| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,265,644 | C→T | 52.9% | L118L (CTC→CTT) | hemA → | glutamyl tRNA reductase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,265,644 | 0 | C | T | 52.9% | ‑4.1 / 21.1 | 17 | L118L (CTC→CTT) | hemA | glutamyl tRNA reductase |
| Reads supporting (aligned to +/- strand): ref base C (5/3); new base T (3/6); total (8/9) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.47e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.01e-01 | |||||||||||
TTTAATGCGTGTTGCCAGCGGCCTGGATTCACTGGTTCTGGGGGAGCCGCAGATCCTCGGTCAGGTTAAAAAAGCGTTTGCCGATTCGCAAAAAGGTCATATGAAGGCCAGCGAACTGGAACGCA > W3110S.gb/1265587‑1265711 | tttAATGCGTGTTGCCAGCGGCCTGGATTCACTGGTTCTGGGGGAGCCGCAGATCCTTGGt < 1:488213/61‑1 (MQ=255)tttAATGCGTGTTGCCAGCGGCCTGGATTCACTGGTTCTGGGGGAGCCGCAGATCCTTGGt < 1:1507340/61‑1 (MQ=255)tttAATGCGTGTTGCCAGCGGCCTGGATTCACTGGTTCTGGGGGAGCCGCAGATCCTTGGt < 1:2436107/61‑1 (MQ=255) tAATGCGTGTTGCCAGCGGCCTGGATTCACTGGTTCTGGGGGAGCCGCAGATCCTCGGTCAGGTTaaaa > 1:1253137/1‑69 (MQ=255) ttGCCAGCGGCCTGGATTCACTGGTTCTGGGGGAGCCGCAGATCCTCGGTCAGGTTAAAAAAGCg < 1:2247422/65‑1 (MQ=255) ttGCCAGCGGCCTGGATTCACTGGTTCTGGGGGAGCCGCAGATCCTCGGTCAGGTTAAAAAAGCg < 1:187716/65‑1 (MQ=255) ttGCCAGCGGCCTGGATTCACTGGTTCTGGGGGAGCCGCAGATCCTCGGTCAGGTTAAAAAAGCGtt > 1:1864211/1‑67 (MQ=255) ggCCTGGATTCACTGGTTCTGGGGGAGCCGCAGATCCTCGGTCAGGTTAAAAAAGCGTTTGCCGATTCGc > 1:1009602/1‑70 (MQ=255) gATTCACTGGTTCTGGGGGAGCCGCAGATCCTTGGTCAGGTTAAAAAAGCGTTTGCCGATTCGCAAAAAgg > 1:2699453/1‑71 (MQ=255) cACTGGTTCTGGGGGAGCCGCAGATCCTTGGTCAGGTTAAAAAAGCGTTTGCCGATTCGCAAAAAGGTCa < 1:1063909/70‑1 (MQ=255) tGGTTCTGGGGGAGCCGCAGATCCTTGGTCAGGTTAAAAAAGCg < 1:1755208/44‑1 (MQ=255) ttCTGGGGGAGCCGCAGATCCTCGGTCAGGTTAAAAAAGCGTTTGCCGATTCGCAAAAAGGTCATATGAAg < 1:1466906/71‑1 (MQ=255) ggAGCCGCAGATCCTTGGTCAGGTTAAAAAAGCGTTTGCCGATTCGCAAAAAGGTCATATGAAGGCCAGCg < 1:863430/71‑1 (MQ=255) tCCTCGGTCAGGTTAAAAAAGCGTTTGCCGATTCGCAAAAAGGTCATATGaa > 1:2054414/1‑52 (MQ=255) ccTTGGTCAGGTTAAAAAAGCGTTTGCCGATTCGCAAAAAg > 1:2543598/1‑41 (MQ=255) ccTTGGTCAGGTTAAAAAAGCGTTTGCCGATTCGCAAAAAGGTCATATGAAGGCCAGCGAACTGGAACGCa > 1:1188727/1‑71 (MQ=255) ccTCGGTCAGGTTAAAAAAGCGTTTGCCGATTCGCAAAAAGGTCATATGAAGGCCAGCGAACTGGAACGCa > 1:1623983/1‑71 (MQ=255) cGGTCAGGTTAAAAAAGCGTTTGCCGATTCGCAAAAAGGTCATATGaa > 1:1575537/1‑48 (MQ=255) | TTTAATGCGTGTTGCCAGCGGCCTGGATTCACTGGTTCTGGGGGAGCCGCAGATCCTCGGTCAGGTTAAAAAAGCGTTTGCCGATTCGCAAAAAGGTCATATGAAGGCCAGCGAACTGGAACGCA > W3110S.gb/1265587‑1265711 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |