| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,427,357 | A→T | 19.0% | D8V (GAT→GTT) | ydaY → | hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,427,357 | 0 | A | T | 19.0% | 43.9 / 6.0 | 21 | D8V (GAT→GTT) | ydaY | hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (11/6); new base T (2/2); total (13/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.18e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CTCAAAATGGATTCTGTTCCGGTCATTGTTCTGTCTGGCCTGACGGATGAGCAGAAGCAGCGATAACGATCAGTATCGCTCCCGTAATGCATTAATCCGTCGCCACATTGAGAAAATGGATGCCAGTTTGCACGT > W3110S.gb/1427289‑1427423 | cTCAAAATGGGTTCTGTTCCGGTCATTGTTCTGTCTGGCCTGACGGATGAGCAGAAGCAGCGATAACGAt > 1:1012634/1‑70 (MQ=255)cTCAAAATGGATTCTGTTCCGGTCATTGTTCTGTCTGGCCTGACGGATGAGCAGAAGCAGCGATAACGATc < 1:2409437/71‑1 (MQ=255) aTGGATTCTGTTCCGGTCATTGTTCTGTCTGGCCTGACGGATGAGCAGAAGCAGCGATAACGATCAg > 1:849127/1‑67 (MQ=255) aTGGATTCTGTTCCGGTCATTGTTCTGTCTGGCCTGACGGATGAGCAGAAGCAGCGATAACGATCAGTATc > 1:2385459/1‑71 (MQ=255) tCTGTTCCGGTCATTGTTCTGTCTGGCCTGACGGATGAGCAGAAGCAGCGATAACGTTCAGTATCGCTccc > 1:2208975/1‑71 (MQ=255) ttCCGGTCATTGTTCTGTCTGGCCTGACGGATGAGCAGAAGCAGCGATAACGTTCAGTATCGCTCCCGt < 1:650096/69‑1 (MQ=255) ttCCGGTCATTGTTCTGTCTGGCCTGACGGATGAGCAGAAGCAGCGATAACGATCAGTATCGCTCCCGt < 1:1130367/69‑1 (MQ=255) ttCCGGTCATTGTTCTGTCTGGCCTGACGGATGAGCAGAAGCAGCGATAACGATCAGTATCGCTCCCGt < 1:2408780/69‑1 (MQ=255) aTTGTTCTGTCTGGCCTGACGGATGAGCAGAAGCAGCGATAACGATCAGTATCGCTCCCGTAATGCATTa < 1:2045595/70‑1 (MQ=255) gTTCTGTCTGGCCTGACGGATGAGCAGAAGCAGCGATAACGATCAGTATCGCTCCCGTAATGCATTAATcc > 1:1028044/1‑71 (MQ=255) ttCTGTCTGGCCTGACGGATGAGCAGAAGCAGCGATAACGTTCAGTATCGCTCCCGTAATGCAtt < 1:1904999/65‑1 (MQ=255) ctgtctgGCCTGACGGATGAGCAGAAGCAGCGATAACGATCAGTATCGCTCCCGTAATGCATTAATCCGTc > 1:1406613/1‑71 (MQ=255) ctgtctgGCCTGACGGATGAGCAGAAGCAGCGATAACGATCAGTATCGCTCCCGTAATGCATTAATCCGTc > 1:1371291/1‑71 (MQ=255) gATGAGCAGAAGCAGCGATAACGTTCAGTATCGCTCCCGTAATGCATTAATCCGTCGCcaca > 1:1073722/1‑62 (MQ=255) tGAGCAGAAGCAGAGATAACGATCAGTATCGCTCCCGTAATGCATTAATCCGTCGCCACATTGAGAAAATg < 1:82827/71‑1 (MQ=255) gAAGCAGCGATAACGATCAGTATCGCTCCCGTAATGCATTAATCCGTCGCCACATTGAGAAAATGGATGc > 1:1044899/1‑70 (MQ=255) agcGATAACGATCAGTATCGCTCCCGTAATGCATTAATCCGTCGCCACATTGAGAAAATGGa > 1:1130345/1‑62 (MQ=255) agcGATAACGATCAGTATCGCTCCCGTAATGCATTAATCCGTCGCCACATTGAGAAAATGGa > 1:488217/1‑62 (MQ=255) gcGATAACGATCAGTATCGCTCCCGTAATGCATTAATCCGTCGCCACATTGAGAAAATGGATGCCAGTTTg < 1:1364163/71‑1 (MQ=255) aaCGATCAGTATCGCTCCCGTAATGCATTAATCCGTCGCCACATTGAGAAAATGGATGCCAGTTTGCACGt > 1:1823498/1‑71 (MQ=255) aCGATCAGTATCGCTCCCGTAATGCATTAATCCGTCGCCACATTGAGAAAATGGATGCCAGTTTGCa > 1:1465413/1‑67 (MQ=255) | CTCAAAATGGATTCTGTTCCGGTCATTGTTCTGTCTGGCCTGACGGATGAGCAGAAGCAGCGATAACGATCAGTATCGCTCCCGTAATGCATTAATCCGTCGCCACATTGAGAAAATGGATGCCAGTTTGCACGT > W3110S.gb/1427289‑1427423 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |