Predicted mutation
evidence seq id position mutation freq annotation gene description
RA W3110S.gb 1,695,171 T→C 57.1% V269V (GTA→GTG)  uidB ← glucuronide transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb1,695,1710TC57.1% 1.4 / 13.2 14V269V (GTA→GTG) uidBglucuronide transporter
Reads supporting (aligned to +/- strand):  ref base T (2/4);  new base C (6/2);  total (8/6)
Fisher's exact test for biased strand distribution p-value = 2.77e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.98e-01

ACCATCCCCGGCACCAGCGGTGCCGATGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACATAGCGCACGTAGAACAACGACGAGGCGCT  >  W3110S.gb/1695115‑1695236
                                                        |                                                                 
aCCATCCCCGGCACCAGCGGTGCCGATGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGa                                                      <  1:1851666/70‑1 (MQ=255)
    tCCCCGGCACCAGCGGTGCCGATGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTg                                                       <  1:1721284/65‑1 (MQ=255)
             ccAGCGGTGCCGATGCCACAGTACCAACCAGGTTTTGCACCAGCACCAGCACAGTGAACAGCCCGGTAt                                          <  1:1456791/69‑1 (MQ=255)
                gCGGTGCCGATGCCACAGTACCAACCAGGTTTTGCACCAGCACCAGCACAGTGAACAGCCCGGTATCAttt                                     <  1:406977/71‑1 (MQ=255)
                    tGCCGATGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAaca                                 <  1:1805202/71‑1 (MQ=255)
                           gCCACAGTACCAACCAGGTTTTGCACCAGCACCAGCACAGTGAACAGCCCGGTATCATTTAACACATAgcg                          >  1:2804993/1‑71 (MQ=255)
                                aGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACATAGCGCACg                      >  1:627344/1‑70 (MQ=255)
                                 gTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAg                                                  >  1:798737/1‑41 (MQ=255)
                                    ccaaccaGGTTTTGCACCAGCACCAGCACAGTGAACAGCCCGGTATCATTTAACACATAGCGCACGTAGaa                 >  1:1767831/1‑71 (MQ=255)
                                    ccaaccaGGTTTTGCACCAGCACCAGCACAGTGAACAGCCCGGTATCATTTAACACATAGCGCACGTAGaa                 >  1:1871856/1‑71 (MQ=255)
                                          aGGTTTTGCACCAGCACCAGCACAGTGAACAGCCCGGTATCATTTAACACATAGCGCa                        >  1:2174151/1‑58 (MQ=255)
                                           ggTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACATAGCGCACGTAGAACAacgac          <  1:2469381/71‑1 (MQ=255)
                                              tttGCACCAGCACCAGCACAGTGAACAGCCCGGTATCATTTAACACATAGCGCACGTAGAACAACGACGAg       >  1:48040/1‑71 (MQ=255)
                                              tttGAACCAGCACCAGCACAGTGAACAGCCCGGTATCATTTAACACATAGCGCACGTAGAACAACgaggag       >  1:2532566/1‑71 (MQ=255)
                                                    ccagcaccagcaCAGTGAACAGCCCGGTATCATTTAACACATAGCGCACGTAGAACAacga           >  1:1868407/1‑61 (MQ=255)
                                                    ccagcaccagcaCAGTGAACAGCCCGGTATCATCTAACACATAGCGCACGTAGAACAACGACGAGGCGCt  >  1:2095787/1‑70 (MQ=255)
                                                        |                                                                 
ACCATCCCCGGCACCAGCGGTGCCGATGCCACAGTACCAACCAGGTTTTGCACCAGTACCAGCACAGTGAACAGCCCGGTATCATTTAACACATAGCGCACGTAGAACAACGACGAGGCGCT  >  W3110S.gb/1695115‑1695236

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑