| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,799,263 | G→A | 31.4% | S132S (AGC→AGT) | pheT ← | phenylalanine tRNA synthetase, beta subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,799,263 | 0 | G | A | 31.4% | 46.7 / 25.4 | 35 | S132S (AGC→AGT) | pheT | phenylalanine tRNA synthetase, beta subunit |
| Reads supporting (aligned to +/- strand): ref base G (12/12); new base A (3/8); total (15/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.81e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TCAAGTTTCAGGTATTCACGGATATCGGTGCCAATCGGCGCATCCGCAGGCAGTTCGATAATGCCGCTGTGATCGTCAGAAATGCCCAGTTCAGAGAAGGAGCACAGCATCCCTTCAGACGGTTCG > W3110S.gb/1799198‑1799323 | tcaAGTTTCAGGTATTCACGGATATCGGTGCCAATCGGCGCATCCGCAGGCAGTTCGATAATGCCACtgtg < 1:2735693/71‑1 (MQ=255) caAGTTTCAGGTATTCACGGATATCGGTGCCAATCGGCGCATCCGCAGGCAGTTCGATAATGCCGCTGTGa > 1:1556758/1‑71 (MQ=255) aaGTTTCAGGTATTCACGGATATCGGTGCCAATCGGCGCATCCGCAGGCAGTTCGATAATGCCGCTGTGa > 1:1950340/1‑70 (MQ=255) aaGTTTCAGGTATTCACGGATATCGGTGCCAATCGGCGCATCCGCAGGCAGTTCGATAATGCCGCTGTGAt > 1:963218/1‑71 (MQ=255) aaGTTTAAGGTATTCACGGATATCGGTGCCAATCGGCGCATCCGCAGGCAGTTAGATAATGCCGCTGTGAt > 1:4083/1‑71 (MQ=255) ttCAGGTATTCACGGATATCGGTGCCAATCGGCGCATCCGCAGGCAGTTCGATAATGCCGCTGTGATCGt > 1:1448551/1‑70 (MQ=255) aGGTATTCACGGATATCGGTGCCAATCGGCGCATCCGCAGGCAGTTCGATAATGCCGCTGTGATCGTCAg < 1:1363595/70‑1 (MQ=255) aGGTATTCACGGATATCGGTGCCAATCGGCGCATCCGCAGGCAGTTCGATAATGCCACTGTGATCGTCAGa < 1:1288378/71‑1 (MQ=255) gTATTCACGGATATCGGTGCCAATCGGCGCATCCGCAGGCAGTTCGATAATGCCGCTGTGATCGTCAGaaa > 1:1430733/1‑71 (MQ=255) cACGGATATCGGTGCCAATCGGCGCATCCGCAGGCAGTTCGATAATGCCACTGTGATCGTCAGAAATGcc > 1:1848895/1‑70 (MQ=255) tCGGTGCCAATCGGCGCATCCGCAGGCAGTTCGATAATGCCGCTGTGATCGTCAGAAATGCCCAGTTCaga < 1:119274/71‑1 (MQ=255) tCGGTGCCAATCGGCGCATCCGCAGGCAGTTCGATAATGCCGCTGTGATCGTCAGAAATGCCCAGTTCag < 1:2470337/70‑1 (MQ=255) tCGGTGCCAATCGGCGCATCCGCAGGCAGTTCGATAATGCCGCTGTGATCGTCAGAAATGCCCAGTTCag < 1:530644/70‑1 (MQ=255) tCGGTGCCAATCGGCGCATCCGCAGGCAGTTCGATAATGCCACTGTGATCGTCAGAAATg > 1:1575565/1‑60 (MQ=255) ggTGCCAATCGGCGCATCCGCAGGCAGTTCGATAATGCCGCTGTGATCGTCAGAAATGCCCAGTTCagag < 1:59039/70‑1 (MQ=255) ggTGCCAATCGGCGCATCCGCAGGCAGTTCGATAATGCCACTGTGATCGTCAGAAATGCCCAGTTCagag < 1:1311289/70‑1 (MQ=255) tGCCAATCGGCGCATCCGCAGGCAGTTCGATAATGCCACTGTGATCGTCAGAAATGCCCAGTTCTGAGAAg < 1:959/71‑1 (MQ=255) tGCCAATCGGCGCATCCGCAGGCAGTTCGATAATGCCACTGTGATCGTCAGAAATGCCCAGTTCAGAGAAg < 1:627086/71‑1 (MQ=255) cAATCGGCGCATCCGCAGGCAGTTCGATAATGCCACTGTGATCGTCAGAAATGCCCAGTTCAGAGAAGGAg < 1:2000167/71‑1 (MQ=255) cAATCGGCGCATCCGCAGGCAGTTCGATAATGCCACTGTGATCGTCAGAAATGCCCAGTTCAGAGAAGGAg < 1:2734109/71‑1 (MQ=255) cAATCGGCGCATCCGCAGGCAGTTCGATAATGCCACTGTGATCGTCAGAAATGCCCAGTTCAGAGAAGGAg > 1:245007/1‑71 (MQ=255) cAATCGGCGCATCCGCAGGCAGTTCGATAATGCCACTGTGATCGTCAGAAATGCCCAGTTCAGAGAAGGAg < 1:191949/71‑1 (MQ=255) cATCCGCAGGCAGTTCGATAATGCCGCTGTGATCGTCAGAAATGCCCAGTTCAGAGAAGGAGCACAGCATc < 1:57090/71‑1 (MQ=255) tCCGCAGGCAGTTCGATAATGCCGCTGTGATCGTCAGAAATGCCCAGTTCag < 1:450078/52‑1 (MQ=255) caggcagTTCGATAATGCCGCTGTGATCGTCAGAAATGCCCAGTTCAGAGAAGGAGCACAGCATccc > 1:727266/1‑67 (MQ=255) caggcagTTCGATAATGCCGCTGTGATCGTCAGAAATGCCCAGTTCAGAGAAGGAGCACAGCATccc > 1:837203/1‑67 (MQ=255) ggcagTTCGATAATGCCGCTGTGATCGTCAGAAATGCCCAGTTCAGAGAAgg < 1:2343227/52‑1 (MQ=255) gcagTTCGATAATGCCGCTGTGATCGTCAGAAATGCCCAGTTCAGAGAAGGAGCACAGCATCCCTTCAGAc < 1:1419619/71‑1 (MQ=255) agTTCGATAATGCCGCTGTGATCGTCAGAAATGCCCAGTTCAGAGAAGGAGCACAGCAt < 1:2731065/59‑1 (MQ=255) agTTCGATAATGCCGCTGTGATCGTCAGAAATGCCCAGTTCAGAGAAGGAGCACAGCAt < 1:758001/59‑1 (MQ=255) agTTCGATAATGCCGCTGTGATCGTCAGAAATGCCCAGTTCAGAGAAGGAGCACAGCAt < 1:2037615/59‑1 (MQ=255) cGATAATGCCGCTGTGATCGTCAGAAATGCCCAGTTCAGAGAAGGAGCACAGCATCCCTTCAGACGGTTCg > 1:64815/1‑71 (MQ=255) cGATAATGCCGCTGTGATCGTCAGAAATGCCCAGTTCAGAGAAGGAGCACAGCATCCCTTCAGACGGTTCg > 1:727706/1‑71 (MQ=255) gCCGCTGTGATCGTCAGAAATGCCCAGTTCAGAGAAGGAGca > 1:2597228/1‑42 (MQ=255) ccGCTGTGATCGTCAGAAATGCCCAGTTCAGAGAAGGAGCACAGCATCCCTTCAGa > 1:35615/1‑56 (MQ=255) | TCAAGTTTCAGGTATTCACGGATATCGGTGCCAATCGGCGCATCCGCAGGCAGTTCGATAATGCCGCTGTGATCGTCAGAAATGCCCAGTTCAGAGAAGGAGCACAGCATCCCTTCAGACGGTTCG > W3110S.gb/1799198‑1799323 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |