| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,237,778 | C→T | 86.7% | R347R (CGG→CGA) | ycgB ← | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,237,778 | 0 | C | T | 86.7% | 109.1 / 9.6 | 45 | R347R (CGG→CGA) | ycgB | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base C (3/3); new base T (20/19); total (23/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AGCCAGTCGGAACCGGCGATATCCGGGAACCAGTATTTGTCTTCTTCCGTTGGCGACTGACAAATCCGTTTAATATCCTGGAACATGGCGAACCCGAGGGCATACGGGTTGATGCCGCTGTACCACGGGCTGT > W3110S.gb/1237713‑1237845 | agccagTCGGAACCGGCGATATCCGGGAACCAGTATTTGTCTTCTTCCGTTGGCGACTGACAAATTCGttt < 1:729183/71‑1 (MQ=255)agccagTCGGAACCGGCGATATCCGGGAACCAGTATTTGTCTTCTTCCGTTGGCGACTGACAAATTCGttt < 1:2743248/71‑1 (MQ=255) gccagTCGGAACCGGCGATATCCGGGAACCAGTATTTGTCTTCTTCCGTTGGCGACTGACAAATCCGTTTa < 1:1297359/71‑1 (MQ=255) cagTCGGAACCGGCGATATCCGGGAACCAGTATTTGTCTTCTTCCGTTGGCGACTGACAAATCCGTTTAat < 1:259842/71‑1 (MQ=255) agTCGGAACCGGCGATATCCGGGAACCAGTATTTGTCTTCTTCCGTTGGCGACTGACAAATTCGTTTAata > 1:132984/1‑71 (MQ=255) ccGGCGATATCCGGGAACCAGTATTTGTCTTCTTCCGTTGGCGACTGACAAATTCGTTTAATATCCTGGaa < 1:361967/71‑1 (MQ=255) ggCGATATCCGGGAACCAGTATTTGTCTTCTTCCGTTGGCGACTGACAAATTCGTTTAATATCCTGGAACa > 1:496460/1‑71 (MQ=255) ggCGATATCCGGGAACCAGTATTTGTCTTCTTCCGTTGGCGACTGACAAATTCGTTTAATATCCTGGAACa > 1:2778196/1‑71 (MQ=255) atatCCGGGAACCAGTATTTGTCTTCTTCCGTTGGCGACTGACAAATTCGTTTAATATCCTGGAACa > 1:1650937/1‑67 (MQ=255) tCCGGGAACCAGTATTTGTCTTCTTCCGTTGGCGACTGACAAATTCGTTTAATATCCTGGAACATGGCGa < 1:2500638/70‑1 (MQ=255) gggAACCAGTATTTGTCTTCTTCCGTTGGCGACTGACAAATTCGTTTAATATCCTGGAACATGGCGAAccc < 1:1598446/71‑1 (MQ=255) gAACCAGTATTTGTCTTCTTCCGTTGGCGACTGACAAATTCg < 1:41066/42‑1 (MQ=255) aGTATTTGTCTTCTTCCGTTGGCGACTGACAAATTCGTTTAATATCCTGGaa < 1:2338575/52‑1 (MQ=255) tATTTGTCTTCTTCCGTTGGCGACTGACAAATTCGttt < 1:2463432/38‑1 (MQ=255) tATTTGTCTTCTTCCGTTGGCGACTGACAAATTCGTTTAATATCCTGGAACATGGCGAAccc < 1:2266428/62‑1 (MQ=255) tATTTGTCTTCTTCCGTTGGCGACTGACAAATTCGTTTAATATCCTGGAACATGGCGAACCCGAGGGCa < 1:76166/69‑1 (MQ=255) tATTTGTCTTCTTCCGTTGGCGACTGACAAATTCGTTTAATATCCTGGAACATGGCGAACCCGAGGGCa < 1:1237410/69‑1 (MQ=255) tATTTGTCTTCTTCCGTTGGCGACTGACAAATCCGTTTAATATCCTGGaa < 1:960926/50‑1 (MQ=255) aTTTGTCTTCTTCCGTTGGCGACTGACAAATCCGTTTAATATCCTGGAACATGGCGAACCCGAGGGCATAc > 1:760523/1‑71 (MQ=255) tttGTCTTCTTCCGTTGGCGACTGACAAATTCGtt < 1:2716881/35‑1 (MQ=255) tttGTCTTCTTCCGTTGGCGACTGACAAATTCGTTTAATATCCTGGAACATGGCGAACCCGAGGGCATACg > 1:1952668/1‑71 (MQ=255) tttGTCTTCTTCCGTTGGCGACTGACAAATTCGTTTAATATCCTGGAAAATGGCGAACCCGAGGGCATACg > 1:2039081/1‑71 (MQ=255) tttGTCTTCTTCCGTTGGCGACTGACAAATCCGTTTAATATCCTGGAACATGGCGAACCCGAGGGCATACg > 1:1464/1‑71 (MQ=255) tGTCTTCTTCCGTTGGCGACTGACAAATTCGTTTAat > 1:2138587/1‑37 (MQ=255) tGTCTTCTTCCGTTGGCGACTGACAAATTCGTTTAat > 1:2135281/1‑37 (MQ=255) tGTCTTCTTCCGTTGGCGACTGACAAATTCGTTTAat > 1:2797078/1‑37 (MQ=255) tGTCTTCTTCCGTTGGCGACTGACAAATTCGTTTAATATc > 1:1697999/1‑40 (MQ=255) tGTCTTCTTCCGTTGGCGACTGACAAATTCGTTTAATATCCTGGAACATGGCGAACCCGAGGGCATACggg > 1:1593695/1‑71 (MQ=255) tGTCTTCTTCCGTTGGCGACTGACAAATTCGTTTAATATCCTGGAACATGGCGAACCCGAGGGCATACgg < 1:802403/70‑1 (MQ=255) tGTCTTCTTCCGTTGGCGACTGACAAATCCGTTTAata > 1:19901/1‑38 (MQ=255) tcttctTCCGTTGGCGACTGACAAATTCGTTTAATATCCTGGAACATGGCGAACCCGAGGGCATACGGGtt < 1:2455141/71‑1 (MQ=255) tcttctTCCGTTGGCGACTGACAAATTCGTTTAATATCCTGGAACATGGCGAACCCGAGGGCATACGGGtt < 1:605227/71‑1 (MQ=255) ttcttcCGTTGGCGACTGACAAATTCGTTTAATATCCTGGAACATGGCGaa > 1:442485/1‑51 (MQ=255) ttcttcCGTTGGCGACTGACAAATTCGTTTAATATCCTGGAACATGGCGAACCCGAGGGCATACGGGTTGa > 1:1043325/1‑71 (MQ=255) ttcttcCGTTGGCGACTGACAAATTCGTTTAATATCCTGGAACATGGCGAACCCGAGGGCATACGGGTTGa > 1:2523129/1‑71 (MQ=255) ttcttcAGTTGGCGACTGACAAATTCGTTTAATATCCTGGAACATGGCGAACCCGAGGGCATACGGGTTGa > 1:1877121/1‑71 (MQ=255) ccGTTGGCGACTGACAAATTCGTTTAATATCCTGGAACATGGCGAACCCGAGGGCATACGGGTTGATGCCg > 1:581492/1‑71 (MQ=255) ccGTTGGCGACTGACAAATTCGTTTAATATCCTGGAACATGGCGAACCCGAGGGCATACGGGTTGATGCCg > 1:2075576/1‑71 (MQ=255) ccGTTGGCGACTGACAAATTCGTTTAATATCCTGGAACATGGCGAACCCGAGGGCATACGGGTTGATGCCg > 1:2124461/1‑71 (MQ=255) ccGTTGGCGACTGACAAATTCGTTTAATATCCTGGAACATGGCGAACCCGAGGGCATACGGGTTGATGCCg > 1:2265979/1‑71 (MQ=255) ttGGCGACTGACAAATTCGTTTAATATCCTGGAACATGTCGa < 1:1986909/42‑1 (MQ=38) aaTTCGTTTAATATCCTGGAACATGGCGAACCCGAGGGCATACGGGtt < 1:2662847/48‑1 (MQ=255) aaTTCGTTTAATATCCTGGAACATGGCGAACCCGAGGGCATACGGGTTGATGCCGCTGTACGACGGGCTGt > 1:331051/1‑71 (MQ=255) aaTTCGTTTAATATCCTGGAACATGGCGAACCCGAGGGCATACGGGTTGATGCCGCTGTACCACGGGCTGt < 1:1415949/71‑1 (MQ=255) aaTTCGTTTAATATCATGGAACATGGCGAACCCGAGGGCATACGGGTTGATGCCGCTGTACCACGGGCTGt < 1:2641641/71‑1 (MQ=255) | AGCCAGTCGGAACCGGCGATATCCGGGAACCAGTATTTGTCTTCTTCCGTTGGCGACTGACAAATCCGTTTAATATCCTGGAACATGGCGAACCCGAGGGCATACGGGTTGATGCCGCTGTACCACGGGCTGT > W3110S.gb/1237713‑1237845 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |