Predicted mutation
evidence seq id position mutation freq annotation gene description
RA W3110S.gb 1,274,654 T→C 100% S198P (TCA→CCA)  chaC → regulatory protein for cation transport

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb1,274,6540TC80.0% 16.9 / ‑0.2 10S198P (TCA→CCA) chaCregulatory protein for cation transport
Reads supporting (aligned to +/- strand):  ref base T (0/2);  new base C (1/7);  total (1/9)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.51e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

AGGTCATCGCGCCGTTGATTGCGGCGGCGAGCGGTCCGCTGGGAACCAACGCACAATACCTGTTTTCACTGGAACAGGAGCTTATCAAACTGGGAATGCAGGACGATGGGCTGAATGA  >  W3110S.gb/1274589‑1274706
                                                                 |                                                    
aGGTCATCGCGCCGTTGATTGCGGCGGCGAGCGGTCCGCTGGGAACCAACGCACAATACCTGTTTCCAc                                                   <  1:2316835/69‑1 (MQ=255)
            cGTTGATTGCGGCGGCGAGCGGTCCGCTGGGAACCAACGCACAATACCTGTTTCCACTGGAACAGGAGCtt                                     <  1:1846891/71‑1 (MQ=255)
                    gcggcggcgAGCGGTCCGCTGGGAACCAACGCACAATACCTGTTTCCACTGGAACAGGAGCTTATCAAACt                             >  1:1916539/1‑71 (MQ=255)
                                 gTCCGCTGGGAACCAACGCACAATACCTGTTTCCACTGGAACAGGAGCTTATCaa                                <  1:731713/55‑1 (MQ=255)
                                 gTCCGCTGGGAACCAACGCACAATACCTGTTTCCACTGGAACAGGAGCTTATCAAACTGGGAATGCAGGAc                <  1:1025140/71‑1 (MQ=255)
                                 gTCCGCTGGGAACCAACGCACAATACCTGTTTCCACTGGAACAGGAGCTTATCAAACTGGGAATGCAGGAc                <  1:1883539/71‑1 (MQ=255)
                                  tCCGCTGGGAACCAACGCACAATACCTGTTTTCACTGGAACAGGAGCTTATCAAACTGGGAATGCAGGACg               <  1:2435743/71‑1 (MQ=255)
                                     gCTGGGAACCAACGCACAATACCTGTTTCCACTGGAACAGGAGCTTATCAAACTGGGAATGCAGGACGATg            <  1:152039/71‑1 (MQ=255)
                                               aaCGCACAATACCTGTTTTCACTGGAACAGGAGCTTATCAAACTGGGAATGCAGGACGATGGGCTGAATGa  <  1:266946/71‑1 (MQ=255)
                                                       aTACCTGTTTCCACTGGAACAGGAGCTTATCAAACTGGGAATGc                     <  1:368854/44‑1 (MQ=255)
                                                                ttCACTGGAACAGGAGCTTATCAAACTGGGAATGCAGGACGATGGGCTGaa     >  1:2474142/1‑51 (MQ=255)
                                                                 |                                                    
AGGTCATCGCGCCGTTGATTGCGGCGGCGAGCGGTCCGCTGGGAACCAACGCACAATACCTGTTTTCACTGGAACAGGAGCTTATCAAACTGGGAATGCAGGACGATGGGCTGAATGA  >  W3110S.gb/1274589‑1274706

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑