| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,475,513 | A→G | 100% | pseudogene (8408/8622 nt) pseudogene (3110/3324 nt) |
ydbA → ydbA → |
ECK1398:JW5802+JW1402:b4492; predicted outer membrane protein ECK1398:JW1402:b1405; predicted outer membrane protein, C‑ter fragment |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,475,513 | 0 | A | G | 80.0% | 28.8 / 1.6 | 15 | pseudogene (8408/8622 nt) pseudogene (3110/3324 nt) | ydbA ydbA | ECK1398:JW5802+JW1402:b4492; predicted outer membrane protein ECK1398:JW1402:b1405; predicted outer membrane protein, C‑ter fragment |
| Reads supporting (aligned to +/- strand): ref base A (3/0); new base G (7/5); total (10/5) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.05e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
GGACTACGCAGGTAAAGACGGCTGGAGCGCTAGCGCTACGCTGGAAGGCGGGCCGAACCTGAGCTACGCGAAGAGCCAGCGTACGGCAAGCCTGGCAGGCGCAGGCAGTCAGCACTTTAACGTCGATGACGG > W3110S.gb/1475448‑1475579 | ggACTACGCAGGTAAAGACGGCTGGAGCGCTAGCGCTACGCTGGAAGGCGGGCCGAACCTGAGCTAcgcg > 1:944502/1‑70 (MQ=255) gACTACGCAGGTAAAGACGGCTGGAGCGCTAGCGCTACGCTGGAAGGCGGGCCGAACCTGAGCTGcgcg < 1:1560304/69‑1 (MQ=255) aGACGGCTGGAGCGCTAGCGCTACGCTGGAAGGCGGGCCGAACCTGAGCTGcgcg < 1:1484635/55‑1 (MQ=255) gACGGCTGGAGCGCTAGCGCTACGCTGGAAGGCGGGCCGAACCTGAGCTGCGCGaa > 1:309033/1‑56 (MQ=255) gAGCGCTAGCGCTACGCTGGAAGGCGGGCCGAACCTGAGCTGCGCGAAGAGcc < 1:725829/53‑1 (MQ=255) gAGCGCTAGCGCTACGCTGGAAGGCGGGCCGAACCTGAGCTGCGCGAAGAGCCAGCGTACGGCAAGCCTgg < 1:34737/71‑1 (MQ=255) gcgctagcgctaCGCTGGAAGGCGGGCCGAACCTGAGCTGCGCGAAGAGCCAGCGTACGGCAAGCCTgg > 1:2371740/1‑69 (MQ=255) ccGAACCTGAGCTGCGCGAAGAGCCAGCGTACGGCAAGCCTGGCAGGCGCAGGCAGTCAGCACTTTAACGt > 1:1966153/1‑71 (MQ=255) ccGAACCTGAGCTGCGCGAAGAGCCAGCGTACGGCAAGCATGGCAGGCGCAGGCAGTCAGCACTTTAACGt > 1:994656/1‑71 (MQ=255) gAACCTGAGCTGCGCGAAGAGCCAGCGTACGGCAAGCCTGGCAGGCGCAGGCAGTCAGCACTTTAACGTc > 1:1274117/1‑70 (MQ=255) aCCTGAGCTGCGCGAAGAGCCAGCGTACGGCAAGCCTGGCAGGCGCAGGCAGTCAGCACTTTAACGTCGAt < 1:1196104/71‑1 (MQ=255) gAGCTGCGCGAAGAGCCAGCGTACGGCAAGCCTGGCAGGCGCAGGCAGTCAGCACTTTaa > 1:2361591/1‑60 (MQ=255) gAGCTGCGCGAAGAGCCAGCGTACGGCAAGCCTGGCAGGCGCAGGCAGTCAGCACTTTaa > 1:2763163/1‑60 (MQ=255) aGCTACGCGAAGAGCCAGCGTACGGCAAGCCTGGCAGGCGCAGGCAGTCAGCACTTTAACGTCGATGACgg > 1:126322/1‑71 (MQ=255) aGCTACGCGAAGAGCCAGCGTACGGCAAGCCTGGCAGGCGCAGGCAGTCAGCACTTTAACGTCGATGACgg > 1:1308746/1‑71 (MQ=255) | GGACTACGCAGGTAAAGACGGCTGGAGCGCTAGCGCTACGCTGGAAGGCGGGCCGAACCTGAGCTACGCGAAGAGCCAGCGTACGGCAAGCCTGGCAGGCGCAGGCAGTCAGCACTTTAACGTCGATGACGG > W3110S.gb/1475448‑1475579 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |