| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,649,687 | T→C | 19.3% | Y14H (TAT→CAT) | dicA → | predicted regulator for DicB |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,649,687 | 0 | T | C | 19.3% | 59.8 / 8.7 | 29 | Y14H (TAT→CAT) | dicA | predicted regulator for DicB |
| Reads supporting (aligned to +/- strand): ref base T (13/10); new base C (2/3); total (15/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.39e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.85e-01 | |||||||||||
GTCAATACGATTTAGCATTAGCTAACTATGGAAACAAAAAATTTAACTATCGGCGAACGCATCAGGTATCGTCGGAAAAACCTCAAACACACCCAAAGGTCTCTTGCTAAAGCCCTGAAAATCTCCCATGTGTCTG > W3110S.gb/1649621‑1649756 | gTCAATACGATTTAGCATTAGCTAACTATGGAAACAAAAAATTTAACTATCGGCGAACGCATCAGGTAtc < 1:553749/70‑1 (MQ=255) cGATTTAGCATTAGCTAACTATGGAAACAAAAAATTTAACTATCGGCGAACGCATCAGGTATCGTCGGaaa < 1:617289/71‑1 (MQ=255) cGATTTAGCATTAGCTAACTATGGAAACAAAAAATTTAACTATCGGCGAACGCATCAGGTATCGTCGGaaa < 1:495112/71‑1 (MQ=255) gATTTAGCATTAGCTAACTATGGAAACAAAAAATTTAACTTTCGGCGAACGCATCAGGTATCGTCGGaaaa < 1:299118/71‑1 (MQ=255) aTTAGCTAACTATGGAAACAAAAAATTTAACTATCGGCGAACGCATCAGGTATCGTCGGAAAAACCTCaaa < 1:877731/71‑1 (MQ=255) ttAGCTAACTATGGAAACAAAAAATTTAACTATCGGCGAACGCATCAGGTATCGTCGGAAAAACCTCa < 1:1853836/68‑1 (MQ=255) aGCTAACTATGGAAACAAAAAATTTAACTATCGGCGAACGCATCAGGTATCGTCGGAAAAACCTCAAacac > 1:2290456/1‑71 (MQ=255) aGCTAACTATGGAAACAAAAAATTTAACTATCGGCGAACGCATCAGGTATCGTCGGAAAAACCTCAAaca > 1:1046486/1‑70 (MQ=255) aGCTAACTATGGAAACAAAAAATTTAACTATCGGCGAACGCATCAGGTATCGTCGGAAAAACCTCAAaca > 1:434914/1‑70 (MQ=255) aGCTAACTATGGAAACAAAAAATTTAACTATCGGCGAACGCATCAGGTATCGTCGGAAAAACCTCAAaca > 1:2008907/1‑70 (MQ=255) aaCTATGGAAACAAAAAATTTAACTATCGGCGAACGCATCAGGTAt > 1:299413/1‑46 (MQ=255) aTGGAAACAAAAAATTTAACTATCGGCGAACGCATCAGGCATCGTCGGaaa < 1:1859500/51‑1 (MQ=255) aTGGAAACAAAAAATTTAACTATCGGCGAACGCATCAGGCATCGTCGGaaa < 1:2271234/51‑1 (MQ=255) aTGGAAACAAAAAATTTAACTATCGGCGAACGCATCAGGCATCGTCGGAAAAACCTCAAaca > 1:1194018/1‑62 (MQ=255) aTGGAAACAAAAAATTTAACTATCGGCGAACGCATCAGGAATCGTCGGaaa < 1:935178/51‑1 (MQ=255) tGGAAACAAAAAATTTAACTATCGGCGAACGCATCAGGt < 1:1538053/39‑1 (MQ=255) tGGAAACAAAAAATTTAACTATCGGCGAACGCATCAGGTATCGTCGGAAAAACCTCAAACACACCCAAAg < 1:537341/70‑1 (MQ=255) ggAAACAAAAAATTTAACTATCGGCGAACGCATCAGGTATCGTCGGAAAAACCTCAAacacac > 1:2167808/1‑63 (MQ=255) ggAAACAAAAAATTTAACTATCGGCGAACGCATCAGGTATCGTCGGAAAAACCTCAAacacac > 1:1570518/1‑63 (MQ=255) tttAACTATCGGCGAACGCATCAGGCATCGTCGGaaaa < 1:477983/38‑1 (MQ=255) tCGGCGAACGCATCAGGTATCGTCGGAAAAACCTCAAACACACCCAAAGGTCTCTTGCTAAAGCCCTGaa < 1:395883/70‑1 (MQ=255) ggCGAACGCATCAGGCATCGTCGGAAAAACCTCAAACACACCCAAAGGTCTCTTGCTAAAGCCCTGAAAAt > 1:2138557/1‑71 (MQ=255) aCGCATCAGGTATCGTCGGAAAAACCTCAAACACACCCAAAGGTCTCTTGCTAAAGCCCTGAAAATCTcc < 1:148720/70‑1 (MQ=255) aGGTATCGTCGGAAAAACCTCAAACACACCCAAAg > 1:1717513/1‑35 (MQ=255) aGGTATCGTCGGAAAAACCTCAAACACACCCAAAg > 1:311300/1‑35 (MQ=255) aGGTATCGTCGGAAAAACCTCAAACACACCCAAAGGtctc > 1:1531909/1‑40 (MQ=255) aGGTATCGTCGGAAAAACCTCAAACACACCCAAAGGTctct > 1:358078/1‑41 (MQ=255) aGGTATCGTCGGAAAAACCTCAAACACACCCAAAGGTctct > 1:1552347/1‑41 (MQ=255) aGGTATCGTCGGAAAAACCTCAAACACACCCAAAGGTCTCTTGCTAAAGCCCTGAAAATCTCCCATGTGTc > 1:2156724/1‑71 (MQ=255) gTATCGTCGGAAAAACCTCAAACACACCCAAAGGTCTCTTGCTAAAGCCCTGAAAATCTCCCATGTGTCTg < 1:1207858/71‑1 (MQ=255) | GTCAATACGATTTAGCATTAGCTAACTATGGAAACAAAAAATTTAACTATCGGCGAACGCATCAGGTATCGTCGGAAAAACCTCAAACACACCCAAAGGTCTCTTGCTAAAGCCCTGAAAATCTCCCATGTGTCTG > W3110S.gb/1649621‑1649756 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |