| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,807,728 | C→T | 43.7% | A24T (GCT→ACT) | ydiY ← | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,807,728 | 0 | C | T | 43.7% | 10.0 / 38.1 | 32 | A24T (GCT→ACT) | ydiY | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base C (9/9); new base T (5/9); total (14/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.90e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.53e-01 | |||||||||||
TACCTTCGAAAGGTTTTTTAGCGCTGGCAGGATCATCCATGACAGTAAAAACGGAATCATCGGCAGCTGCATTCAGTGACGCAAACATGCCCCCCGCCAACATAACGATGGCAGGAACTGTCTTCAAA > W3110S.gb/1807662‑1807789 | tACCTTCGAAAGGTTTTTTAGCGCTGGCAGGATCATCCATGACAGTAAAAACGGAATCATCGGCAGCTGCa > 1:2495566/1‑71 (MQ=255)tACCTTCGAAAGGTTTTTTAGCGCTGGCAGGATCATCCATGACAGTAAAAACGGAATCATCGGCAGCTGCa > 1:2146157/1‑71 (MQ=255) gTTTTTTAGCGCTGGCAGGATCATCCATGACAGTAAAAACGGAATCATCGGCAGCTGCATTCAGTGACGCa < 1:79557/71‑1 (MQ=255) ttttttAGCGCTGGCAGGATCATCCATGACAGTAAAAACGGAATCATCGGCAGTTGCATTCAGTGa < 1:221284/66‑1 (MQ=255) ttttttAGCGCTGGCAGGATCATCCATGACAGTAAAAACGGAATCATCGGCAGTTGCATTCAGTGa < 1:2082705/66‑1 (MQ=255) ttttttAGCGCTGGCAGGATCATCCATGACAGTAAAAACGGAATCATCGGCAGCTGCATTCAGTGACGCaa < 1:1075558/71‑1 (MQ=255) ttttAGCGCTGGCAGGATCATCCATGACAGTAAAAACGGAATCATCGGCAGTTGCATTCAGTGACGCaaa < 1:670917/70‑1 (MQ=255) ttttAGCGCTGGCAGGATCATCCATGACAGTAAAAACGGAATCATCGGCAGTTGCATTCAGTGACGCaaa < 1:464793/70‑1 (MQ=255) ttttAGCGCTGGCAGGATCATCCATGACAGTAAAAACGGAATCATCGGCAGTTGCATTCAGTGACGCaaa < 1:1364445/70‑1 (MQ=255) ttttAGCGCTGGCAGGATCATCCATGACAGTAAAAACGGAATCATCGGCAGTTGCATTCAGTGACGCaaa < 1:2536069/70‑1 (MQ=255) atcatcCATGACAGTAAAAACGGAATCATCGGCAGCTGCATTCAGTGACGCaaa < 1:897746/54‑1 (MQ=255) tcatcCATGACAGTAAAAACGGAATCATCGGCAGTTGCATTCAGTGACGCAAACATGCCCCCCGCCAAc > 1:1720194/1‑69 (MQ=255) tcatcCATGACAGTAAAAACGGAATCATCGGCAGTTGCATTCAGTGACGCAAACATGCCCCCCGCCAACAt < 1:79580/71‑1 (MQ=255) tcatcCATGACAGTAAAAACGGAATCATCGGCAGCTGCATTCAGTGACGCAAACATGCCCCCCGCCAACa > 1:2761906/1‑70 (MQ=255) tcCATGACAGTAAAAACGGAATCATCGGCAGTTGCATTCAGTGACGCa < 1:1166019/48‑1 (MQ=255) tcCATGACAGTAAAAACGGAATCATCGGCAGCTGCATTCAg > 1:2529821/1‑41 (MQ=255) tcCATGACAGTAAAAACGGAATCATCGGCAGCTGCATTCAg > 1:1174506/1‑41 (MQ=255) tcCATGACAGTAAAAACGGAATCATCGGCAGCTGCATTCAGTGACGCaaa < 1:1998794/50‑1 (MQ=255) cATGACAGTAAAAACGGAATCATCGGCAGTTGCATTCAGTGACGCAAACa > 1:1429933/1‑50 (MQ=255) cATGACAGTAAAAACGGAATCATCGGCAGCTGCATTCAGTGACGCAAACATGCCCCCCGCCAACATAACGa > 1:2367601/1‑71 (MQ=255) cATGACAGTAAAAACGGAATCATCGGCAGCTGCATTCAGTGACGCAAACATGCCCCCCGCCAACATAACGa > 1:107498/1‑71 (MQ=255) tGACAGTAAAAACGGAATCATCGGCAGCTGCATTc < 1:2688267/35‑1 (MQ=255) aCAGTAAAAACGGAATCATCGGCAGTTGCATTCAGTGACGCAAACATGCCCCCCGCCAACATAACGATgg > 1:1968497/1‑70 (MQ=255) aCAGTAAAAACGGAATCATCGGCAGCTGCATTCAg > 1:703769/1‑35 (MQ=255) gTAAAAACGGAATCATCGGCAGCTGCATTCAGTGACGCaaa < 1:2707502/41‑1 (MQ=255) gTAAAAACGGAATCATCGGCAGCTGCATTCAGTGACGCaaa < 1:1597697/41‑1 (MQ=255) aaaaCGGAATCATCGGCAGCTGCATTCAGTGACGCAAACATGCCCCCCGTCAACATAACGATGGCAGGAAc > 1:2699713/1‑71 (MQ=255) atcatcGGCAGCTGCATTCAGTGACGCAAACATGCCCCCCGCCAACATAAc < 1:2509126/51‑1 (MQ=255) atcGGCAGTTGCATTCAGTGACGCAAACATGCCCCCCGCCAACATaa > 1:1034986/1‑47 (MQ=255) atcGGCAGTTGCATTCAGTGACGCAAACATGCCCCCCGCCAACATAACGATGGCAGGAACTGTCTTCaaa > 1:2077918/1‑70 (MQ=255) atcGGCAGTTGCATTCAGTGACGCAAACATGCCCCCCGCCAACATAACGATGGCAGGAACTGTCTTCaaa < 1:573350/70‑1 (MQ=255) gCAGCTGCATTCAGTGACGCAAACATGCCCCCCGCCAACAt < 1:2573319/41‑1 (MQ=255) | TACCTTCGAAAGGTTTTTTAGCGCTGGCAGGATCATCCATGACAGTAAAAACGGAATCATCGGCAGCTGCATTCAGTGACGCAAACATGCCCCCCGCCAACATAACGATGGCAGGAACTGTCTTCAAA > W3110S.gb/1807662‑1807789 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |