| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,275,296 | A→G | 73.3% | intergenic (‑120/‑206) | ychN ← / → ychP | conserved hypothetical protein/predicted invasin |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,275,296 | 0 | A | G | 73.3% | 21.4 / 5.9 | 15 | intergenic (‑120/‑206) | ychN/ychP | conserved hypothetical protein/predicted invasin |
| Reads supporting (aligned to +/- strand): ref base A (2/2); new base G (5/6); total (7/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CAGCGTCTGACCAATCGGTCACATTTTTAAGGATTTTCCTGAAAGCGCGAGAAAATACGACAAAAGTTGCCAGTAATCGTTATTCTTTAAGGCTATGGTTTTTCATTATTACCGGAAGTT > W3110S.gb/1275232‑1275351 | cAGCGTCTGACCAATCGGTCACATTTTTAAGGATTTTCCTGAAAGCGCGAGAAAATACGACAAAGGTTGc > 1:2531022/1‑70 (MQ=255) cGTCTGACCAATCGGTCACATTTTTAAGGATTTTCCTGAAAGCGCGAGAAAATACGACAAAGGTTGCCAGt < 1:2617106/71‑1 (MQ=255) ggTCACATTTTTAAGGATTTTCCTGAAAGCGCGAGAAAATACGACAAAAGTTGCCAGTAATCGTTATTCtt > 1:1639078/1‑71 (MQ=255) ggTCACATTTTTAAGGATTTTCCTGAAAGCGCGAGAAAATACGACAAAAGTTGCCAGTAATCGTTATTCtt > 1:1690569/1‑71 (MQ=255) gTCACATTTTTAAGGATTTTCCTGAAAGCGCGAGAAAATACGACAAAGGTTGCCAGAGATCgg < 1:2415375/63‑2 (MQ=255) gTCACATTTTTAAGGATTTTCCTGAAAGCGCGAGAAAATACGACAAAAGTTGCCAGTAATCGt < 1:1086826/63‑1 (MQ=255) aTTTTTAAGGATTTTCCTGAAAGCGCGAGAAAATACGACaaaa < 1:2731640/43‑1 (MQ=255) aTTTTTAAGGATTTTCCTGAAAGCGCGAGAAAATACGACAAAGGTTGCCAGTAATCGTTATTCTTTAAGGc < 1:2113534/71‑1 (MQ=255) tttttAAGGATTTTCCTGAAAGCGCGAGAAAATACGACAAAGGTTGCCAGTAATCGTTATTCTTTAAGGc < 1:270917/70‑1 (MQ=255) aTTTTCCTGAAAGCGCGAGAAAATACGACAAAGGTTGCCAGTAATCGTTa > 1:2075488/1‑50 (MQ=255) ttttCCTGAAAGCGCGAGAAAATACGACAAAGGTTGCCAGTAATCGTTATTCTTTAAGGCTATGGTTTTTc < 1:1377935/71‑1 (MQ=255) ccTGAAAGCGCGAGAAAATACGACAAAGGTTGCCAGTAATCGTTATTCTTTAAGGCTATGGTTTTTCatt < 1:687585/70‑1 (MQ=255) gAAAGCGCGAGAAAATACGACAAAGGTTGCCAGTAATCGTTa > 1:2265701/1‑42 (MQ=255) cgcgAGAAAATACGACAAAAGTTGCCAGTAATCGTTAtt < 1:1081131/39‑1 (MQ=255) cAAAGGTTGCCAGTAATCGTTATTCTTTAAGGCTATGGTTTTTCat > 1:723208/1‑46 (MQ=255) cAAAGGTTGCCAGTAATCGTTATTCTTTAAGGCTATGGTTTTTCat > 1:894625/1‑46 (MQ=255) aaagGTTGCCAGTAATCGTTATTCTTTAAGGCTATGGTTTTTCat > 1:2275377/1‑45 (MQ=255) aaGTTGCCAGTAATCGTTATTCTTTAAGGCTATGGTTTTTCATTATTACCGGAAGtt < 1:1893126/57‑1 (MQ=255) aaGTTGCCAGTAATCGTTATTCTTTAAGGCTATGGTTTTTCATTATTACCGGAAGtt < 1:1043150/57‑1 (MQ=255) | CAGCGTCTGACCAATCGGTCACATTTTTAAGGATTTTCCTGAAAGCGCGAGAAAATACGACAAAAGTTGCCAGTAATCGTTATTCTTTAAGGCTATGGTTTTTCATTATTACCGGAAGTT > W3110S.gb/1275232‑1275351 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |