| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,496,286 | A→G | 69.6% | L167P (CTT→CCT) | ydcI ← | predicted DNA‑binding transcriptional regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,496,286 | 0 | A | G | 69.6% | 30.8 / 13.6 | 23 | L167P (CTT→CCT) | ydcI | predicted DNA‑binding transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base A (3/4); new base G (6/10); total (9/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AAGTAGCGGGTGATTAGGGCGGACAACCAGCTTCAACGATTCAAGAAACAGCAGTTCGTAATTAAGCCCGGTCATCAGTTCAGGATCTGACATCCGACCAATGCCGATATCGATTTCCCCGGTTTT > W3110S.gb/1496222‑1496347 | aaGTAGCGGGTGATTAGGGCGGACAACCAGCTTCAACGATTCAAGAAACAGCAGTTCGTAATTAGGCCCg < 1:2223258/70‑1 (MQ=255) ggCGGACAACCAGCTTCAACGATTCAAGAAACAGCAGTTCGTAATTAGGCCCGGTCATCAGTTCAGGATCt < 1:877675/71‑1 (MQ=255) cGGACAACCAGCTTCAACGATTCAAGAAACAGCAGTTCGTAATTAGGCCCGGTCATCAGTTCAGGATCTGa > 1:1562400/1‑71 (MQ=255) cGGACAACCAGCTTCAACGATTCAAGAAACAGCAGTTCGTAATTAAGCCCGGtcatca > 1:1095234/1‑58 (MQ=255) ccAGCTTCAACGATTCAAGAAACAGCAGTTCGTAATTAAGCCCg < 1:2028252/44‑1 (MQ=255) aGCTTCAACGATTCAAGAAACAGCAGTTCGTAATTAAGCCCGGTCATCAGTTCAGGATCTGACATCCGAcc < 1:670745/71‑1 (MQ=255) tCAACGATTCAAGAAACAGCAGTTCGTAATTAGGCCCGGTCATCAGTTCAGGATCTGACATCCGa > 1:1986755/1‑65 (MQ=255) aaCGATTCAAGAAACAGCAGTTCGTAATTAGGCCCGGTCATCAGTTCAGGATCTGACATCCGACCAATGcc < 1:816840/71‑1 (MQ=255) aaCGATTCAAGAAACAGCAGTTCGTAATTAGGCCCGGTCATCAGTTCAGGATCTGACATCCGACCAATGcc < 1:785560/71‑1 (MQ=255) aCGATTCAAGAAACAGCAGTTCGTAATTAGGCCCGGTCATCAGTTCAgg < 1:1361320/49‑1 (MQ=255) aCGATTCAAGAAACAGCAGTTCGTAATTAGGCCCGGTCATCAGTTCAGGATCTGACATCCGACCAATGCCg < 1:1684990/71‑1 (MQ=255) aCGATTCAAGAAACAGCAGTTCGTAATT‑AGGCCGGTCATCAGTTCAgg < 1:1362002/48‑1 (MQ=38) aaGAAACAGCAGTTCGTAATTAAGCCCGGTCATCAGTTCAGGATCTGACATCCGACCAATGCCGATATc < 1:712624/69‑1 (MQ=255) cagcagTTCGTAATTAGGCCCGGTCATCAGTTCAGGATCTGACa < 1:2450357/44‑1 (MQ=255) agcagTTCGTAATTAAGCCCGGTCATCAGTTCAGGAt > 1:2123006/1‑37 (MQ=255) ttCGTAATTAGGCCCGGTCATCAGTTCAGGATCTGACATCCGACCAATGCCGATATCGAt < 1:71142/60‑1 (MQ=255) ttCGTAATTAAGCCCGGTCATCAGTTCAGGATCTGACATCCGACCAATGCCGATATCGATTTCCCCGGttt > 1:1176407/1‑71 (MQ=255) cGTAATTAGGCCCGGTCATCAGTTCAGGATCTGACATCCGa > 1:2777126/1‑41 (MQ=255) cGTAATTAGGCCCGGTCATCAGTTCAGGATCTGACATCCGACCaa > 1:1043580/1‑45 (MQ=255) cGTAATTAGGCCCGGTCATCAGTTCAGGATCTGACATCCGACCaa > 1:2788995/1‑45 (MQ=255) cGTAATTAGGCCCGGTCATAAGTTCAGGATCTGACATCCGACCAATGCCGATATCGATTTCCCCGGtttt > 1:1488050/1‑70 (MQ=255) ttAGGCCCGGTCATCAGTTCAGGATCTGACATCCGACCaa < 1:2576048/40‑1 (MQ=255) ttAGGCCCGGTCATCAGTTCAGGATCTGACATCCGACCaa < 1:1787613/40‑1 (MQ=255) aaGCCCGGTCATCAGTTCAGGATCTGACATCCGACCaa > 1:2026426/1‑38 (MQ=255) | AAGTAGCGGGTGATTAGGGCGGACAACCAGCTTCAACGATTCAAGAAACAGCAGTTCGTAATTAAGCCCGGTCATCAGTTCAGGATCTGACATCCGACCAATGCCGATATCGATTTCCCCGGTTTT > W3110S.gb/1496222‑1496347 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |