| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,303,633 | G→T | 100% | Q246Y (CAG→TAT) | oppA → | oligopeptide transporter subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,303,633 | 0 | G | T | 100.0% | 81.2 / NA | 29 | Q246Y (CAG→TAT) | oppA | oligopeptide transporter subunit |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base T (12/17); total (12/17) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
TAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCG > W3110S.gb/1303566‑1303683 | tAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATCAACTATGTa < 1:28543/71‑1 (MQ=255) aacgaacgAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAAc > 1:353785/1‑71 (MQ=255) acgaacgaATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAAcc < 1:2692533/71‑1 (MQ=255) gaacgaaTCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTa < 1:2439644/71‑1 (MQ=255) acgaaTCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTaa > 1:497024/1‑65 (MQ=255) aTCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGcc < 1:283710/71‑1 (MQ=255) aTCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGcc < 1:2497483/71‑1 (MQ=255) aTCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGcc < 1:2517264/71‑1 (MQ=255) ttCTTGAACGCAGCCCGACCTACTGGAACTACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTAtt > 1:2782141/1‑71 (MQ=255) aCGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTg < 1:2721814/71‑1 (MQ=255) cTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATg > 1:436157/1‑71 (MQ=255) tACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTAcc < 1:870128/66‑1 (MQ=255) tACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTAcc < 1:1674934/66‑1 (MQ=255) aCTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGc > 1:2512911/1‑52 (MQ=255) cTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGTCTATTGCTTCTGAAGTTAc > 1:560717/1‑63 (MQ=255) cTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGa > 1:403189/1‑66 (MQ=255) cTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGa > 1:285893/1‑66 (MQ=255) tGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAg > 1:2598487/1‑58 (MQ=255) ggAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAAc > 1:1673895/1‑71 (MQ=255) acGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTAcc < 1:1151226/56‑1 (MQ=255) cgcgAAATCCGTTATTAACTATGTAACCTATTTGCCTa < 1:2004230/38‑1 (MQ=25) gcgAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCg < 1:190439/71‑1 (MQ=255) gAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGAt < 1:2506443/55‑1 (MQ=255) aaaCCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGt < 1:300150/46‑1 (MQ=39) aaaCCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGt < 1:259246/46‑1 (MQ=39) ttattaACTATGTAACCTATTAGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCg < 1:1626777/61‑1 (MQ=255) tattaACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGa < 1:318046/45‑1 (MQ=38) attaACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGa > 1:2012385/1‑44 (MQ=38) attaACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGa > 1:2729078/1‑44 (MQ=38) | TAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCG > W3110S.gb/1303566‑1303683 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |