| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 237,726 | A→G | 14.9% | N131S (AAC→AGC) | yafT → | predicted aminopeptidase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 237,726 | 0 | A | G | 14.9% | 113.6 / 12.8 | 47 | N131S (AAC→AGC) | yafT | predicted aminopeptidase |
| Reads supporting (aligned to +/- strand): ref base A (16/24); new base G (2/5); total (18/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.92e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CATTCTCTGGTATCCCGGACAGGCAAAAGCCTCTGACTTGTAACAAAAACAAAGATAAAAATGAGAACGAGGATGTTGCCAGTGCTGAGAATATGAACTGGATGCAGGCACTGCGTTTTGTGGCTGCAAAAGGA > W3110S.gb/237660‑237793 | caTTCTCTGGTATCCCGGACAGGCAAAAGCCTCTGACTTGTAACAAAAACAAAGATAAAAATGAGAACGAg < 1:283958/71‑1 (MQ=255)caTTCTCTGGTATCCCGGACAGGCAAAAGCCTCTGACTTGTAACAAAAACAAAGATAAAAATGAGAACGAg < 1:2806515/71‑1 (MQ=255)caTTCTCTGGTATCCCGGACAGGCAAAAGCCTCTGACTTGTAACAAAAACAAAGATAAAAATGAGAACGAg < 1:1844808/71‑1 (MQ=255) aTTCTCTGGTATCCCGGACAGGCAAAAGCCTCTGACTTGTAACAAAAACAAAGATAAAAATGAGAACGAgg < 1:2804044/71‑1 (MQ=255) aTTCTCTGGTATCCCGGACAGGCAAAAGCCTCTGACTTGTAACAAAAACAAAGATAAAAATGAGAACGAg > 1:916376/1‑70 (MQ=255) aTTCTCTGGTATCCCGGACAGGCAAAAGCCTCTGACTTGTAACAAAAACAAAGATAAAAATGAGAACGAg > 1:1209769/1‑70 (MQ=255) tctcTGGTATCCCGGACAGGCAAAAGCCTCTGACTTGTAACAAAAACAAAGATAAAAATGAGAACGAGGa < 1:1160097/70‑1 (MQ=255) gTATCCCGGACAGGCAAAAGCCTCTGACTTGTAACAAAAACAAAGATAAAAATGAGAGCGAGGATGTTGc > 1:2428102/1‑70 (MQ=255) gTATCCCGGACAGGCAAAAGCCTCTGACTTGTAACAAAAACAAAGATAAAAATGAGAACGAGGATGTTGcc < 1:816345/71‑1 (MQ=255) gTATCCCGGACAGGCAAAAGCCTCTGACTTGTAACAAAAACAAAGATAAAAATGAGAACGAGGATGTTGcc < 1:2297572/71‑1 (MQ=255) gTATCCCGGACAGGCAAAAGCCTCTGACTTGTAACAAAAACAAAGATAAAAATGAGAACGAGGATGTTGc < 1:1965124/70‑1 (MQ=255) ccGGACAGGCAAAAGCCTCTGACTTGTAACAAAAACAAAGATAAAAATGAGAACGAGGATGTTGCCa > 1:2207219/1‑67 (MQ=255) ccGGACAGGCAAAAGCCTCTGACTTGTAACAAAAACAAAGATAAAAATGAGAACGAGGATGTTGCCAGTg > 1:2779688/1‑70 (MQ=255) aGGCAAAAGCCTCTGACTTGTAACAAAAACAAAGATAAAAATGAGAACGAGGATGTTGCCAGTGCTGAGaa < 1:1545747/71‑1 (MQ=255) gCAAAAGCCTCTGACTTGTAACAAAAACAAAGATAAAAATGAGAACGAGGATGTTGCCAg < 1:957867/60‑1 (MQ=255) gCAAAAGCCTCTGACTTGTAACAAAAACAAAGATAAAAATGAGAACGAGGATGTTGCCAGTGCTGAGAata > 1:1045636/1‑71 (MQ=255) cAAAAGCCTCTGACTTGTAACAAAAACAAAGATAAAAATGAGAACGAGGATGTTGCCAg > 1:123968/1‑59 (MQ=255) cAAAAGCCTCTGACTTGTAACAAAAACAAAGATAAAAATGAGAACGAGGATGTTGCCAGTGCTGAGAatat > 1:2734779/1‑71 (MQ=255) aaaaGCCTCTGACTTGTAACAAAAACAAAGATAAAAATGAGAACGAGGATGTTGCCAGTGCTGAGAatat > 1:1180324/1‑70 (MQ=255) aaaaGCCTCTGACTTGTAACAAAAACAAAGATAAAAATGAGAACGAGGATGTTGCCAGTGCTGAGAATATg < 1:2504538/71‑1 (MQ=255) aaaGCCTCTGACTTGTAACAAAAACAAAGATAAAAATGAGAGCGAGGATGTTGCCAGTGCTGAGaa < 1:2581505/66‑1 (MQ=255) aaaGCCTCTGACTTGTAACAAAAACAAAGATAAAAATGAGAGCGAGGATGTTGCCAGTGCTGAGaa < 1:1859061/66‑1 (MQ=255) aaaGCCTCTGACTTGTAACAAAAACAAAGATAAAAATGAGAACGAGGATGTTGCCAGTGCTGAGAATATGa < 1:1926319/71‑1 (MQ=255) ccTCTGACTTGTAACAAAAACAAAGATAAAAATGAGAACGAGGATGTTGCCAGTGCTGAGAATATGAACTg < 1:1278605/71‑1 (MQ=255) tctGACTTGTAACAAAAACAAAGATAAAAATGAGAACGAg < 1:320647/40‑1 (MQ=255) tGACTTGTAACAAAAACAAAGATAAAAATGAGAGCGAGGATGTTGCCAGTGCTGAGAATATGAAc > 1:643104/1‑65 (MQ=255) tGACTTGTAACAAAAACAAAGATAAAAATGAGAACGAGGATGtt > 1:1325297/1‑44 (MQ=255) tGACTTGTAACAAAAACAAAGATAAAAATGAGAACGAGGATGTTGCCAGTGCTGAGAATATGAACTGGATg > 1:2150688/1‑71 (MQ=255) tGACTTGTAACAAAAACAAAGATAAAAATGAGAACGAGGATGTTGCCAGTGCTGAGAATATGAACTGGATg > 1:2801193/1‑71 (MQ=255) tGACTTGTAACAAAAACAAAGATAAAAATGAGAACGAGGATGTTGCCAGTGCTGAGAATATGAACTGGATg > 1:1368582/1‑71 (MQ=255) ttGTAACAAAAACAAAGATAAAAATGAGAACGAGGATGTTGCCAGTGCTGAGAATATg < 1:65545/58‑1 (MQ=255) gTAACAAAAACAAAGATAAAAATGAGAACGAGGATGTTGCCAGTGCTGAGAATATGAACTGGATGCAgg < 1:2182565/69‑1 (MQ=255) gTAACAAAAACAAAGATAAAAATGAGAACGAGGATGTTGCCAGTGCTGAGAATATGAACTGGATGCAgg < 1:193325/69‑1 (MQ=255) gTAACAAAAACAAAGATAAAAATGAGAACGAGGATGTTGACAGTg > 1:540107/1‑45 (MQ=255) acaaaaacaaaGATAAAAATGAGAACGAGGATGTTGCCAGTGCTGAGAATATGAACTGGATGCAGGc < 1:1276810/67‑1 (MQ=255) caaaaacaaaGATAAAAATGAGAGCGAGGATGTTGCCAGTGCTGAGAATATg < 1:738076/52‑1 (MQ=255) caaaaacaaaGATAAAAATGAGAACGAGGATGTTGCCAGTGCTGAGAATATGaa > 1:1972718/1‑54 (MQ=255) caaaGATAAAAATGAGAACGAGGATGTTGCCAGTGCTGAGAATATGAACTGGa > 1:2054235/1‑53 (MQ=255) tAAAAATGAGAACGAGGATGTTGCCAGTGCTGAGAATATg < 1:1325750/40‑1 (MQ=255) aaaaTGAGAACGAGGATGTTGCCAGTGCTGAGAATATGAAc > 1:240954/1‑41 (MQ=255) aaaTGAGAGCGAGGATGTTGCCACTGCTGAGAatatt < 1:1930755/37‑2 (MQ=37) aaTGAGAACGAGGATGTTGCCAGTGCTGAGAATATGAACTg < 1:2658736/41‑1 (MQ=255) aaTGAGAACGAGGATGTTGCCAGTGCTGAGAATATGAACTg < 1:2537600/41‑1 (MQ=255) tGAGAGCGAGGATGTTGCCAGTGCTGAGAATATGAACTGGATGCAGGCACTGCGTTTTGTGGCTGCAAAAg < 1:1433266/71‑1 (MQ=255) tGAGAACGAGGATGTTGCCAGTGCTGAGAATATGAACTg < 1:883633/39‑1 (MQ=255) tGAGAACGAGGATGTTGCCAGTGCTGAGAATATGAACTg < 1:2060600/39‑1 (MQ=255) agaACGAGGATGTTGCCAGTGCTGAGAATATGAACTGGATGCAGGCACTGCGTTTTGTGGCTGCAAAAGGa < 1:1312353/71‑1 (MQ=255) aaCGAGGATGTTGCCAGTGCTGAGAATATGAACTGGATGCAgg < 1:30972/43‑1 (MQ=255) | CATTCTCTGGTATCCCGGACAGGCAAAAGCCTCTGACTTGTAACAAAAACAAAGATAAAAATGAGAACGAGGATGTTGCCAGTGCTGAGAATATGAACTGGATGCAGGCACTGCGTTTTGTGGCTGCAAAAGGA > W3110S.gb/237660‑237793 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |