| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,225,448 | G→A | 57.9% | P59P (CCG→CCA) | ycgI → | hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,225,448 | 0 | G | A | 57.9% | 1.7 / 20.6 | 19 | P59P (CCG→CCA) | ycgI | hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base G (3/5); new base A (3/8); total (6/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AGCAGTACGTTTAGCGGACATCTCGGGGTGGGGTATAGCCATGGTGCCGGTGTGGAATCCCCGTGGAACGCGGTGGCTGGTGTGAACTGGTCGTTCTGACCATCAACGATTAAACTGCGCTTCGGCGCAG > W3110S.gb/1225386‑1225515 | agcagTACGTTTAGCGGACATCTCGGGGTGGGGTATAGCCATGGTGCCGGTGTGGAATCCCCGTGGAAcgc < 1:451879/71‑1 (MQ=255)agcagTACGTTTAGCGGACATCTCGGGGTGGGGTATAGCCATGGTGCCGGTGTGGAATCCCCATGGAAcgc < 1:741729/71‑1 (MQ=255)agcagTACGTTTAGCGGACATCTCGGGGTGGGGTATAGCCATGGTGCCGGTGTGGAATCCCCATGGAAcgc < 1:1853449/71‑1 (MQ=255) gTACGTTTAGCGGACATCTCGTGGTGGGGTATAGCCATGGTGCCGGTGTGGAATCCCCATgg < 1:1551194/62‑1 (MQ=255) gTACGTTTAGCGGACATCTCGGGGTGGGGTATAGCCATGGTGCCGGTGTGGAATCCCCATgg < 1:732128/62‑1 (MQ=255) tttAGCGGACATCTCGGGGTGGGGTATAGCCATGGTGCCGGTGTGGAATCCCCGTGGAACGCGGTGGCTgg < 1:2480878/71‑1 (MQ=255) aGCGGACATCTCGGGGTGGGGTATAGCCATGGTGCCGGTGTGGAATCCCCGTGGAAcgc < 1:451878/59‑1 (MQ=255) ggtggggtATATCCATGGTGCCGGTGTGGAATCCCCATGGAACGCGGTGGCTGGTGTGAACTGGTCGTTCt < 1:739780/71‑1 (MQ=255) gtgtATAGCCATGGTGCCGGTGTGGAATCCCCGTGGAACGCGGTGGCTGGTGTGAACTGTTCGTTCTGAc > 1:1571500/3‑70 (MQ=255) taGCCATGGTGCCGGTGTGGAATCCCCGTGGAACGCGGTGGCTGGTGTGAACTGGTCGTTCTg < 1:2356273/63‑1 (MQ=40) gCCATGGTGCCGGTGTGGAATCCCCATGGAACGCGGTGGCTGGTGTGaa > 1:1538253/1‑49 (MQ=255) tGGTGCCGGTGTGGAATCCCCATGGAACGCGGTGGCTGGTTTGAACTGGTCGTTCTGACCATCAACGAt < 1:1951001/69‑1 (MQ=14) tGGTGCCGGTGTGGAATCCCCATGGAACGCGGTGGCTGGTGTGAACTGGTCGTTCTGACCATCAACGAt < 1:1702171/69‑1 (MQ=255) tGGTGCCGGTCTGGAATCCCCGTGGAACGCGGTGGCTGGTGTGAACTGGTCGTTCTGACCATCAACGATTa < 1:2282493/71‑1 (MQ=255) tgGAATCCCCATGGAACGCGGTGGCTGGTGTGAACTGGTCGTTCTGACCATCAACGAtt > 1:988520/1‑59 (MQ=255) tgGAATCCCCATGGAACGCGGTGGCTGGTGTGAACTGGTCGTTCTGACCATCAACGATTa < 1:1889332/60‑1 (MQ=255) gAATCCCCGTGGAACGCGGTGGCTGGTGTGAACTGGTCGTTCTGACCATCAACGATTAAAc > 1:2516219/1‑61 (MQ=255) tCCCCGTGGAACGCGGTGGCTGGTGTGAACTGGTCGTTCTGa > 1:750219/1‑42 (MQ=38) cccATGGAACGCGGTGGCTGGTGTGAACTGGTCGTTCTGACCATCAACGATTAAACTGCGCTTCGGCGCAg > 1:954992/1‑71 (MQ=255) | AGCAGTACGTTTAGCGGACATCTCGGGGTGGGGTATAGCCATGGTGCCGGTGTGGAATCCCCGTGGAACGCGGTGGCTGGTGTGAACTGGTCGTTCTGACCATCAACGATTAAACTGCGCTTCGGCGCAG > W3110S.gb/1225386‑1225515 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |