| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,575,785 | C→T | 40.0% | A378T (GCT→ACT) | pqqL ← | predicted peptidase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,575,785 | 0 | C | T | 40.0% | 25.6 / 43.4 | 40 | A378T (GCT→ACT) | pqqL | predicted peptidase |
| Reads supporting (aligned to +/- strand): ref base C (12/12); new base T (9/7); total (21/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.55e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.75e-01 | |||||||||||
AGCTTGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGGGTAGATTTGACATCATCGAGTTCTTCAGCAGAAAAACCATGCTGATCAATGGTTGCCAACTCTGCCATTAATGCATTCGCAGCATCCTGCATATT > W3110S.gb/1575717‑1575851 | aGCTTGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGGGTAGATTTGACATCATCGAGTTCTTcagcag < 1:2319030/71‑1 (MQ=255) cTTGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGGGTAGATTTGACATCATCGAGTTCTTCAGCAGaa < 1:2517466/71‑1 (MQ=255) aTCAACCGCATTTTTCAGCCAGGTGAGGCGGGTAGATTTGACATCATCGAGTTCTTCAGTAGAAAAAc < 1:1629811/68‑1 (MQ=255) aTCAACCGCATTTTTCAGCCAGGTGAGGCGGGTAGATTTGACATCATCGAGTTCTTCAGCAGAAAAACCAt > 1:1820086/1‑71 (MQ=255) gCATTTTTCAGCCAGGTGAGGCGGGTAGATTTGACATCATCGAGTTCTTCAGCAGAAAAACCATGCTGAt > 1:861372/1‑70 (MQ=255) gCATTTTTCAGCCAGGTGAGGCGGGTAGATTTGACATCATCGAGTTCTTCAGCAGAAAAACCATGCTGAt > 1:178457/1‑70 (MQ=255) tttttCAGCCAGGTGAGGCGGGTAGATTTGACATCATCGAGTTCTTCAGTAGAAAAACCATGCTGATCAAt > 1:2176402/1‑71 (MQ=255) tttttCAGCCAGGTGAGGCGGGTAGATTTGACATCATCGAGTTCTTCAGTAGAAAAACCATGCTGATCAAt > 1:1285941/1‑71 (MQ=255) tttCAGCCAGGTGAGGCGGGTAGATTTGACATCATCGAGTTCTTCAGCAGAAAAACCATGCTGATCaa > 1:2139008/1‑68 (MQ=255) ttCAGCCAGGTGAGGCGGGTAGATTTGACATCATCGAGTTCTTCAGTAGAAAAACCATGCTGATCAATGGt > 1:2312620/1‑71 (MQ=255) ttCAGCCAGGTGAGGCGGGTAGATTTGACATCATCGAGTTCTTCAGCAGAAAAACCATGCTGATCAATgg < 1:1258870/70‑1 (MQ=255) gCCAGGTGAGGCGGGTAGATTTGACATCATCGAGTTCTTCAGCAGAAAAACCATGCTGATCAATGGTTGcc < 1:1059480/71‑1 (MQ=255) cAGGTGAGGCGGGTAGATTTGACATCATCGAGTTCTTCAGCAGAAAAACCATGCTGATCAATGGTTGCCaa < 1:1936874/71‑1 (MQ=255) gTGAGTCGGGTAGATTTGACATCATCGAGTTCTTCAGCAGAAAAACCATGCt < 1:743343/52‑1 (MQ=255) gTGAGGCGGGTAGATTTGACATCATCGAGTTCTTCAGCAGAAAAACCATGCt < 1:887174/52‑1 (MQ=255) gTGAGGCGGGTAGATTTGACATCATCGAGTTCTTCAGCAGAAAAACCATGCt < 1:2387597/52‑1 (MQ=255) gAGGCGGGTAGATTTGACATCATCGAGTTCTTCAGTAGaaaaa > 1:1280667/1‑43 (MQ=255) gAGGCGGGTAGATTTGACATCATCGAGTTCTTCAGCAGAAAAACCATGCTGa > 1:623992/1‑52 (MQ=255) gggTAGATTTGACATCATCGAGTTCTTCAGCAGAAAAACCATGCTGATc < 1:1668543/49‑1 (MQ=255) gggTAGATTTGACATCATCGAGTTCTTCAGCAGAAAAACCATGCTGATc < 1:2396206/49‑1 (MQ=255) gTAGATTTGACATCATCGAGTTCTTCAGCAGAAAAACCATGCTGATCAATGGTTGCCAACTCTGCCAtt < 1:1830608/69‑1 (MQ=255) tAGATTTGACATCATCGAGTTCTTCAGCAGAAAAACCATGCTGATCAATGGTTGCCAACTCTGCCATTAAt > 1:1334603/1‑71 (MQ=255) gATTTGACATCATCGAGTTCTTCAGTAGAAAAACCATGCTGATc < 1:1123240/44‑1 (MQ=255) gATTTGACATCATCGAGTTCTTCAGCAGAAAAACCATGCTGATCAATGGTTGCCAACTCTGCCATTAATg > 1:1554322/1‑70 (MQ=255) tttGACATCATCGAGTTCTTCAGCAGAAAAACCATGCTGATCaa > 1:1114384/1‑44 (MQ=255) tttGACATCATCGAGTTCTTCAGCAGAAAAACCATGCTGATCAATGGTTGCCAActc > 1:412490/1‑57 (MQ=255) ttGACATCATCGAGTTCTTCAGTAGAAAAACCATGCTGATCAATGGTTGCCAACTCTGCCATTAATGCAtt > 1:2726931/1‑71 (MQ=255) aCATCATCGAGTTCTTCAGTAGAAAAACCATGCTGATCAATGGTTGCCAACTCTGCCATTAATGCATTCgc < 1:478283/71‑1 (MQ=255) catcatCGAGTTCTTCAGTAGAAAAACCATGCTGATCAATGGTTGCCAACTCTGCCATTAATGCATTCgc < 1:1863865/70‑1 (MQ=255) catcatCGAGTTCTTCAGCAGAAAAACCATGCTGATCAATGGTTGCCAACTCTGc < 1:2182321/55‑1 (MQ=255) tcatcGAGTTCTTCAGTAGAAAAACCATGCTGATCAATGGTTGCCAACTCTGCCATTAATGCATTCgcag < 1:1847625/70‑1 (MQ=255) tcatcGAGTTCTTCAGCAGAAAAACCATGCTGATCAATgg > 1:1623756/1‑40 (MQ=255) atcGAGTTCTTCAGTAGAAAAACCATGCTGATCaa > 1:2029879/1‑35 (MQ=255) atcGAGTTCTTCAGTAGAAAAACCATGCTGATCaa > 1:2780151/1‑35 (MQ=255) atcGAGTTCTTCAGTAGAAAAACCATGCTGATCAATGGTTGCCAACTCTGCCATTAATGCATTCGCAGCAt > 1:339608/1‑71 (MQ=255) atcGAGTTCTTCAGCAGAAAAACCATGCTGATCaa > 1:678516/1‑35 (MQ=255) atcGAGTTCTTCAGCAGAAAAACCATGCTGATCAATGGTTGCCAACTCTGCCATTAAt > 1:939531/1‑58 (MQ=255) gAGTTCTTCAGTAGAAAAACCATGCTGATCAATGGTTGCCAACTCTGCCATTAATGCATTCGCAGCATCCt > 1:222286/1‑71 (MQ=255) ttcttcAGTAGAAAAACCATGCTGATCAATGGTTGCCAACTCTGCCATTAATg < 1:110095/53‑1 (MQ=255) ttcttcAGCAGAAAAACCATGCTGATCAATGGTTGCCAACTCTGCCATTAATGCATTCGCAGCATCCTGCa < 1:1690050/71‑1 (MQ=255) cttcAGTAGAAAAACCATGCTGATCAATGGTTGCCaa < 1:1476675/37‑1 (MQ=255) cagtagAAAAACCATGCTGAGCAATGGTTGCCAACTCTGCCATTAATGCATTCGCAGCATCCTGCAtatt < 1:122311/70‑1 (MQ=255) cagctgAAAAACCATGCTGATCAATGGTTGCCAACTCTGCCATTAATGCATTCGCAGCATCCTGCAtatt < 1:2733522/70‑1 (MQ=255) | AGCTTGCTGATCAACCGCATTTTTCAGCCAGGTGAGGCGGGTAGATTTGACATCATCGAGTTCTTCAGCAGAAAAACCATGCTGATCAATGGTTGCCAACTCTGCCATTAATGCATTCGCAGCATCCTGCATATT > W3110S.gb/1575717‑1575851 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |