| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,608,013 | G→A | 42.9% | G67E (GGA→GAA) | lsrF → | predicted aldolase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,608,013 | 0 | G | A | 42.9% | 6.8 / 22.4 | 21 | G67E (GGA→GAA) | lsrF | predicted aldolase |
| Reads supporting (aligned to +/- strand): ref base G (4/8); new base A (3/6); total (7/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.31e-01 | |||||||||||
TCCGAAAACGGGTAAAACCGTGATGCTGGCTTTTGACCATGGTTATTTTCAGGGACCGACTACCGGACTTGAACGCATTGATATAAATATCGCCCCGCTGTTTGAACATGCCGATGTATTAATGT > W3110S.gb/1607948‑1608072 | tCCGAAAACGGGTAAAACCGTGATGCTGGCTTTTGACCATGGTTATTTTCAGGGACCGACTACCGGACTTg < 1:521621/71‑1 (MQ=255) aaaaCGGGTAAAACCTTGATGCTGGCTTTTGACCATGGTTATTTTCAGGGACCGACTACCGAACTTGAACg < 1:1249768/71‑1 (MQ=255) aaaCCGTGATGCTGGCTTTTGACCATGGTTATTTTCAGGGACCGACTACCGAACTTGAACGCATTGAtata < 1:195109/71‑1 (MQ=255) aaaCCGTGATGCTGGCTTTTGACCATGGTTATTTTCAGGGACCGACTACCGAACTTGAACGCATTGAtata < 1:2617463/71‑1 (MQ=255) aaCCGTGATGCTGGCTTTTGACCATGGTTATTTTCAGGGACCGACTACCGAACTTGAACGCATTGAtata < 1:195112/70‑1 (MQ=255) ccGTGATGCTGGCTTTTGACCATGGTTATTTTCAGGGACCGACTACCGGACTTGAACGCATTGATATAAat < 1:746364/71‑1 (MQ=255) cTGGCTTTTGACCATGGTTATTTTCAGGGACCGACTACCGGACTTGAACGCATTGATATAAATATCGcccc > 1:2018107/1‑71 (MQ=255) ggCTTTTGACCATGGTTATTTTCAGGGACCGACTACCGAACTTGAACGCATTGATATAAATATCGCCCCGc > 1:1966555/1‑71 (MQ=255) cTTTTGACCATGGTTATTTTCAGGGACCGACTACCGGACTTGaa > 1:126723/1‑44 (MQ=255) cATGGTTATTTTCAGGGACCGACTACCGGACTTGAACg < 1:860513/38‑1 (MQ=255) cATGGTTATTTTCAGGGACCGACTACCGGACTTGAACGCATTGATATAAATATCGCCCCGCTGTTTGAACa < 1:138332/71‑1 (MQ=255) gTTATTTTCAGGGACCGACTACCGGACTTGAACGCATTGATATAAATATCGCCCCGCTGTTTGAACATGcc > 1:2115999/1‑71 (MQ=255) ttATTTTCAGGGACCGACTACCGAACTTGAACGCATTGAtata < 1:1559632/43‑1 (MQ=255) ttATTTTCAGGGACCGACTACCGAACTTGAACGCATTGAtata < 1:925889/43‑1 (MQ=255) tATTTTCAGGGACCGACTACCGAACTTGAACGCAATGATATAAATATCGCCCCGCTGTTTGAAc > 1:530398/1‑64 (MQ=255) aGGGACCGACTACCGGACTTGAACGCATTGATATAAATATCGCCCCGCTGTTTGAACATGCCGATGTAtt < 1:2254895/70‑1 (MQ=255) aGGGACCGACTACCGGACTTGAACGCATTGATATAAATATCGCCCCGCTGTTTGAACATGCCGATGTAtt < 1:1022517/70‑1 (MQ=255) aGGGACCGACTACCGGACTTGAACGCATTGATATAAATATCGCCCCGCTGTTTGAACATGCCGATGTAtt < 1:156525/70‑1 (MQ=255) aCCGACTACCGGACTTGAACGCATTGATATAAATATCGCCCCGCTGTTTGAACATGCCGATGTATTAAtgt > 1:2654449/1‑71 (MQ=255) aCTACCGGACTTGAACGCATTGATATAAATATCGCCCCGCTGTTTGAAc < 1:721982/49‑1 (MQ=255) ccGAACTTGAACGCATTGATATAAATATCGCCCCGCTGTTTGAACa > 1:2603746/1‑46 (MQ=255) gACTTGAACGCATTGATATAAATATCGCCCCGCTGTTTGAACa > 1:2170448/1‑43 (MQ=255) gACTTGAACGCATTGATATAAATATCGCCCCGCTGTTTGAACa > 1:1841700/1‑43 (MQ=255) | TCCGAAAACGGGTAAAACCGTGATGCTGGCTTTTGACCATGGTTATTTTCAGGGACCGACTACCGGACTTGAACGCATTGATATAAATATCGCCCCGCTGTTTGAACATGCCGATGTATTAATGT > W3110S.gb/1607948‑1608072 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |