| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,282,409 | A→G | 81.3% | P323P (CCA→CCG) | narG → | nitrate reductase 1, alpha subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,282,409 | 0 | A | G | 81.3% | 71.8 / 10.6 | 32 | P323P (CCA→CCG) | narG | nitrate reductase 1, alpha subunit |
| Reads supporting (aligned to +/- strand): ref base A (3/3); new base G (12/14); total (15/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.86e-01 | |||||||||||
GCACCGATGCGGCAATGGCGCTGGCGATGGGCCACGTAATGCTGCGTGAATTCCACCTCGACAACCCAAGCCAGTATTTCACCGACTATGTGCGTCGCTACACCGACATGCCGATGCT > W3110S.gb/1282342‑1282459 | gCACCGATGCGGCAATGGCGCTGGCGATGGGCCACGTAATGCTGCGTGAATTCCACCTCGACAACCCGAGc > 1:2530441/1‑71 (MQ=255) ccGATGCGGCAATGGCGCTGGCGATGGGCCACGTAATGCTGCGTGAATTCCACCTCGACAACCCGAGCCAg < 1:257469/71‑1 (MQ=255) gATGCGGCAATGGCGCTGGCGATGGGCCACGTAATGCTGCGTGAATTCCACCTCGACAACCCAAGCCAGTa > 1:1578355/1‑71 (MQ=255) gATGCGGCAATGGCGCTGGCGATGGGCCACGTAATGCTGCGTGAATTCCACCTCGACAACCCAAGCCAGTa > 1:1727316/1‑71 (MQ=255) cGGCAATGGCGCTGGCGATGGGCCACGTAATGCTGCGTGAATTCCACCTCGACAACCCGAGCCAGTAttt > 1:388678/1‑70 (MQ=255) cGGCAATGGCGCTGGCGATGGGCCACGTAATGCTGCGTGAATTCCACCTCGACAACCCGAGCCAGTAttt > 1:2690660/1‑70 (MQ=255) gCAATGGCGCTGGCGATGGGCCACGTAATGCTGCGTGAATTCCACCTCGACAACCCGAGCCAGTATTTCAc > 1:220700/1‑71 (MQ=255) aaTGGCGCTGGCGATGGGCCACGTAATGCTGCGTGAATT‑CACCTCGACAACCCGAGCCAGTAt > 1:963003/1‑63 (MQ=255) aTGGCGCTGGCGATGGGCCACGTAATGCTGCGTGAATTCCACCTCGACAACCCGAGCCAGTATTTCACCGa > 1:2352732/1‑71 (MQ=255) tGGCGCTGGCGATGGGCCACGTAATGCTGCGTGAATTCCACCTCGACAACCCGAGCCAGTATTTCACCGAc > 1:1168155/1‑71 (MQ=255) ggCGCTGGCGATGGGCCACGTAATGCTGCGTGAATTCCACCTCGACAACCCGAGCCAGTATTTCACCGAc > 1:1933777/1‑70 (MQ=255) gcgcTGGCGATGGGCCACGTAATGCTGCGTGAATTCCACCTCGACAACCCGAGCCAGTATTTCACCGACTa < 1:2502220/71‑1 (MQ=255) cgcTGGCGATGGGCCACGTAATGCTGCGTGAATTCCACCTCGACAACCCGAGCCAGTATTTCACCGACTa < 1:2521489/70‑1 (MQ=255) tGGCGATGGGCCACGTAATGCTGCGTGAATTCCACCTCGACAACCCGAGCCAGTATTTCACCGACTAtgtg > 1:1008072/1‑71 (MQ=255) tGGGCCACGTAATGCTGCGTGAATTCCACCTCGACAACCCGAGCCAGTATTTCACCGACTATGTGCGTCGc < 1:1970150/71‑1 (MQ=255) gCCACGTAATGCTGCGTGAATTCCACCTCGACAACCCGAGCCAGTATTTCACCGACTATGTGCGTCGCTac < 1:22926/71‑1 (MQ=255) gCCACGTAATGCTGCGTGAATTCCACCTCGACAACCCGAGCCAGTATTTCACCGACTATGTGCGTCGCTac < 1:1645469/71‑1 (MQ=255) cGTAATGCTGCGTGAATTCCACCTCGACAACCCGAGCCAGTATTTCACCGACt < 1:1943381/53‑1 (MQ=255) gTAATGCTGCGTGAATTCCACCTCGACAACCCGAGCCAGTATTTCACCGACTATGTGCGTCGc < 1:18980/63‑1 (MQ=255) tAATGCTGCGTGAATTCCACCTCGACAACCCGAGCCAGTATTTCACCGAc > 1:2641207/1‑50 (MQ=255) aaTGCTGCGTGAATTCCACCTCGACAACCCGAGCCAGTATTTCAACGACTATGTGCGt > 1:2196635/1‑58 (MQ=255) ctgcGTGAATTCCACCTCGACAACCCGAGCCAGTATTTCACCGACTATGTGCGTCGCTACACCGACATGcc < 1:2749475/71‑1 (MQ=255) ctgcGTGAATTCCACCTCGACAACCCGAGCCAGTATTTCACCGACTATGTGCGTCGCTACACCGACATGcc < 1:2752755/71‑1 (MQ=255) ctgcGTGAATTCCACCTCGACAACCCAAGCCAGTATTTCACCGACTATGTGCGTCGCTACACCGACATGcc > 1:2577528/1‑71 (MQ=255) cGTGAATTCCACCTCGACAACCCGAGCCAGTATTTCACCGACTATGTGCGTCGCTACACCGACATGCCGAt < 1:1653318/71‑1 (MQ=255) aaTTCCACCTCGACAACCCGAGCCAGTATTTCACCGACTATGTGCGTCGCTACACCGACATGcc < 1:1117055/64‑1 (MQ=255) aaTTCCACCTCGACAACCCGAGCCAGTATTTCACCGACTATGTGCGTCGCTACACCGACATGCCGATGCt < 1:1649666/70‑1 (MQ=255) ttCCACCTCGACAACCCAAGCCAGTATTTCACCGACTATGTGCGTCGCTACACCGa < 1:2733049/56‑1 (MQ=255) ttCCACCTCGACAACCCAAGCCAGTATTTCACCGACTATGTGCGTCGCTACACCGACATGCCGATGCt < 1:2241778/68‑1 (MQ=255) ttCCACCTCGACAACCCAAGCCAGTATTTCACCGACTATGTGCGTCGCTACACCGACATGCCGATGCt < 1:522669/68‑1 (MQ=255) aCCTCGACAACCCGAGCCAGTATTTCACCGACTATGTGCGTCGCTACACCGACATGcc < 1:1193460/58‑1 (MQ=255) ccTCGACAACCCGAGCCAGTATTTCACCGACTATGTGCGTCGCTACACCGACATGCCg > 1:305649/1‑58 (MQ=255) | GCACCGATGCGGCAATGGCGCTGGCGATGGGCCACGTAATGCTGCGTGAATTCCACCTCGACAACCCAAGCCAGTATTTCACCGACTATGTGCGTCGCTACACCGACATGCCGATGCT > W3110S.gb/1282342‑1282459 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |