| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,824,257 | T→C | 13.3% | F29S (TTT→TCT) | nadE → | NAD synthetase, NH3/glutamine‑dependent |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,824,257 | 0 | T | C | 13.3% | 70.4 / 5.1 | 30 | F29S (TTT→TCT) | nadE | NAD synthetase, NH3/glutamine‑dependent |
| Reads supporting (aligned to +/- strand): ref base T (13/13); new base C (2/2); total (15/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.90e-01 | |||||||||||
TAATAAAGGCGCTGGGCGCAAAACCGCAGATTAATGCTGAAGAGGAAATTCGTCGTAGTGTCGATTTTCTGAAAAGCTACCTGCAAACTTATCCGTTCATTAAATCACTGGTGCTCGGGATCAGCGGCGGTCAGGA > W3110S.gb/1824191‑1824326 | taataaAGGCGCTGGGCGCAAAACCGCAGATTAATGCTGAAGAGGAAATTCGTCGTAGTGTCGATTTTCt > 1:1024939/1‑70 (MQ=255) ggCGCTGGGCGCAAAACCGCAGATTAATGCTGAAGAGGAAATTCGTCGTAGTGTCGATTTTCTGAAAAGCt < 1:348268/71‑1 (MQ=255) ggCGCTGGGCGCAAAACCGCAGATTAATGCTGAAGAGGAAATTCGTCGTAGTGTCGATTTTCTGAAAAGCt < 1:1532657/71‑1 (MQ=255) gcgcTGGGCGCAAAACCGCAGATTAATGCTGAAGAGGAAATTCGTCGTAGTGTCGATTTTCTGAAAAGCt < 1:1222441/70‑1 (MQ=255) gcgcAAAACCGCAGATTAATGCTGAAGAGGAAATTCGTCGTAGTGTCGATTTTCTGAAAAGCTACCTGCaa < 1:285647/71‑1 (MQ=255) aTTAATGCTGAAGAGGAAATTCGTCGTAGTGTCGATTTTCTGAAAAGCt < 1:2176043/49‑1 (MQ=255) aaTGCTGAAGAGGAAATTCGTCGTAGTGTCGATTTTCTGAAAAGCt < 1:2526648/46‑1 (MQ=255) aaTGCTGAAGAGGAAATTCGTCGTAGTGTCGATTCTCTGAAAAGCTACCTGCAAACTTATCCGTTCATTa < 1:700487/70‑1 (MQ=255) cTGAAGAGGAAATTCGTCGTAGTGTCGATTTTCTGAAAAGCTAc > 1:2278963/1‑44 (MQ=255) cTGAAGAGGAAATTCGTCGTAGTGTCGATTTTCTGAAAAGCTAc > 1:2282194/1‑44 (MQ=255) aaGAGGAAATTCGTCGTAGTGTCGATTTTCTGAAAAGCTACCTGCAAACTTATCCGTTCATTAAATCAc < 1:1638445/69‑1 (MQ=255) aTTCGTCGTAGTGTCGATTCTCTGAAAAGCTACCTGCAAACTTATCCGTTCATTAAATCACTGGTGCTCgg > 1:2312667/1‑71 (MQ=255) ttCGTCGTAGTGTCGATTTTCTGAAAAGCTACCTGCAAACTTATCCGtt < 1:2765203/49‑1 (MQ=255) ttCGTCGTAGTGTCGATTTTCTGAAAAGCTACCTGCAAACTTATCCGTTCATTAAATCACTGGTGCTCgg > 1:1520647/1‑70 (MQ=255) ttCGTCGTAGTGTCGATTCTCTGAAAAGCTACCTGCAAACTTATCCGTTCATTAAATCACTGGTGCTCggg < 1:8698/71‑1 (MQ=255) gtAGTGTCGATTTTCTGAAAAGCTACCTGCAAACTTATCCGTTCATTAAATCACTGGTGCTCGGGATCAgc < 1:1925938/71‑1 (MQ=255) gtgtCGATTTTCTGAAAAGCTACCTGCAAACTTATCCGTTCATTaa < 1:1785783/46‑1 (MQ=255) gtgtCGATTTTCTGAAAAGCTACCTGCAAACTTATCCGTTCATTAAATCACTGGTGCTCggg < 1:2268302/62‑1 (MQ=255) gtgtCGATTTTCTGAAAAGCTACCTGCAAACTTATCCGTTCATTAAATCACTGGTGCTCggg < 1:2271363/62‑1 (MQ=255) tgtCGATTTTCTGAAAAGCTACCTGCAAACTTATCCGTTCATTAAATCACTGGTGCTc < 1:2313951/58‑1 (MQ=255) tCGATTTTCTGAAAAGCTACCTGCAAACTTATCCGTTCAt > 1:1450425/1‑40 (MQ=255) tCGATTTTCTGAAAAGCTACCTGCAAACTTATCCGTTCAt > 1:1218468/1‑40 (MQ=255) tCGATTTTCTGAAAAGCTACCTGCAAACTTATCCGTTCATTAAATCAGTgg > 1:2011973/1‑51 (MQ=255) tCGATTTTCTGAAAAGCTACCTGCAAACTTATCCGTTCATTAAATCACTGGTGCTCGGGATCAGCGGCGGt > 1:2718509/1‑71 (MQ=255) tCGATTCTCTGAAAAGCTACCTGCAATCTTATCCGTTCATTAAATCAc > 1:1839442/1‑48 (MQ=255) gATTTTCTGAAAAGCTACCTGCAAACTTATCCGTTCATTAAATCACTGGTGCTCGGGATCAGcggcgg > 1:2620304/1‑68 (MQ=255) gATTTTCTGAAAAGCTACCTGCAAACTTATCCGTTCATTAAATCACTGGTGCTCGGGATCAGCGGCGGt > 1:149334/1‑69 (MQ=255) gATTTTCTGAAAAGCTACCTGCAAACTTATCCGTTCATTAAATCACTGGTGCTCGGGATCAGCGGCGGt > 1:1340230/1‑69 (MQ=255) gATTTTCTGAAAAGCTACCTGCAAACTTATCCGTTCATTAAATCACTGGTGCTCGGGATCAGCGGCGGt > 1:660054/1‑69 (MQ=255) gATTTTCTGAAAAGCTACCTGCAAACTTATCCGTTCATTAAATCACTGGTGCTCGGGATCAGCGGCGGt > 1:1065531/1‑69 (MQ=255) tttCTGAAAAGCTACCTGCAAACTTATCCGTTCATTAAATCACTGGTGCTCGGGATCAGCGGCGGTCATGa > 1:85649/1‑71 (MQ=255) ttCTGAAAAGCTACCTGCAAACTTATCCGTTCATTAAATCACTg > 1:1253014/1‑44 (MQ=255) | TAATAAAGGCGCTGGGCGCAAAACCGCAGATTAATGCTGAAGAGGAAATTCGTCGTAGTGTCGATTTTCTGAAAAGCTACCTGCAAACTTATCCGTTCATTAAATCACTGGTGCTCGGGATCAGCGGCGGTCAGGA > W3110S.gb/1824191‑1824326 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |