| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 162,916 | C→T | 55.6% | A271V (GCG→GTG) | hrpB → | predicted ATP‑dependent helicase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 162,916 | 0 | C | T | 55.6% | 3.6 / 33.1 | 27 | A271V (GCG→GTG) | hrpB | predicted ATP‑dependent helicase |
| Reads supporting (aligned to +/- strand): ref base C (4/8); new base T (7/8); total (11/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.96e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TTGTCGCTGAACGATCAGCGAAAAGCGATCCTCCCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTGTGCCCAGGAGC > W3110S.gb/162849‑162984 | ttGTCGCTGAACGATCAGCGAAAAGCGATCCTCCCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGTGAc > 1:450161/1‑71 (MQ=255) gTCGCTGAACGATCAGCGAAAAGCGATCCTCCCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGAcc > 1:1019947/1‑70 (MQ=255) tCGCTGAACGATCAGCGAAAAGCGATCCTCCCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGTGACCaa < 1:1478038/71‑1 (MQ=255) cGAAAAGCGATCCTCCCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGTGACCAATATTGCTGAAACCAg < 1:989875/71‑1 (MQ=255) aaaaGCGATCCTCCCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTGCTGAAACCAGtt > 1:423264/1‑71 (MQ=255) gCGATCCTCCCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAAc < 1:265053/71‑1 (MQ=255) gATCCTCCCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGTGACCAATATTGCTGAAACCAGTTTAACCa < 1:1303586/71‑1 (MQ=255) cctccCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGTGACCAata > 1:2626552/1‑47 (MQ=255) cctccCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGTGACCAATATTGCTGAAACCAGTTTAACCAtt > 1:158629/1‑70 (MQ=255) cctccCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAata > 1:2535018/1‑47 (MQ=255) cctccCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCAtt > 1:915397/1‑70 (MQ=255) ctccCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTg < 1:906089/70‑1 (MQ=255) ctccCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTg < 1:862058/70‑1 (MQ=255) aCCGCAAGGGATGCGCAAAGTGGTGCTGGTGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTc < 1:1086278/71‑1 (MQ=255) aCCGCAAGGGATGCGCAAAGTGGTGCTGGTGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTc > 1:793710/1‑71 (MQ=255) aCCGCAAGGGATGCGCAAAGTGGTGCTGGTGACCAATATTGATGAAACCAGTTTAACCATTGAAGGTATTc < 1:1733434/71‑1 (MQ=255) ccGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCg < 1:811824/71‑1 (MQ=255) cAAGGGATGCGCAAAGTGGTGCTGGTGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTa < 1:33709/64‑1 (MQ=255) aGGGATGCGCAAAGTGGTGCTGGTGACCAATATTGCTGAAACCAGTTTAACCAt > 1:874144/1‑54 (MQ=255) aGGGATGCGCAAAGTGGTGCTGGTGACCAATATTGCTGAAACCAGTTTAACCAt > 1:307775/1‑54 (MQ=255) aGGGATGCGCAAAGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCtgg < 1:1604781/71‑1 (MQ=255) tGCGCAAAGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCtggtggtg < 1:1607961/71‑1 (MQ=255) cgcAAAGTGGTGCTGGTGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCtggtggtgg < 1:2123849/70‑1 (MQ=255) cgcAAAGTGGTGCTGGTGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCtgg > 1:1038439/1‑64 (MQ=255) gcAAAGTGGTGCTGGTGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGAt < 1:463501/71‑1 (MQ=255) aGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATtgtg < 1:2718003/71‑1 (MQ=255) gCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTGTGCCCAGGAGc < 1:1806914/70‑1 (MQ=255) | TTGTCGCTGAACGATCAGCGAAAAGCGATCCTCCCGGCACCGCAAGGGATGCGCAAAGTGGTGCTGGCGACCAATATTGCTGAAACCAGTTTAACCATTGAAGGTATTCGTCTGGTGGTGGATTGTGCCCAGGAGC > W3110S.gb/162849‑162984 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |