| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,484,096 | A→C | 100% | F160C (TTC→TGC) | azoR ← | NADH‑azoreductase, FMN‑dependent |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,484,096 | 0 | A | C | 75.2% | 62.9 / 5.5 | 24 | F160C (TTC→TGC) | azoR | NADH‑azoreductase, FMN‑dependent |
| Reads supporting (aligned to +/- strand): ref base A (5/1); new base C (14/4); total (19/5) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
TCCGGACCGTATGCGATCCCTTCGGCGAAGACAAATTTCACATCGGTAATGCCGATAAAGCCGAGGAACGTGGACAGATACGGCGTCACCAGGTCCGTTGGTCCATCTTTGTGGATCCCGCCGCGGCTGG > W3110S.gb/1484030‑1484159 | tCCGGACCGTATGCGATCCCTTCGGCGAAGACAAATTTCACATCGGTAATGCCGATAAAGCCGAGGaa > 1:2236170/1‑68 (MQ=255)tCCGGACCGTATGCGATCCCTTCGGCGAAGACAAATTTCACATCGGTAATGCCGATAAAGCCGAGGCACGt > 1:2072126/1‑71 (MQ=255) gTATGCGATCCCTTCGGCGAAGACAAATTTCACATCGGTAATGCCGATAAAGCCGAGGCACGTGGACAGa < 1:240187/70‑1 (MQ=255) cGATCCCCTCGGCGAAGACAAATTTCACATCGGTAATGCCGATAAAGCCGAGGAACGTGGACAGATAc > 1:1354420/1‑68 (MQ=255) ttCGGCGAAGACAAATTTCACATCGGTAATGCCGATAAAGCCGAGGCACGTGGACAGAt > 1:948726/1‑59 (MQ=255) tCGGCGAAGACAAATTTCACATCGGTAATGCCGATAAAGCCGAGGCACGTGGACAGATACGGCGTCa > 1:985244/1‑67 (MQ=255) gCGAAGACAAATTTCACATCGGTAATGCCGATAAAGCCGAGGCACGTg < 1:761413/48‑1 (MQ=255) gAAGACAAATTTCACATCGGTAATGCCGATAAAGCCGAGGCACGTGGa > 1:1974439/1‑48 (MQ=255) gAAGACAAATTTCACATCGGTAATGCCGATAAAGCCGAGGCACGTGGACAGATACGGCGTCACCAGGTCCg > 1:1295727/1‑71 (MQ=255) aaGACAAATTTCACATCGGTAATTCCGATAAAGCCGAGGAACGTGGACAGATACGGCGTCACCAGGTCCGt > 1:1110686/1‑71 (MQ=255) cAAATTTCACATCGGTAATGCCGATAAAGCCGAGGCACGTGGACAGATACGGCGTCACCAGGTCCGTTgg < 1:175455/70‑1 (MQ=255) aaaTTTCACATCGGTAATGCCGATAAAGCCGAGGCACGTGGACAGATa > 1:2140142/1‑48 (MQ=255) aaaTTTCACATCGGTAATGCCGATAAAGCCGAGGCACGTGGACAGATACGGCGTCACCAGGTCCGTTGGTc > 1:87289/1‑71 (MQ=255) tttCACATCGGTAATGCCGATAAAGCCGAGGCACGTGGACAGa > 1:1815394/1‑43 (MQ=255) tatCACATCGGTAATGCCGATAAAGCCGAGGCACGTGGACAGa > 1:741637/3‑43 (MQ=38) tCACATCGGTAATGCCGATAAAGCCGAGGAACGTGGACAGATACGGCGTCACCAg > 1:1995968/1‑55 (MQ=255) ggTAATGCCGATAAAGCCGAGGCACGTGGACAGATACGGCGTCACCAGGTCCGTTGGTCCATCTTtg > 1:1168104/1‑67 (MQ=255) ggTAATGCCGATAAAGCCGAGGCACGTGGACAGATACGGCGTCACCAGGTCCGTTGGTCCATCTTtg > 1:2550569/1‑67 (MQ=255) gTAATGCCGATAAAGCCGAGGCACGTGGACAGATACGGCGTCACCAGGTCCGTTGGTCCATCTTTGTGGAt < 1:1006362/71‑1 (MQ=255) gCCGATAAAGCCGAGGCACGTGGACAGATACGGCGTCACCAGGTCCGTTGGTCCATCTTTGTGGATCccgc > 1:1189054/1‑71 (MQ=255) gCCGATAAAGCCGAGGCACGTGGACAGATACGGCGTCACCAGGTCCGTTGGTCCATCTTTGTGGATCccgc > 1:1551333/1‑71 (MQ=255) tAAAGCCGAGGAACGTGGACAGATACGGCGTCAcc < 1:772978/35‑1 (MQ=255) ccGAGGAACGTGGACAGATACGGCGTCACCAGGTCTGTTGGTCCATCTTTGTGGATCCCGCCGCGGCTgg > 1:2693177/1‑70 (MQ=255) ccGAGGAACGTGGACAGATACGGCGTCACCAGGTCCGTTGGTCCATCTTTGTGGATCCCGCCGCGGCTgg > 1:1850816/1‑70 (MQ=255) cGAGGCACGTGGACAGATACGGCGTCACCAGGTCCGTTGGTCCATCTTTGTGGATCCCGCCGCGGCTgg > 1:212959/1‑69 (MQ=255) | TCCGGACCGTATGCGATCCCTTCGGCGAAGACAAATTTCACATCGGTAATGCCGATAAAGCCGAGGAACGTGGACAGATACGGCGTCACCAGGTCCGTTGGTCCATCTTTGTGGATCCCGCCGCGGCTGG > W3110S.gb/1484030‑1484159 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |