| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,346,872 | C→T | 41.9% | V529I (GTT→ATT) | gmr ← | modulator of Rnase II stability |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,346,872 | 0 | C | T | 41.9% | 24.0 / 49.4 | 43 | V529I (GTT→ATT) | gmr | modulator of Rnase II stability |
| Reads supporting (aligned to +/- strand): ref base C (12/13); new base T (9/9); total (21/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01 | |||||||||||
CTAAATTGTTGAATAACAGACAGTGCCAGTTCATCATTCTCAATCAGACAACTCTCTGTCAGTTCAACATCTATAGGGCAGTATTCAAAATTGAGTTCCTGGAGAACCTGTTTCAGGGCGGTGAAAATGGTTTGA > W3110S.gb/1346805‑1346939 | cTAAATTGTTGAATAACAGACAGTGCCAGTTCATCATTCTCAATCAGACAACTCTCTGTCAGTTCAACATc < 1:2747878/71‑1 (MQ=255)cTAAATTGTTGAATAACAGACAGTGCCAGTTCATCATTCTCAATCAGACAACTCTCTGTCAGTTCAACATc < 1:473216/71‑1 (MQ=255) aaaTTGTTGAATAACAGACAGTGCCAGTTCATCATTCTCAATCAGACAACTCTCTGTCAGTTCAACATCta > 1:2323666/1‑71 (MQ=255) aaaTTGTTGAATAACAGACAGTGCCAGTTCATCATTCTCAATCAGACAACTCTCTGTCAGTTCAACATCta > 1:1602908/1‑71 (MQ=255) aaTTGTTGAATAACAGACAGTGCCAGTTCATCATTCTCAATCAGACAACTCTCTGTCAGTTCAATATCta < 1:736329/70‑1 (MQ=255) aaTTGTTGAATAACAGACAGTGCCAGTTCATCATTCTCAATCAGACAACTCTCTGTCAGTTCAACATCta < 1:188203/70‑1 (MQ=255) tgAATAACAGACAGTGCCAGTTCATCATTCTCAATCAGACAACCCTCTGTCAGTTCAACATCTATAggg > 1:1254889/1‑69 (MQ=255) tAACAGACAGTGCCAGTTCATCATTCTCAATCAGACAACTCTCTGTCAGTTCAACATCta < 1:1936660/60‑1 (MQ=255) gTGCCAGTTCATCATTCTCAATCAGACAACTCTCTGTCAGTTCAATATCTATAGGGCAGTATTCAAAATTg < 1:1866675/71‑1 (MQ=255) gTGCCAGTTCATCATTCTCAATCAGACAACTCTCTGTCAGTTCAACATCTATAGGGCAGTATTCAAAATTg < 1:2325698/71‑1 (MQ=255) ttCATCATTCTCAATCAGACAACTCTCTGTCAGTTCAACATc < 1:2122510/42‑1 (MQ=255) ttCATCATTCTCAATCAGACAACTCTCTGTCAGTTCAACATCta < 1:1831083/44‑1 (MQ=255) ttCATCATTCTCAATCAGACAAATCTCTGTCAGTTCAACATCTATAGGGCAg < 1:2780451/52‑1 (MQ=255) tcatcaTTCTCAATCAGACAACTCTCTGTCAGTTCAACATCTATAGGGCAGTATTCaa > 1:1823902/1‑58 (MQ=255) catcatTCTCAATCAGACAACTCTCTGTCAGTTCTATATCTATAGGGCAGTATTCAAAATTGAGTTCCTg < 1:2028606/70‑1 (MQ=255) catcatTCTCAATCAGACAACTCTCTGTCAGTTCAATATCta > 1:1862820/1‑42 (MQ=255) catcatTCTCAATCAGACAACTCTCTGTCAGTTCAATATCTATAGGGCAGTATTCAAAATTGAGTTCCTg < 1:2565786/70‑1 (MQ=255) catcatTCTCAATCAGACAACTCTCTGTCAGTTCAACATCTATAGGGCAGTATTCAAAATTGAGTTCCTg < 1:62618/70‑1 (MQ=255) cataatTCTCAATCAGACAACTCTCTGTCAGTTCAACATCTATAGGGCAGTATTCAAAATTGAGTTCCTg > 1:593112/1‑70 (MQ=255) atcatTCTCAATCAGACAACTCTCTGTCAGTTCAATATCta < 1:1266720/41‑1 (MQ=255) tcatTCTCAATCAGACAACTCTCTGTCAGTTCAAc < 1:212382/35‑1 (MQ=255) tcatTCTCAATCAGACAACTCTCTGTCAGTTCAATATCTATAGGGCAGTATTCAAAATTGAGTTCCTg > 1:1194915/1‑68 (MQ=255) tcatTCTCAATCAGACAACTCTCTGTCAGTTCAATATCTATAGGGCAGTATTCAAAATTGAGTTCCTGgag < 1:437015/71‑1 (MQ=255) catTCTCAATCAGACAACTCTCTGTCAGTTCAACATCTATAGGGCAg < 1:1471953/47‑1 (MQ=255) ctcAATCAGACAACTCTCTGTCAGTTCAATATCTATAGGGCAGTAt > 1:1246008/1‑46 (MQ=255) ctcAATCAGACAACTCTCTGTCAGTTCAATATCTATAGGGCAGTAt > 1:348186/1‑46 (MQ=255) ctcAATCAGACAACTCTCTGTCAGTTCAATATCTATAGGGCAGTAt > 1:1429270/1‑46 (MQ=255) ctcAATCAGACAACTCTCTGTCAGTTCAATATCTATAGGGCAGTAt > 1:1620567/1‑46 (MQ=255) tCAGACAACTCTCTGTCAGTTCAATATCTATAGGGCAGTATTCAAAAtt < 1:894875/49‑1 (MQ=255) tCAGACAACTCTCTGTCAGTTCAATATCTATAGGGCAGTATTCAAAATTg < 1:1321067/50‑1 (MQ=255) tCAGACAACTCTCTGTCAGTTCAATATCTATAGGGCAGTATTCAAAATTg < 1:295602/50‑1 (MQ=255) aGACAACTCTCTGTCAGTTCAACATCTATAGGGCAGTATTCAAAAt > 1:803919/1‑46 (MQ=255) tctctGTCAGTTCAACATCTATAGGGCAGTATTCAAAATTGAGTTCCTGGAGAACCTGTTTCAGGGCGGt > 1:2158990/1‑70 (MQ=255) tctGTCAGTTCAATATCTATAGGGCAGTATTCAAAATTGAGTTCCTGGAGAACCTGTTTCAGGGCGGTGa > 1:2010497/1‑70 (MQ=255) tctGTCAGTTCAATATCTATAGGGCAGTATTCAAAATTGAGTTCCTGGAGAACCTGTTTCAGGGCGGTGa > 1:504412/1‑70 (MQ=255) tctGTCAGTTCAACATCTATAGGGCAGTATTCAAAAt > 1:898473/1‑37 (MQ=255) tctGTCAGTTCAACATCTATAGGGCAGTATTCAAAATTGAGTTCCt > 1:1351894/1‑46 (MQ=255) aGTTCAACATCTATAGGGCAGTATTCAAAATTGAGTTCCTGGAGAACCTGTTTCa > 1:1575112/1‑55 (MQ=255) aGTTCAACATCTATAGGGCAGTATTCAAAATTGAGTTCCTGGAGAACCTGTTTCAg > 1:213063/1‑56 (MQ=255) ttCAACATCTATAGGGCAGTATTCAAAATTGAGTTCCTGGAGAACCTGTTTCAGGGCGGTGaa < 1:1355963/63‑1 (MQ=255) cAATATCTATAGGGCAGTATTCAAAATTGAGTTCCTGGAGAACCTGTTTCAGGGCGGTGAAAATGGTTTGa > 1:1598317/1‑71 (MQ=255) cAACATCTATAGGGCAGTATTCAAAATTGAGTTCCTGga > 1:225577/1‑39 (MQ=255) cAACATCTATAGGGCAGTATTCAAAATTGAGTTCCTGGAGAACCTGTTTCAGGGCGGTGAAAATGGTTTg < 1:240303/70‑1 (MQ=255) aaCATCTATAGGGCAGTATTCAAAATTGAGTTCCTGGAGAACCTGTTTCAGGGCGGTGAAAATGGTTTg < 1:506820/69‑1 (MQ=255) | CTAAATTGTTGAATAACAGACAGTGCCAGTTCATCATTCTCAATCAGACAACTCTCTGTCAGTTCAACATCTATAGGGCAGTATTCAAAATTGAGTTCCTGGAGAACCTGTTTCAGGGCGGTGAAAATGGTTTGA > W3110S.gb/1346805‑1346939 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |