| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,609,975 | C→T | 68.8% | L254L (CTG→CTA) | yneE ← | conserved inner membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,609,975 | 0 | C | T | 68.8% | 58.2 / 39.6 | 48 | L254L (CTG→CTA) | yneE | conserved inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base C (7/8); new base T (24/9); total (31/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.08e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CAGATGGCATCCAGTGGTAAATCATTGTTTTCAGTACCGAACGGATCTTCCAGTTCTTCCGCCAGACAATCCAGCGAAATAAAAGTGTAGGAAATCAGCACAGAGATAAAAGGCGTCATGTAATGC > W3110S.gb/1609913‑1610038 | cAGATGGCATCCAGTGGTAAATCATTGTTTTCAGTACCGAACGGATCTTCCAGTTCTTCCGCCAGACAAt < 1:1006762/70‑1 (MQ=255) ggCATCCAGTGGTAAATCATTGTTTTCAGTACCGAACGGATCTTCCAGTTCTTCCGCTAGACAATCCAGc > 1:2415820/1‑70 (MQ=255) ggCATCCAGTGGTAAATCATTGTTTTCAGTACCGAACGGATCTTCCAGTTCTTCCGCTAGACAATCCAGc > 1:97375/1‑70 (MQ=255) ggCATCCAGTGGTAAATCATTGTTTTCAGTACCGAACGGATCTTCCAGTTCTTCCGCTAGACAATCCAGc > 1:577400/1‑70 (MQ=255) ggCATCCAGTGGTAAATCATTGTTTTCAGTACCGAACGGATCTTCCAGTTCTTCCGCTAGACAATCCAGc > 1:463759/1‑70 (MQ=255) ggCATCCAGTGGTAAATCATTGTTTTCAGTACCGAACGGATCTTCCAGTTCTTCCGCTAGACAATCCAGc > 1:384671/1‑70 (MQ=255) gCATCCAGTGGTAAATCATTGTTTTCAGTACCGAACGGATCTTCCAGTTCTTCCGCTAGACAATCCAGCGa < 1:1442393/71‑1 (MQ=255) gCATCCAGTGGTAAATCATTGTTTTCAGTACCGAACGGATCTTCCAGTTCTTCCGCTAGACAATCCAGCGa < 1:2200692/71‑1 (MQ=255) cAGTGGTAAATCATTGTTTTCAGTACCGAACGGATCTTCCAGTTCTTCCGCTAGACAATCCAg > 1:1633098/1‑63 (MQ=255) cAGTGGTAAATCATTGTTTTCAGTACCGAACGGATCTTCCAGTTCTTCCGCTAGACAATCCAg > 1:1636362/1‑63 (MQ=255) cAGTGGTAAATCATTGTTTTCAGTACCGAACGGATCTTCCAGTTCTTCCGCTAGACAATCCAg > 1:901588/1‑63 (MQ=255) cAGTGGTAAATCATTGTTTTCAGTACCGAACGGATCTTCCAGTTCTTCCGCTAGACAATCCAg > 1:1848313/1‑63 (MQ=255) cAGTGGTAAATCATTGTTTTCAGTACCGAACGGATCTTCCAGTTCTTCCGCTAGACAATCCAGCGAAATa > 1:1932563/1‑70 (MQ=255) ggTAAATCATTGTTTTCAGTACCGAACGGATCTTCCAGTTCTTCCGCTAGACAATCCAGCGAAATAAAAg > 1:450381/1‑70 (MQ=255) gTAAATCATTGTTTTCAGTACCGAACGGATCTTCCAGTTCTTCCGCTAGACAATCCAGCGAAATAAAAgtg > 1:1760966/1‑71 (MQ=255) gTAAATCATTGTTTTCAGTACCGAACGGATCTTCCAGTTCTTCCGCTAGACAATCCAGCGAAATAAAAgtg > 1:210858/1‑71 (MQ=255) gTAAATCATTGTTTTCAGTACCGAACGGATCTTCCAGTTCTTCCGCCAGACAATCCAGCGaaa > 1:113703/1‑63 (MQ=255) aaaTCATTGTTTTCAGTACCGAACGGATCTTCCAGTTCTTCCGCTAGACAATCCAGCGAAATAAAAgtg > 1:1607/1‑69 (MQ=255) aaTCATTGTTTTCAGTACCGAACGGATCTTCCAGTTCTTCCGCTAGACAATCCAGCTAAATAAAAgtgt < 1:2748962/69‑1 (MQ=255) aaTCATTGTTTTCAGTACCGAACGGATCTTCCAGTTCTTCCGCTAGACAATCCAGCGAAATAAAAGTGTAg > 1:2046262/1‑71 (MQ=255) aaTCATTGTTTTCAGTACCGAACGGATCTTCCAGTTCTTCCGCCAGACAATCCAGCGAAATAAAAGTGTAg > 1:451031/1‑71 (MQ=255) aaTCATTGTTTTCAGTACCGAACGGATCTTCCAGTTCTTCCGCCAGACAATCCAGCGAAATAAAAGTGTAg > 1:447794/1‑71 (MQ=255) aTCATTGTTTTCAGTACCGAACGGATCTTCCAGTTCTTCCGCCAGACAATc < 1:45886/51‑1 (MQ=255) tCATTGTTTTCAGTACCGAACGGATCTTCCAGTTCTTCCGCTAGACAATCCAGCGAAATAAAAGTGTAGGa < 1:2517906/71‑1 (MQ=255) cATTGTTTTCAGTACCGAACGGATCTTCCAGTTCTTCCGCCAGACAATCCAGCGAAATAAAAGTGTAGGa > 1:1794498/1‑70 (MQ=255) cATTGTTTTCAGGACCGAACGGATCTTCCAGTTCTTCCGCTAGa > 1:2704449/1‑44 (MQ=38) aTTGTTTTCAGTACCGAACGGATCTTCCAGTTCTTCCGCCAGACAATCCAGCGAAATAAAAg > 1:942296/1‑62 (MQ=255) aTTGTTTTCAGTACCGAACGGATCTTCCAGTTCTTCCGCCAGACAATCCAGCGAAATAAAAg > 1:2689900/1‑62 (MQ=255) gTTTTCAGTACCGAACGGATCTTCCAGTTCTTCCGCTAGACAATCCAGCGAAATAAAAGTGTAGGAAATc < 1:2126641/70‑1 (MQ=255) gTTTTCAGTACCGAACGGATCTTCCAGTTCTTCCGCTAGACAATCAAGCGAAATAAAAGAGTa > 1:568794/1‑63 (MQ=255) ttttCAGTACCGAACGGATCTTCCAGTTCTTCCGCCAGAc < 1:1788412/40‑1 (MQ=255) ttCAGTACCGAACGGATCTTCCAGTTCTTCCGCTAGACAATCCAg > 1:1225698/1‑45 (MQ=255) ccGAACGGATCTTCCAGTTCTTCCGCCAGACAATCCAGCGAAATaaaa > 1:2159518/1‑48 (MQ=255) aaCGGATCTTCCAGTTCTTCCGCCAGACAATCCAGCGAAATAAAAgtgt < 1:1901012/49‑1 (MQ=255) aaCGGATCTTCCAGTTCTTCCGCCAGACAATCCAGCGAAATAAAAgtgt < 1:897929/49‑1 (MQ=255) aCGGATCTTCCAGTTCTTCCGCCAGACAATCCAGCGAAATAAAAGTGTAGGAAATCAGCACAGAGATaaaa < 1:972930/71‑1 (MQ=255) gATCTTCCAGTTCTTCCTCTAGACAATCCAGCGAAATaa < 1:2354807/39‑1 (MQ=37) aTCTTCCAGTTCTTCCGCTAGACAATCCAGCGAAATAAAAGTGTAGGAAATCAGCACAGAGATaaaa < 1:2806193/67‑1 (MQ=255) ccAGTTCTTCCGCCAGACAATCCAGCGAAATAAAAGTGTAGGAAATCAGCACAGAGATAAAAGGCGt < 1:2273069/67‑1 (MQ=255) ccAGTTCTTCCGCCAGACAATCCAGCGAAATAAAAGTGTAGGAAATCAGCACAGAGATAAAAGGCGt < 1:1223702/67‑1 (MQ=255) ttcttcCGCTAGACAATCCAGCGAAATAAAAGTGTAGGAAATCAGCACAGAGATaa < 1:253035/56‑1 (MQ=255) ttcttcCGCTAGACAATCCAGCGAAATAAAAGTGTAGGAAATCAGCACAGAGATAAAAGGCGTCATGTa > 1:502612/1‑69 (MQ=255) tcttcCGCTAGACAATCCAGCGAAATAAAAGTGTa > 1:2443588/1‑35 (MQ=255) cttcCGCTAGACAATCCAGCGAAATAAAAGTGTAGGAAATCAGCACAGAGATaaaa > 1:2626942/1‑56 (MQ=255) cttcCGCTAGACAATCCAGCGAAATAAAAGTGTAGGAAATCAGCACAGAGATAAAAGGCGTCATGTAATg > 1:2307182/1‑70 (MQ=255) cttcCGCTAGACAATCCAGCGAAATAAAAGTGTAGGAAATCAGCACAGAGATAAAAGGCGTCATGTAATg > 1:134618/1‑70 (MQ=255) cttcCGCTAGACAATCCAGCGAAATAAAAGTGTAGGAAATCAGCACAGAGATAAAAGGCGTCATGTAATg > 1:1746544/1‑70 (MQ=255) ccGCTAGACAATCCAGCGAAATAAAAGTGTAGGAAATCAGCACAGAGATAAAAGGCGTCATGTAATGc < 1:328324/68‑1 (MQ=255) | CAGATGGCATCCAGTGGTAAATCATTGTTTTCAGTACCGAACGGATCTTCCAGTTCTTCCGCCAGACAATCCAGCGAAATAAAAGTGTAGGAAATCAGCACAGAGATAAAAGGCGTCATGTAATGC > W3110S.gb/1609913‑1610038 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |