| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,810,661 | A→G | 73.0% | F96S (TTT→TCT) | yniB ← | predicted inner membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,810,661 | 0 | A | G | 73.0% | 60.8 / 23.3 | 37 | F96S (TTT→TCT) | yniB | predicted inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base A (5/5); new base G (17/10); total (22/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.08e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGACAAAAATCAGGATGAAAATCACCCAGAACATAACGTTCACGCCACCATTAAAATTGGGCGTCGGGGAGT > W3110S.gb/1810597‑1810727 | ttCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGACAGAAAt > 1:2217743/1‑69 (MQ=255)ttCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGACAAAAAt > 1:481069/1‑69 (MQ=255)ttCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGACAAAAAt > 1:1303221/1‑69 (MQ=255) cccGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGACAAAAATCAg < 1:137259/70‑1 (MQ=255) cAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGACAGAAATCAGGATg < 1:139145/70‑1 (MQ=255) agaagCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGACAAAAATCAGGATGaaa < 1:1582529/71‑1 (MQ=255) aagCGCGCCTGGCGACTCATCCGGGCGCCGGAATCCTGCAACGCCAGTCCGACAGAAATCAGGATGAAAAt > 1:1010991/1‑71 (MQ=255) aagCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGACAAAAATCAGGATGAAAAt > 1:143532/1‑71 (MQ=255) ccTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGACAGAAATCAGGATGAAAATCACCCAg < 1:2316021/71‑1 (MQ=255) ggCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGACAGAAATCAGGATGAAAATCACCCAGaa > 1:2091787/1‑70 (MQ=255) aTCCGGGCACCGGAATCCTGCAACGCCAGTCCGACAGAAATCAGGATGAAAATCACCCAGAACATAACGtt < 1:1589506/71‑1 (MQ=255) aTCCGGGCACCGGAATCCTGCAACGCCAGTCCGACAGAAATCAGGATGAAAATCACCCAGAACATAACGtt < 1:2028409/71‑1 (MQ=255) tCCGGGCACCGGAATCCTGCAACGCCAGTCCGACAGAAATCAGGATGAAAATCACCCAGAACATAACGtt < 1:377967/70‑1 (MQ=255) cGGGCACCGGAATCCTGCAACGCCAGTCCGACAGAAATCAGGATGaaa > 1:1923131/1‑48 (MQ=255) cGGGCACCGGAATCCTGCAACGCCAGTCCGACAGAAATCAGGATGaaa > 1:2597013/1‑48 (MQ=255) cGGGCACCGGAATCCTGCAACGCCAGTCCGACAGAAATCAGGATGaaa > 1:1062002/1‑48 (MQ=255) cGGGCACCGGAATCCTGCAACGCCAGTCCGACAGAAATCAGGATGAAAATCACCCAGAACATAACGTTCa < 1:2403415/70‑1 (MQ=255) cGGGCACCGGAATCCTGCAACGCCAGTCCGACAGAAATCAGGATGAAAATCACCCAGAACATAACGTTCa > 1:1884009/1‑70 (MQ=255) cGGGCACCGGAATCCTGCAACGCCAGTCCGACAGAAATCAGGATGAAAATCACCCAGAACATAACGTTCa > 1:1701657/1‑70 (MQ=255) cGGGCACCGGAATCCTGCAACGCCAGTCCGACAGAAATCAGGATGAAAATCACCCAGAACATAACGTTCAc > 1:1853138/1‑71 (MQ=255) cGGGCACCGGAATCCTGCAACGCCAGTCCGACAAAAATCAGGATGAAAATCACCCAGAACATAACGTTCAc < 1:2294957/71‑1 (MQ=255) aCCGGAATCCTGCAACGCCAGTCCGACAAAAATCAGGATGAAAATCACCCAGAACATAACGTTCACGccac < 1:2262038/71‑1 (MQ=255) gAATCCTGCAACGCCAGTCCGACAGAAATCAGGATGAAAATCACCCAGAACATAACGTTCACGCCACCAt > 1:2413427/1‑70 (MQ=255) aaTCCTGCAACGCCAGTCCGACAGAAATCAGGATGaa < 1:1375189/37‑1 (MQ=255) cTGCAACGCCAGTCCGACAGAAATCAGGATGAAAATCACCCAGAACATa < 1:2082163/49‑1 (MQ=255) cTGCAACGCCAGTCCGACAGAAATCAGGATGAAAATCACCCAGAACATAACGTTCa > 1:1515307/1‑56 (MQ=255) cTGCAACGCCAGTCCGACAGAAATCAGGATGAAAATCACCCAGAACATAACGTTCACGccac > 1:2040394/1‑62 (MQ=255) cTGCAACGCCAGTCCGACAGAAATCAGGATGAAAATCACCCAGAACATAACGTTCACGCCACCATTaaaa > 1:2037924/1‑70 (MQ=255) cTGCAACGCCAGTCCGACAGAAATCAGGATGAAAATCACCCAGAACATAACGTTCACGCCACCATTaaaa > 1:2717081/1‑70 (MQ=255) cTGCAACGCCAGTCCGACAGAAATCAGGATGAAAATCACCCAGAACATAACGTTCACGCCACCATTAAAAt > 1:6943/1‑71 (MQ=255) cTGCAACGCCAGTCCGACAGAAATCAGGATGAAAATCACCCAGAACATAACGTTCACGCCACCATTAAAAt > 1:233852/1‑71 (MQ=255) cTGCAACGCCAGTCCGACAAAAATCAGGATGAAAATCACCCAGAACATAACGTTCACGCCACCATTAAAAt > 1:1931763/1‑71 (MQ=255) cTGCAACGCCAGTCCGACAAAAATCAGGATGAAAATCACCCACAACATAACGTTCACGCCACCATTAAAAt > 1:114330/1‑71 (MQ=255) ccAGTCCGACAAAAATCAGGATGAAAATCACCCAGAACATAACGTTCACGCCACCATTAAAAtt < 1:1367543/64‑1 (MQ=255) ccGACAGAAATCAGGTTGAAAATCACCCAGAACATAACGTTCACGCCACCATTAAAATTGGGCGTCggg > 1:1669447/1‑69 (MQ=255) cGACAGAAATCAGGATGAAAATCACCCAGAACATAACGTTCACGCCACCATTAAAATTGGGCGTCGGGGa < 1:2220439/70‑1 (MQ=255) gACAGAAATCAGGATGAAAATCACCCAGAACATAACGTTCACGCCACCATTAAAATTGGGCGTCGGGGAGt < 1:1174620/71‑1 (MQ=255) | TTCCCGCAAGAAGCGCGCCTGGCGACTCATCCGGGCACCGGAATCCTGCAACGCCAGTCCGACAAAAATCAGGATGAAAATCACCCAGAACATAACGTTCACGCCACCATTAAAATTGGGCGTCGGGGAGT > W3110S.gb/1810597‑1810727 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |