | 67.6% | L313L (CTG→CTA) | icd → | isocitrate dehydrogenase, specific for NADP+ |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,197,638 | 0 | G | A | 67.6% | 26.1 / 19.5 | 26 | L313L (CTG→CTA) | icd | isocitrate dehydrogenase, specific for NADP+ |
| Reads supporting (aligned to +/- strand): ref base G (2/6); new base A (8/9); total (11/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.02e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.60e-01 | |||||||||||
CGGCTGAATATGATGTTATCGCCTGTATGAACCTGAACGGTGACTACATTTCTGACGCCCTGGCAGCGCAGGTTGGCGGTATCGGTATCGCCCCTGGTGCAAACATCGGTGACGAAT > W3110S.gb/1197577‑1197693 | cGGCTGAATATGATGTTATCGCCTGTATGAACCTGAACGGTGACTACATTTCTGACGCCCTAGCAGCGCa < 1:88543/70‑1 (MQ=255) tgatgTTATCGCCTGTATGAACCTGAACGGTGACTACATTTCTGACGCCCTAGCAGCGCAGGTTGGcggta > 1:665354/1‑71 (MQ=255) atgTTATCGCCTGTATGAACCTGAACGGTGACTACATTTCTGACGCCCTAGCAGCGCAGGTTGGcggtatc > 1:1676332/1‑71 (MQ=255) ttATCGCCTGTATGAACCTGAACGGTGACTACATTTCTGACGCCCTAGCAGCGCAGGTTGGcggtatcggt < 1:2130338/71‑1 (MQ=255) ttATCGCCTGTATGAACCTGAACGGTGACTACATTTCTGACGCCCTAGCAGCGCAGGTTGGcggtatcgg < 1:1925538/70‑1 (MQ=255) gCCTGTATGAACCTGAACGGTGACTACATTTCTGACGCCCTAGCAGCGCAGGTTGGCGGTATCGGTATCGc < 1:454139/71‑1 (MQ=255) gCCTGTATGAACCTGAACGGTGACTACATTTCTGACGCCCTAGCAGCGCAGGTTGGCGGTATCGGTATCGc < 1:1774324/71‑1 (MQ=255) tGTATGAACCTGAACGGTGACTACATTTCTGACGCCCTTGCAg > 1:173478/1‑43 (MQ=255) tATGAACCTGAACGGTGACTACATTTCTGCCGCCCTAGCAGCGCAGGTTGGCGGTATCGGTATCGCCCCt > 1:1233953/1‑70 (MQ=255) tGAACCTGAACGGTGACTACATTTCTGACGCCCTAGCAGCGCa > 1:2252544/1‑43 (MQ=255) tGAACCTGAACGGTGACTACATTTCTGACGCCCTAGCAGCGCAGGTTGGCGGTATCGGTATCGCCCCTGGt > 1:710892/1‑71 (MQ=255) aaCCTGAACGGTGACTACATTTCTGACGCCCTAGCAGCGCAGGTTGGCGGTATCGGTATCGCCCCTGGTg > 1:948058/1‑70 (MQ=255) aCCTGAACGGTGACTACATTTCTGACGCCCTGGCAGCGCAGGTTGGCGGTATCGGTATCGc < 1:2606949/61‑1 (MQ=255) aCCTGAACGGTGACTACATTTCTGACGCCCTGGCAGCGCAGGTT‑GCGGTATCGGTATCGc < 1:2399764/60‑1 (MQ=255) ggTGACTACATTTCTGACGCCCTGGCAGCGCAGGTTGGcggtat > 1:118670/1‑44 (MQ=255) gACTACATTTCTGACGCCCTAGCAGCGCAGGTTGGCGGTATCGGTATCGCCCCTGGTGCAAACATCgg > 1:1095185/1‑68 (MQ=255) cTACATTTCTGACGCCCTAGCAGCGCAGGTTGGCGGTATCGGTATCGCCCCTGGTGCAAACATCGGTGACg < 1:1544962/71‑1 (MQ=255) cTACATTTCTGACGCCCTAGCAGCGCAGGTTGGCGGTATCGGTATCGCCCCTGGTGCAAACATCGGTGACg < 1:1204785/71‑1 (MQ=255) cATTTCTGACGCCCTGGCAGCGCAGGTTGGCGGTATCGGTATCGCCCCTGGTGCAAACATCGGTGACg < 1:1957039/68‑1 (MQ=255) cATTTCTGACGCCCTGGCAGCGCAGGTTGGCGGTATCGGTATCGCCCCTGGTGCAAACATCGGTGACGAAt > 1:2221478/1‑71 (MQ=255) cATTTCTGACGCCCTAGCAGCGCAGGTTGGCGGTATCGGTATCGCCCCTGGTGCAAACATCGGTGACGaa < 1:37179/70‑1 (MQ=255) cATTTCTGACGCCCTAGCAGCGCAGGTTGGCGGTATCGGTATCGCCCCTGGTGCAAACATCGGTGACGa < 1:926149/69‑1 (MQ=255) aTTTCTGACGCCCTGGCAGCGCAGGTTGGCGGTATCGGTATCGCCCCTGGTGCAAACATCGGTGACg < 1:1775364/67‑1 (MQ=255) ttCTGACGCCCTAGCAGCGCAGGTTGGcggtatcggtat > 1:1535459/1‑39 (MQ=255) aCGCCCTGGCAGCGCAGGTTGGCGGTATCGTTATCGCCCCTGGTGCaaa < 1:1395673/49‑1 (MQ=255) aCGCCCTGGCAGCGCAGGTTGGCGGTATCGGTATCGCCCCTGGTGCaaa < 1:1216675/49‑1 (MQ=255) | CGGCTGAATATGATGTTATCGCCTGTATGAACCTGAACGGTGACTACATTTCTGACGCCCTGGCAGCGCAGGTTGGCGGTATCGGTATCGCCCCTGGTGCAAACATCGGTGACGAAT > W3110S.gb/1197577‑1197693 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |