| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,207,066 | A→G | 69.2% | E440E (GAA→GAG) | ymfN → | predicted DNA‑binding transcriptional regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,207,066 | 0 | A | G | 69.2% | 33.3 / 18.0 | 26 | E440E (GAA→GAG) | ymfN | predicted DNA‑binding transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base A (5/3); new base G (9/9); total (14/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.83e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.35e-01 | |||||||||||
AAACAAAATCGATGGTGCAGTTGCGCTGATTATGGCGGTTGGCAGAGCCATGCTGTACGAGAAAGAAGACACGCTGTCTGATCACATTGAGTCCTACGGGATCCGCTCGCTTTAACTGAGGTAATTATGATCAT > W3110S.gb/1207000‑1207133 | aaaCAAAATCGATGGTGCAGTTGCGCTGATTATGGCGGTTGGCAGAGCCATGCTGTACGAGAAAGAGGaca < 1:1290818/71‑1 (MQ=255) aaCAAAATCGATGGTGCAGTTGCGCTGATTATGGCGGTTGGCAGAGCCATGCTGTACGAGAAAGAGGacac < 1:2475954/71‑1 (MQ=255) aaCAAAATCGATGGTGCAGTTGCGCTGATTATGGCGGTTGGCAGAGCCATGCTGTACGAGAAAGAAGacac > 1:1882511/1‑71 (MQ=255) aTCGATGGTGCAGTTGCGCTGATTATGGCGGTTGGCAGAGCCATGCTGTACGAGAAAGAGGACACGCTGTc > 1:47409/1‑71 (MQ=255) tGCAGTTGCGCTGATTATGGCGGTTGGCAGAGCCATGCTGTACGAGAAAGAGGACACGCTGTCTGATcaca < 1:564228/71‑1 (MQ=255) tGCAGTTGCGCTGATTATGGCGGTTGGCAGAGCCATGCTGTACGAGAAAGAGGACACGCTGTCTGATcaca < 1:1350058/71‑1 (MQ=255) gTTGCGCTGATTATGGCGGTTGGCAGAGCCATGCTGTACGAGAAAGAGGACACGCTGTCTGATCACAtt > 1:2374991/1‑69 (MQ=255) ttGCTCTGATTATGGCGGTTGGCAGAGCCATGCTGTACGAGAAAGAGGACACGCt < 1:2216013/55‑1 (MQ=255) ttGCGCTGATTATGGCGGTTGTCAGAGCCATGCTGTACGAGAAAGAGGACACGCt < 1:948990/55‑1 (MQ=255) ttGCGCTGATTATGGCGGTTGGCAGAGCCATGCTGTACGAGAAAGAGGACACGCt < 1:1818461/55‑1 (MQ=255) tGCGCTGATTATGGCGGTTGGCAGAGCCATGCTGTACGAGAAAGAGGACACGCTGTCTGATCACATTGAGt > 1:1149858/1‑71 (MQ=255) tGCGCTGATTATGGCGGTTGGCAGAGCCATGCTGTACGAGAAAGAGGACACGCTGTCTGATCACATTGAGt > 1:1913380/1‑71 (MQ=255) gcgcTGATTATGGCGGTTGGCAGAGCCATGCTGTACGAGAAAGAGGACACGCTGTCTGATCACAtt > 1:2557996/1‑66 (MQ=255) cgcTGATTATGGCGGTTGGCAGAGCCATGCTGTACGAGAAAGAGGACACGCTGTCTGATCACATTGAGTcc < 1:1854400/71‑1 (MQ=255) cTGATTATGGCGGTTGGCAGAGCCATGCTGTACGAGAAAGAGGACACGCTGTCTg < 1:2726799/55‑1 (MQ=255) gATTATTGCGGTTGGCAGAGCCATGCTGTACGAGAAAGAAGACACGCTGTCTGATCACATTGAGTCCTACg > 1:6465/1‑71 (MQ=255) agCCATGCTGTACGAGAAAGAAGACACGCTGTCTGATCACATTGAGTCCTAGGGGATCCGCTAGCTTTAAc > 1:170336/1‑71 (MQ=255) tGCTGTACGAGAAAGAAGACACGCTGTCTGATCACATTGAg < 1:1069406/41‑1 (MQ=255) tGCTGTACGAGAAAGAAGACACGCTGTCTGATCACATTGAg < 1:353000/41‑1 (MQ=255) cTGTACGAGAAAGAGGACACGCTGTCTGATCACATTGAGTCCTACgg > 1:238018/1‑47 (MQ=255) cTGTACGAGAAAGAGGACACGCTGTCTGATCACATTGAGTCCTACGGGATCCGCTCGCTTTAACTGAGGt > 1:2013754/1‑70 (MQ=255) cTGTACGAGAAAGAGGACACGCTGTCTGATCACATTGAGTCCTACGGGATCCGCTCGCTTTAACTGAGGt > 1:285181/1‑70 (MQ=255) cTGTACGAGAAAGAAGACACGCTGTCTGATCACATTGAGTCCTACGGGATCCGCTCGCTTTAACTGa > 1:1721399/1‑67 (MQ=255) cTGTACGAGAAAGAAGACACGCTGTCTGATCACATTGAGTCCTACGGGATCCGCTCGCTTTAACTGAGGt > 1:849521/1‑70 (MQ=255) tACGAGAAAGAAGACACGCTGTCTGATCACATTGAGTCCTACGGGATCcgct < 1:897660/52‑1 (MQ=255) cGAGAAAGAGGACACGCTGTCTGATCACATTGAGTCCTACGGGATCCGCTCGCTTTAACTGAGGTAATTAt > 1:700259/1‑71 (MQ=255) gaaagagGACACGCTGTCTGATCACATTGAGTCCTACGGGATCCGCTCGCTTTAACTGAGGTAATTATGAt < 1:1669937/71‑1 (MQ=255) gaaagagGACACGCTGTCTGATCACATTGAGTCCTACGGGATCCGCTCGCTTTAACTGAGGTAATTATGAt < 1:1608230/71‑1 (MQ=255) aaagagGACACGCTGTCTGATCACATTGAGTCCTACGGGATCCGCTCGCTTTAACTGAGGTAATTATGa > 1:860320/1‑69 (MQ=255) aaagaaGACACGCTGTCTGATCACATTGAGTCCTACGGGATCCGCTCGCttt < 1:872452/52‑1 (MQ=255) agaggaCACGCTGTCTGATCACATTGAGTCCTACGGGATCCGCTCGCTTTAACTGAGGTAATTATGATCAt > 1:1713218/1‑71 (MQ=255) agaagaCACGCTGTCTGATCACATTGAGTCCTACGGGATCCGCTCGCTTTaa > 1:1757886/1‑52 (MQ=255) | AAACAAAATCGATGGTGCAGTTGCGCTGATTATGGCGGTTGGCAGAGCCATGCTGTACGAGAAAGAAGACACGCTGTCTGATCACATTGAGTCCTACGGGATCCGCTCGCTTTAACTGAGGTAATTATGATCAT > W3110S.gb/1207000‑1207133 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |