| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,507,250 | A→G | 25.6% | I182T (ATC→ACC) | ydcO ← | predicted benzoate transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,507,250 | 0 | A | G | 25.6% | 62.3 / 24.4 | 39 | I182T (ATC→ACC) | ydcO | predicted benzoate transporter |
| Reads supporting (aligned to +/- strand): ref base A (16/13); new base G (4/6); total (20/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.80e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.32e-01 | |||||||||||
TCAGGGGTAATATAAGTGGGGAGAACGGGTTTAAAGACAACATCAGTTGTGACAACGTCACCTTGCGCGATGACGATCACGATCCCAATAATCATCGCGGCAATTACCGCATAGCGCGGCGCAACGGCCTTGGTTGC > W3110S.gb/1507181‑1507317 | tCAGGGGTAATATAAGTGGGGAGAACGGGTTTAAAGACAACATCAGTTGTGACAACGTCACCTTGCGCGAt < 1:507520/71‑1 (MQ=255) ggTAATATAAGTGGGGAGAACGGGTTTAAAGACAACATCAGTTGTGACAACGTCACCTTGCGCGGTGacg < 1:2641677/70‑1 (MQ=255) tAATATAAGTGGGGAGAACGGGTTTAAAGACAACATCAGTTGTGACAACGTCACCTTGCGCGATGacg < 1:434652/68‑1 (MQ=255) atatAAGTGGGGAGAACGGGTTTAAAGACAACATCAGTTGTGACAACGTCACCTTGCGCGGTGacgatcac > 1:716022/1‑71 (MQ=255) atatAAGTGGGGAGAACGGGTTTAAAGACAACATCAGTTGTGACAACGTCACCTTGCGCGGTGacgatcac > 1:169674/1‑71 (MQ=255) atatAAGTGGGGAGAACGGGTTTAAAGACAACATCAGTTGTGACAACGTCACCTTGCGCGATGacgatcac > 1:2753207/1‑71 (MQ=255) atatAAGTGGGGAGAACGGGTTTAAAGACAACATCAGTTGTGACAACGTCACCTTGCGCGATGacgat > 1:2138628/1‑68 (MQ=255) ataAGTGGGGAGAACGGGTTTAAAGACAACATCAGTTGTGACAACGTCACCTTGCGCGa > 1:1319462/1‑59 (MQ=255) taAGTGGGGAGAATGGGTTTAAAGACAACATCAGTTGTGACAACGTCACCTTGCGCGATGacgatcacgat > 1:2051364/1‑71 (MQ=255) taAGTGGGGAGAACGGGTTTAAAGACAACATCAGTTGTGACAACGTCACCTTGCGCGGTGac > 1:1799487/1‑62 (MQ=255) taAGTGGGGAGAACGGGTTTAAAGACAACATCAGTTGTGACAACGTCACCTTGCGCGATGacgatcacgat > 1:1083891/1‑71 (MQ=255) gaACGGGTTTAAAGACAACATCAGTTGTGACAACGTCACCTTGCGCGATGACGATCACGATCCCAATAatc < 1:2102549/71‑1 (MQ=255) gaACGGGTTTAAAGACAACATCAGTTGTGACAACGTCACCTTGCGCGATGACGATCACGATCCCAATAatc < 1:1429170/71‑1 (MQ=255) aCGGGTTTAAAGACAACATCAGTTGTGACAACGTCACCTTGCGCGATGACGATCACGATCCCAATAatca > 1:1068853/1‑70 (MQ=255) aaaGACAACATCAGTTGTGACAACGTCACCTTGCGCGGTGACGATCACGATCCCAATAATCATCGCGGCa < 1:862139/70‑1 (MQ=255) aaaGACAACATCAGTTGTGACAACGTCACCTTGCGCGATGACGATCACGATCCCAATAATCATCGCGGCa < 1:456749/70‑1 (MQ=255) aaGACAACATCAGTTGTGACAACGTCACCTTGCGCGATGACGATCACGATCCCAATAATCATCGCGGCAAt < 1:1910/71‑1 (MQ=255) acaacaTCAGTTGTGACAACGTCACCTTGCGCGATGACGATCACGATCCCAATAATCATCGCGGCAATTAc > 1:203180/1‑71 (MQ=255) aacaTCAGTTGTGACAACGTCACCTTGCGCGGTGACGATCACGATCCCAATAATCATCGCGGCAATTACCg < 1:1137900/71‑1 (MQ=255) aacaTCAGTTGTGACAACGTCACCTTGCGCGGTGACGATCACGATCCCAATAATCATCGCGGCAATTACCg < 1:995827/71‑1 (MQ=255) aacaTCAGTTGTGACAACGTCACCTTGCGCGATGACGATCACGATCCCAATAATCATCGCGGCAATTACCg < 1:1914504/71‑1 (MQ=255) aTCAGTTGTGACAACGTCACCTTGCGCGATGacgatcacga > 1:1436501/1‑41 (MQ=255) aTCAGTTGTGACAACGTCACCTTGCGCGATGACGATCACGATCCCAATAATCATCGCGGCAATTACCGCa > 1:1018110/1‑70 (MQ=255) aTCAGTTGTGACAACGTCACCTTGCGCGATGACGATCACGATCCCAATAATCATCGCGGCAATTACCGCa > 1:2785465/1‑70 (MQ=255) aGTTGTGACAACGTCACCTTGCGCGGTGACGATCACGATCCCAATAATCATCGCGGCAATTACCGCATAgc > 1:1049798/1‑71 (MQ=255) ttGTGACAACGTCACCTTGCGCGATGACGATCACGATCCCAATAATCATCGCGGCAATTACCGc < 1:1185977/64‑1 (MQ=255) tgtgACAACGTCACCTTGCGCGATGACGATCACGATCCCAATAATCATCGCGGCAAt < 1:1765829/57‑1 (MQ=255) aCAACGTCACCTTGCGCGATGACGATCACGATCCCAATACTCATCGCGGCAATTa > 1:2628973/1‑55 (MQ=255) aCAACGTCACCTTGCGCGATGACGATCACGATCCCAATAATCATCGCGGCAATTa > 1:690235/1‑55 (MQ=255) cAACGTCACCTTGCGCGGTGACGATCACGATCCCAATAATCATCGCGGCAATTACCGCATAGCGCGGCGCa < 1:1376169/71‑1 (MQ=255) cAACGTCACCTTGCGCGATGACGATCACGATCCCAATAATCATCGCGGCAATTACCGCATAGCGCGGCGCa < 1:1380921/71‑1 (MQ=255) aaCGTCACCTTGCGCGATGACGATCACGATCCCAATAATCATCGCGGCAATTa > 1:1785136/1‑53 (MQ=255) aaCGTCACCTTGCGCGATGACGATCACGATCCCAATAATCATCGCGGCAATTa > 1:847455/1‑53 (MQ=255) aaCGTCACCTTGCGCGATGACGATCACGATCCCAATAATCATCGCGGCAATTAc > 1:1526/1‑54 (MQ=255) aaCGTCACCTTGCGCGATGACGATAACGATCCCAATAATCATCGCGGCAATTAc > 1:1392715/1‑54 (MQ=255) gTCACCTTGCGCGATGACGATCACGATCCCAATAATCATCGCGGCAATTACCGCATAGCGCGGCGCAACgg < 1:1266169/71‑1 (MQ=255) aCCTTGCGCGATGACGATCACGATCCCAATAATCATCGCGGCAATTACCGCATAGCGCGGCGCAACGGCCt < 1:879669/71‑1 (MQ=255) tGCGCGATGACGATCACGATCCCAATAATCATCGCGGCAATTACCGCATAGCGCGGCGCAACGGCCTTGGt > 1:2479661/1‑71 (MQ=255) cgcgATGACGATCACGATCCCAATAATCATCGCGGCAATTACCGCATAGCGCGGCGCAACGGCCTTGGTTg < 1:2512304/71‑1 (MQ=255) gcgGTGACGATCACGATCCCAATAATCATCGCGGCAATTACCGCATAGCGCGGCGCAACGGCCTTGGTTgc < 1:418573/71‑1 (MQ=255) aTGACGATCACGATCCCAATAATAATCGCGGCAATTACCGCATAGCGCGGCGCAACGGCCTTGGTTgc > 1:15125/1‑68 (MQ=255) | TCAGGGGTAATATAAGTGGGGAGAACGGGTTTAAAGACAACATCAGTTGTGACAACGTCACCTTGCGCGATGACGATCACGATCCCAATAATCATCGCGGCAATTACCGCATAGCGCGGCGCAACGGCCTTGGTTGC > W3110S.gb/1507181‑1507317 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |