| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,538,270 | A→G | 69.2% | F21S (TTT→TCT) | narV ← | nitrate reductase 2 (NRZ), gamma subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,538,270 | 0 | A | G | 69.2% | 33.4 / 18.1 | 26 | F21S (TTT→TCT) | narV | nitrate reductase 2 (NRZ), gamma subunit |
| Reads supporting (aligned to +/- strand): ref base A (4/4); new base G (10/8); total (14/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ATTTGACTTGAGGAGGCGCGCCAGGTGTACTGCCCGTAGTCGTAGCGCAGCCAGCTGCCGAGGAAAAACACCGTCGCACAAATGTACGGGTAGATATCGTAAAAAAAGACGTTCAGATACTGAATCATTTCCC > W3110S.gb/1538204‑1538336 | aTTTGACTTGAGGAGGCGCGCCAGGTGTACTGCCCGTAGTCGTAGCGCAGCCAGCTGCCGAGGAAAAacac < 1:2575226/71‑1 (MQ=255) gaggagGCGCGCCAGGTGTACTGCCCGTAGTCGTAGCGCAGCCAGCTGCCGAGGAAAGACACCGTCGcac > 1:1471176/1‑70 (MQ=255) aggCGCGCCAGGTGTACTGCCCTTAGTCGTAGCGCAGCCAGCTGCCGAGGAAAGACACCGTCGCACAAATg < 1:1782119/71‑1 (MQ=255) aggCGCGCCAGGTGTACTGCCCGTAGTCGTAGCGCAGCCAGCTGCCGAGGAAAGACACCGTCGCACAAATg < 1:356417/71‑1 (MQ=255) ccAGGTGTACTGCCCGTAGTCGTAGCGCAGCCAGCTGCCGAGGAAAGac < 1:1583198/49‑1 (MQ=255) ggTGTACTGCCCGTAGTCGTAGCGCAGCCAGCTGCCGAGGAAAGACACCGTCGCACAAATGTACggg > 1:2013732/1‑67 (MQ=255) gtACTGCCCGTAGTCGTAGCGCAGCCAGCTGCCGAGGAAAAACACCGTCGCACAAATGTACGGGTAGata > 1:1543584/1‑70 (MQ=255) cTGCCCGTAGTCGTAGCGCAGCCAGCTGCCGAGGAAAGACACCGTCGCACAAATGTACGGGTAGATATCg > 1:1520383/1‑70 (MQ=255) cTGCCCGTAGTCGTAGCGCAGCCAGCTGCCGAGGAAAAACACCGTCGCACAAATGTACGGGTAGATATCg > 1:1108229/1‑70 (MQ=255) cccGTAGTCGTAGCGCAGCCAGCTGCCGAGGAAAGACACCGTCGCACAAATGTACGGGTAGATATCGTaa > 1:930342/1‑70 (MQ=255) cccGTAGTCGTAGCGCAGCCAGCTGCCGAGGAAAGACACCGTCGCACAAATGTACGGGTAGATATCGTaa > 1:1036686/1‑70 (MQ=255) tAGTCGTAGCGCAGCCAGCTGCCGAGGAAAGacac < 1:1725103/35‑1 (MQ=255) tAGTCGTAGCGCAGCCAGCTGACGAGGAAAAACACCGTCGGACaa < 1:1403901/45‑1 (MQ=38) gTAGCGCAGCCAGCTGCCGAGGAAAAACACCGTCGCACAAATGTACGGGTAGATATCGTAAAAAAAGACGt > 1:1158327/1‑71 (MQ=255) gTAGCGCAGCCAGCTGCCGAGGAAAAACACCGTCGCACAAATGTACGGGTAGATATCGTAAAAAAAGACGt > 1:1882470/1‑71 (MQ=255) gcgcAGCCAGCTGCCGAGGAAAAACACCGTCGCACAAATGTACGGGTAGATATCGTAAAAAAAGACGTTCa < 1:3911/71‑1 (MQ=255) gcAGCCAGCTGCCGAGGAAAGACACCGTCGCACAAATGTACGGGTAGATATCGTAAAAAAAGACGtt > 1:1309193/1‑67 (MQ=255) ccagcTGCCGAGGAAAGACACCGTCGCACAAATGTACGGGTAGATATCGTAAAAAAAGa > 1:2472246/1‑59 (MQ=255) cTGCCGAGGAAAGACACCGTCGCACAAATGTACGGGTAGATATCGTAAAAAAAGa < 1:242209/55‑1 (MQ=255) cTGCCGAGGAAAGACACCGTCGCACAAATGTACGGGTAGATATCGTAAAAAAAGa < 1:2030361/55‑1 (MQ=255) cTGCCGAGGAAAAACACCGTCGCACAAATGTACGGGTAGATATCGTAAAAAAAGACGTTCAGATACTGAAt < 1:213139/71‑1 (MQ=255) gCCGAGGAAAGACACCGTCGCACAAATGTACGGGTAGATATCGTAAAAAAAGCCGTTCAGATACTGAATCa > 1:612595/1‑71 (MQ=255) gCCGAGGAAAGACACCGTCGCACAAATGTACGGGTAGATATCGTAAAAAAAGACGTTCAGATACTGAATCa > 1:1740463/1‑71 (MQ=255) gCCGAGGAAAGACACCGTCGCACAAATGTACGGGTAGATATCGTAAAAAAAGACGTTCAGATACTGAATCa > 1:1837782/1‑71 (MQ=255) ggAAAGACACCGTCGCACAAATGTACGGGTAGATATCGTAAAAAAAGACGTTCAGATACTGAATCATTTcc < 1:2616492/71‑1 (MQ=255) gAAAGACACCGTCGCACAAATGTACGGGTAGATATCGTAAAAAAAGACGTTCAGATACTGAATCATTTccc < 1:2503207/71‑1 (MQ=255) aaaaaCACCGTCGCACAAATGTACGGGTAGATATCGTAAAAAAAGACGTTCAGATACTGAATCa < 1:742354/64‑1 (MQ=255) aaaCACCGTCGCACAAATGTACGGGTAGATATCGTAAAAAAAGACGTTCAGa > 1:1892331/1‑52 (MQ=255) | ATTTGACTTGAGGAGGCGCGCCAGGTGTACTGCCCGTAGTCGTAGCGCAGCCAGCTGCCGAGGAAAAACACCGTCGCACAAATGTACGGGTAGATATCGTAAAAAAAGACGTTCAGATACTGAATCATTTCCC > W3110S.gb/1538204‑1538336 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |