| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,482,053 | A→G | 100% | D401G (GAT→GGT) | ynbC → | predicted hydrolase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,482,053 | 0 | A | G | 76.9% | 19.7 / 2.7 | 13 | D401G (GAT→GGT) | ynbC | predicted hydrolase |
| Reads supporting (aligned to +/- strand): ref base A (1/2); new base G (8/2); total (9/4) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.03e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.60e-01 | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
GGCTGGCGCGGTATTCGCCAGCGCAAAACCCATTTACAAATACTGATTAAACAAGCCGTTGCCGATCTCCACGCCAAAGGTTTAGCCGTCCGCGTGGTTGACATTGCCGCAGGGCATGGGCGC > W3110S.gb/1481989‑1482111 | ggctggcGCGGTATTCGCCAGCGCAAAACCCATTTACAAATACTGATTAAACAAGCCGTTGCCGGTCTCCa > 1:2109589/1‑71 (MQ=255) cGCCAGCGCAAAACCCATTTACAAATACTGATTAAACAAGCCGTTGCCGGTCTCCACGCCAAAGGTTTAg > 1:104887/1‑70 (MQ=255) cGCCAGCGCAAAACCCATTTACAAATACTGATTAAACAAGCCGTTGCCGGTCTCCACGCCAAAGGTTTAGc > 1:1728216/1‑71 (MQ=255) gcAAAACCCATTTACAAATACTGATTAAACAAGCCGTTGCCGGTCTCCACGCCAAAGGTTTAGCCGTCcgc > 1:2157756/1‑71 (MQ=255) cAAAACCCATTTACAAATACTGATTAAACAAGCCGTTGCCGGTCTCCACGCCAAAgg < 1:1523395/57‑1 (MQ=255) aTTTACAAATACTGATTAAACAAGCCGTTGCCGATCTCCACGCCAAAGGTTTAGCCGTCCGCGTGGTTGAc < 1:309613/71‑1 (MQ=255) tttACAAATACTGATTAAACAAGCCGTTGCCGGTCTCCACGCCAAAGGTTTa < 1:1992266/52‑1 (MQ=255) tACAAATACTGATTAAACAAGCCGTTGCCGGTCTCCACGCCAAAGGTTTAGCCGTCCGCGTGGTTGACAtt > 1:2516310/1‑71 (MQ=255) tACAAATACTGATTAAACAAGCCGTTGCCGATCTCCACGCCAAAGGTTTAGCCGTCCGCGTGGTTGACa > 1:1528276/1‑69 (MQ=255) tACTGATTAAACAAGCCGTTGCCGGTCTCCACGCCAAAGGTTTAGCCGTCCGCGTGGTTGACATTGCCg > 1:1994146/1‑69 (MQ=255) cTGATTAAACAAGCCGTTGCCGGTCTCCACGCCAAAGGTTTAGc > 1:2645535/1‑44 (MQ=255) aTTAAACAAGCCGTTGCCGATCTCCACGCCAAAGGTTTAGCCGTCCGCGTg < 1:912198/51‑1 (MQ=255) aaGCCGTTGCCGGTCTCCACGCCAAAGGTTTAGCCGTCCGCGTGGTTGACATTGCCGCAGGGCATGGgcgc > 1:167581/1‑71 (MQ=255) | GGCTGGCGCGGTATTCGCCAGCGCAAAACCCATTTACAAATACTGATTAAACAAGCCGTTGCCGATCTCCACGCCAAAGGTTTAGCCGTCCGCGTGGTTGACATTGCCGCAGGGCATGGGCGC > W3110S.gb/1481989‑1482111 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |