| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 199,072 | T→C | 16.0% | V382A (GTC→GCC) | yaeT → | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 199,072 | 0 | T | C | 16.0% | 108.0 / 16.3 | 51 | V382A (GTC→GCC) | yaeT | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base T (15/27); new base C (6/2); total (21/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.57e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.14e-01 | |||||||||||
CTCGAAAGATGCCGTCCTGCGTCGCGAAATGCGTCAGATGGAAGGTGCATGGCTGGGGAGCGATCTGGTCGATCAGGGTAAGGAGCGTCTGAATCGTCTGGGCTTCTTTGAAACTGTCGATACCGATACCCAACG > W3110S.gb/199004‑199138 | cTCGAAAGATGCCGTCCTGCGTCGCGAAATGCGTCAGATGGAAGGTGCATGGCTGGGGAGCGATCTGGTc < 1:341943/70‑1 (MQ=255) aaaGATGCCGTCCTGCGTCGCGAAATGCGTCAGATGGAAGGTGCATGGCTGGGGAGCGATCTGGTCGATCa < 1:630902/71‑1 (MQ=255) aTGCCGTCCTGCGTCGCGAAATGCGTCAGATGGAAGGTGCATGGCTGGGGAGCGATCTGGTCGATCAGGGt < 1:2746843/71‑1 (MQ=255) ccGTCCTGCGTCGCGAAATGCGTCAGATGGAAGGTGCATGGCTGGGGAGCGATCTGGTCGAt > 1:692942/1‑62 (MQ=255) ccGTCCTGCGTCGCGAAATGCGTCAGATGGAAGGTGCATGGCTGGGGAGCGATCTGGTCGATCAGGGTAAg < 1:1549797/71‑1 (MQ=255) cGTCCTGCGTCGCGAAATGCGTCAGATGGAAGGTGCATGGCTGGGGAGCGATCTGGTCGATCAGGGTAAgg < 1:1236707/71‑1 (MQ=255) tCCTGCGTCGCGAAATGCGTCAGATGGAAGGTGCATGGCTGGGGAGCGATCTGGTCGATCAGGGt < 1:615861/65‑1 (MQ=255) cTGCGTCGCGAAATGCGTCAGATGGAAGGTGCATGGCTGGGGAGCGATCTGGTCGATCAGGGTAAGGAGc > 1:351111/1‑70 (MQ=255) cTGCGTCGCGAAATGCGTCAGATGGAAGGTGCATGGCTGGGGAGCGATCTGGCCGATCAGGGTAAGGAGc < 1:1014915/70‑1 (MQ=255) gCGTCGCGAAATGCGTCAGATGGAAGGTGCATGGCTGGGGAGCGATCTGGTCGATCAGGGTAAGGAGCGTc > 1:142894/1‑71 (MQ=255) cGTCGCGAAATGCGTCAGATGGAAGGTGCATGGCTGGGGAGCGATCTGGTCGATCAGGGTAAGGAGCGTCt > 1:997992/1‑71 (MQ=255) cGTCGCGAAATGCGTCAGATGGAAGGTGCATGGCTGGGGAGCGATCTGGTCGATCAGGGTAAGGAGCGTCt > 1:775837/1‑71 (MQ=255) cGTCGCGAAATGCGTCAGATGGAAGGTGCATGGCTGGGGAGCGATCTGGTCGATCAGGGTAAGGAGCGTCt > 1:635483/1‑71 (MQ=255) tCGCGAAATGCGTCAGATGGAAGGTGCATGGCTGGGGAGCGATCTGGTCg < 1:658288/50‑1 (MQ=255) tCGCGAAATGCGTCAGATGGAAGGTGCATGGCTGGGGAGCGATCTGGTCg < 1:2182903/50‑1 (MQ=255) tCGCGAAATGCGTCAGATGGAAGGTGCATGGCTGGGGAGCGATCTGGTCg < 1:1791551/50‑1 (MQ=255) cgcgAAATGCGTCAGATGGAAGGTGCATGGCTGGGGAGCGATCTGGTCGATAAGGGTAAGGAGCGTCTGa < 1:987110/70‑1 (MQ=255) gcgAAATGCGTCAGATGGAAGGTGCATGGCTGGGGAGCGATCTGGCCGATCAGGGTAAg > 1:1486653/1‑59 (MQ=255) gcgAAATGCGTCAGATGGAAGGTGCATGGCTGGGGAGCGATCTGGCCGATCAGGGTAAg > 1:1389963/1‑59 (MQ=255) gcgAAATGCGTCAGATGGAAGGTGCATGGCTGGGGAGCGATCTGGCCGATCAGGGTAAg > 1:1846735/1‑59 (MQ=255) gcgAAATGCGTCAGATGGAAGGTGCATGGCTGGGGAGCGATCTGGCCGATCAGGGTAAGGAGCGTCTGAAt > 1:1832274/1‑71 (MQ=255) aaaTGCGTCAGATGGAAGGTGCATGGCTGGGGAGCGATCTGGTCGATCAGGGTAAGGAGCGTCTGAATCGt < 1:633692/71‑1 (MQ=255) aaTGCGTCAGATGGAAGGTGCATGGCTGGGGAGCGATCTGGTCGATCAGGGTAAGGAGCGTCTGAATCGt < 1:1185944/70‑1 (MQ=255) aaTGCGTCAGATGGAAGGTGCATGGCTGGGGAGCGATCTGGTCGATCAGGGTAAGGAGCGTCTGAATCGTc > 1:936092/1‑71 (MQ=255) aaTGCGCCAGATGGAAGGTGCATGGCTGGGGAGCGATCTGGTCGATCAGGGTAAGGAGCGTCTGAATCGTc > 1:2235428/1‑71 (MQ=255) gTCAGATGGAAGGTGCATGGCTGGGGAGCGATCTGGCCGATCAGGGTAAGGAGCGTCTGAATCGTCTGGGc < 1:1073176/71‑1 (MQ=255) tCAGATGGAAGGTGCATGGCTGGGGAGCGATCTGGTCGATCAGGGTAAGGAGCGTCTGAATCGTCTGGGc < 1:874746/70‑1 (MQ=255) tCAGATGGAAGGTGCATGGCTGGGGAGCGATCTGGGCGATCAGGGTAAGGAGCGTCTGAATCGTCTGGGct < 1:1536932/71‑1 (MQ=255) aGATGGAAGGTGCATGGCTGGGGAGCGATCTGGTCGATCAGGGTAAGGAGCGTCTGAAt > 1:2729967/1‑59 (MQ=255) gAAGGTGCATGGCTGGGGAGCGATCTGGTCGATCAGGGTAAGGAGCGTCTGAATCGTCTGGGCTTCTTTGa < 1:2782880/71‑1 (MQ=255) aGGTGCATGGCTGGGGAGCGATCTGGTCGATCAGGGTAAGGAGCGTCTGAATCGTCTGGGcttc < 1:1053583/64‑1 (MQ=255) ggTGCATGGCTGGGGAGCGATCTGGTCGATCAGGGTAAGGAGCGTCTGAATCGTc < 1:892829/55‑1 (MQ=255) ggTGCATGGCTGGGGAGCGATCTGGTCGATCAGGGTAAGGAGCGTCTGAATCGTc < 1:520520/55‑1 (MQ=255) ggTGCATGGCTGGGGAGCGATCTGGTCGATCAGGGTAAGGAGCGTCTGAATCGTc < 1:1949260/55‑1 (MQ=255) tGCATGGCTGGGGAGCGATCTGGTCGATCAGGGTAAGGAGCCTCTGAATCGTc > 1:22229/1‑53 (MQ=255) tGGCTGGGGAGCGATCTGGTCGATCAGGGTAAGGAGCGTCTGAATc < 1:807355/46‑1 (MQ=255) tGGCTGGGGAGCGATCTGGTCGATCAGGGTAAGGAGCGTCTGAATCGTCTGGGCTTCTTTGaa < 1:1353376/63‑1 (MQ=255) gggAGCGATCTTGTCGATCAGGGTAAGGAGCGTCTGAATCGTCTGGGCTTCTTTGAAACTGTcgatacc < 1:1691069/69‑1 (MQ=255) gggAGCGATCTGGTCGATCAGGGTAAGGAGCGTCTGAATCGTCTGGGCTTCTTTGAAACTGTcgatacc < 1:1624295/69‑1 (MQ=255) ggAGCGATCTGGTCGATCAGGGTAAGGAGCGTCTGAATCGTCTGGGCTTCTTTGa < 1:183500/55‑1 (MQ=255) ggAGCGATCTGGTCGATCAGGGTAAGGAGCGTCTGAATCGTCTGGGCTTCTTTGAAACTGTcgataccgat < 1:1224226/71‑1 (MQ=255) aGCGATCTGGTCGATCAGGGTAAGGAGCGTCTGAATCGTCTGGGCTTCTTTGAAACTGTcgataccgatac > 1:1507513/1‑71 (MQ=255) aGCGATCTGGTCGATCAGGGTAAGGAGCGTCTGAATCGTCTGGGCTTCTTTGAAACTGTcgataccgatac > 1:1208380/1‑71 (MQ=255) aGCGATCTGGTCGATCAGGGTAAGGAGCGTCTGAATCGTCTGGGCTTCTTTGAAACTGTcgataccgatac > 1:2560803/1‑71 (MQ=255) aGCGATCTGGCCGATCAGGGTAAGGAGCGTCTGAATCGTCTGGGCTTCTTTGAAACTGTcgataccgatac > 1:1308678/1‑71 (MQ=255) cGATCTGGTCGATCAGGGTAAGGAGCGTCTGAATCGTCTGGGCTTCTTTGa < 1:593387/51‑1 (MQ=255) aTCTGGTCGATCAGGGTAAGGAGCGTCTGAATCGTCTGGGCTTCTTTGAAACTGTcgatacc < 1:1957699/62‑1 (MQ=255) cTGGTCGATCAGGGTAAGGAGCGTCTGAATCGTCTGGGCTTCTTTGAAACTGTCGATACCGATACCCAACg > 1:2516839/1‑71 (MQ=255) cTGGTCGATCAGGGTAAGGAGCGTCTGAATCGTCTGGGCTTCTTTGAAACTGTCGATACCGATACCCAACg > 1:1257749/1‑71 (MQ=255) cTGGCCGATCAGGGTAAGGAGCGTCTGAATCGTCTGGGCTTCTTTGAAACTGTCGATACCGATACCCAACg > 1:1149489/1‑71 (MQ=255) gTCGATCAGGGTAAGGAGCGTCTGAATCGTCTGGGCTTCTTTGAAACTGTc < 1:1991721/51‑1 (MQ=255) | CTCGAAAGATGCCGTCCTGCGTCGCGAAATGCGTCAGATGGAAGGTGCATGGCTGGGGAGCGATCTGGTCGATCAGGGTAAGGAGCGTCTGAATCGTCTGGGCTTCTTTGAAACTGTCGATACCGATACCCAACG > W3110S.gb/199004‑199138 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |