| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,621,163 | A→G | 12.9% | Q110Q (CAA→CAG) | marR → | DNA‑binding transcriptional repressor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,621,163 | 0 | A | G | 12.9% | 71.5 / 5.1 | 31 | Q110Q (CAA→CAG) | marR | DNA‑binding transcriptional repressor |
| Reads supporting (aligned to +/- strand): ref base A (16/11); new base G (2/2); total (18/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.62e-01 | |||||||||||
CCCGAATGACAAGCGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAATATGTGAACAATGCCATCAATTAGTTGGCCAGGACCTGCACCAAGAATTAACAAAAAACCTGACGGCGGACGAAGTGG > W3110S.gb/1621100‑1621230 | cccGAATGACAAGCGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAATATGTGAACAATGCCAt > 1:2311622/1‑70 (MQ=255) gAATGACAAGCGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAATATGTGAACAATGCCATCAAt < 1:2267933/71‑1 (MQ=255) aTGACAAGCGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAATATGTGAACAATGCCATCAATTa < 1:302045/71‑1 (MQ=255) aCAAGCGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAATATGTGAACAATGCCATCAATTAGtt > 1:2774926/1‑71 (MQ=255) aCAAGCGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAATATGTGAACAATGCCATCAATTAGtt > 1:2576427/1‑71 (MQ=255) cAAGCGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAATATGTGAACAATGCCATCAATTAGTTg > 1:514244/1‑71 (MQ=255) gTACTGGTAAAACTTACCACCGGCGGCGCGGCAATATGTGAACAATGCCATCAATTAGTTGGCCAGGACCt > 1:261744/1‑71 (MQ=255) tACTGGTAAAACTTACCACCGGCGGCGCGGCAATATGTGAACAATGCCATCAATTa < 1:84449/56‑1 (MQ=255) tACTGGTAAAACTTACCACCGGCGGCGCGGCAATATGTGAACAATGCCATCAATTAGTTGGcc < 1:626374/63‑1 (MQ=255) cTGGTAAAACTTACCACCGGCGGCGCGGCAATATGTGAACAATGCCATCAATTAGTTGGCCAGGACCTGCa > 1:1735967/1‑71 (MQ=255) ggTAAAACTTACCACCGGCGGCGCGGCAATATGTGAACAATGCCATCAATTAGTTGGCCAGGACCTGCAc > 1:1053997/1‑70 (MQ=255) gTAAAACTTACCACCGGCGGCGCGGCAATATGTGAACAGTGCCATCAATTAGTTGGc > 1:232614/1‑57 (MQ=255) gTAAAACTTACCACCGGCGGCGCGGCAATATGTGAACAATGCCATCAATTAGTTGGCCAGGACCTGCAcc > 1:117774/1‑70 (MQ=255) aaaaCTTACCACCGGCGGCGCGGCAATATGTGAACaa > 1:2331299/1‑37 (MQ=255) aaaCTTACCACCGGCGGCGCGGCAATATGTGAACAATGCCATCAATTAGTTGGCCAGGACCTGCACCAAg > 1:2262725/1‑70 (MQ=255) aaCTTACCACCGGCGGCGCGGCAATATGTGAACAATGCCATCAATTAGTTGGcc < 1:781891/54‑1 (MQ=255) aaCTTACCACCGGCGGCGCGGCAATATGTGAACAATGCCATCAATTAGTTGGcc < 1:2719136/54‑1 (MQ=255) cTTACCACCGGCGGCGCGGCAATATGTGAACAATGCCa > 1:2637712/1‑38 (MQ=255) cTTACCACCGGCGGCGCGGCAATATGTGAACAATGCCATCAATTAGTTGGCCAGGACCTGCACCAAGAAtt < 1:2387855/71‑1 (MQ=255) tACCACCGGCGGCGCGGCAATATGTGAACAATGCCATCAATTAGTTGGCCAGGACCTGCACCAAGAATTaa > 1:671374/1‑71 (MQ=255) ccGGCTGCGCGGCAATATGTGAACAGTGCCATCAATTAGTTGGCCAgg < 1:1155141/48‑1 (MQ=255) ccGGCGGCGCGGCAATATGTGAACAGTGCCATCAATTAGTTGGCCAgg < 1:1673354/48‑1 (MQ=255) ccGGCGGCGCGGCAATATGTGAACAATGCCATCAATTAGTTGGCCAGGACCTGCACCAAGAATTAACaaa > 1:2553015/1‑70 (MQ=255) ggcggcGCGGCAATATGTGAACAATGCCATCAATTAGTTGGCCAGGACCTGCACCAAGAATTAACaaaaaa < 1:2382362/71‑1 (MQ=255) aaTATGTGAACAATGCCATCAATTAGTTGGCCAGGACCTGCACCAAGAATTAACAAAAAACCTGAcggcgg < 1:404158/71‑1 (MQ=255) tatGTGAACAATGCCATCAATTAGTTGGCCAGGACCTGCACCAAGAATTAACAAAAAACCTGAcggcg < 1:677474/68‑1 (MQ=255) tgtgAACAGTGCCATCAATTAGTTGGCCAGGACCTGCACCAAGAATTAACAAAAAACCTGACGGCGGACg > 1:257971/1‑70 (MQ=255) tgtgAACAATGCCATCAATTAGTTGGCCAGGACCTGCACCAAGAATTAACAAAAAACCTGACGGCGGACg > 1:1878272/1‑70 (MQ=255) tgtgAACAATGCCATCAATTAGTTGGCCAGGACCTGCACCAAGAATTAACAAAAAACCTGACGGCGGACGa < 1:926929/71‑1 (MQ=255) gtgAACAATGCCATCAATTAGTTGGCCAGGACCTGCACCAAGAATTAACAAAAAACCTGa > 1:630141/1‑60 (MQ=255) tgAACAATGCCATCAATTAGTTGGCCAGGACCTGCACCAAGAATTAACAAAAAACCTGACGGCGGACGAAg > 1:1701422/1‑71 (MQ=255) cAATGCCATCAATTAGTTGGCCAGGACCTGCACCAAGAATTAACAAAAAACCTGACGGCGGACGAAGTgg > 1:1796883/1‑70 (MQ=255) | CCCGAATGACAAGCGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAATATGTGAACAATGCCATCAATTAGTTGGCCAGGACCTGCACCAAGAATTAACAAAAAACCTGACGGCGGACGAAGTGG > W3110S.gb/1621100‑1621230 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |