| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,832,787 | T→C | 21.6% | M304V (ATG→GTG) | astC ← | succinylornithine transaminase, PLP‑dependent |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,832,787 | 0 | T | C | 21.6% | 70.5 / 17.1 | 37 | M304V (ATG→GTG) | astC | succinylornithine transaminase, PLP‑dependent |
| Reads supporting (aligned to +/- strand): ref base T (14/15); new base C (5/3); total (19/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.93e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CCATAGCGGTGATTAATAGTATTAAGACGCTCAACAAACCAGTCGTGACGCTGTTTAACGCCATTAAGCATCTCTGGTGTGTTGATGAGCTCCAGCACTTTGCCTGCCACCGCCGAGGCCAGCGGATTACCGCCA > W3110S.gb/1832717‑1832851 | ccATAGCGGTGATTAATAGTATTAAGACGCTCAACAAACCAGTCGTGACGCTGTTTAACGCCATTAAGCAt < 1:578080/71‑1 (MQ=255) aTTAATAGTATTAAGACGCTCAACAAACCAGTCGTGACGCTGTTTAACGCCATTAAGCATCTCTGgtgtg < 1:1676169/70‑1 (MQ=255) ttAATAGTATTAAGACGCTCAACAAACCAGTCGTGACGCTGTTTAACGCCATTAAGCATCTCTGGTGTGtt < 1:1321037/71‑1 (MQ=255) tAATAGTATTAAGACGCTCAACAAACCAGTCGTGACGCTGTTTAACGCCATTAAGCATCTCTGGTGTGtt < 1:1828227/70‑1 (MQ=255) tAGTATTAAGACGCTCAACAAACCAGTCGTGACGCTGTTTAACGCCATTAAGCATCTCTGgtgt < 1:969850/64‑1 (MQ=255) tAGTATTAAGACGCTCAACAAACCAGTCGTGACGCTGTTTAACGCCATTAAGCATCTCTGgtgt < 1:640367/64‑1 (MQ=255) tAGTATTAAGACGCTCAACAAACCAGTCGTGACGCTGTTTAACGCCATTAAGCATCTCTGGTGTGTtgatg < 1:2217271/71‑1 (MQ=255) tAGTATTAAGACGCTCAACAAACCAGTCGTGACGCTGTTTAACGCCATTAAGCATCTCTGGTGTGTtgatg < 1:2543044/71‑1 (MQ=255) tATTAAGACGCTCAACAAACCAGTCGTGACGCTGTTTAACGCCATTAAGCACCTCTGGTGTGtt < 1:1776000/64‑1 (MQ=255) tATTAAGACGCTCAACAAACCAGTCGTGACGCTGTTTAACGCCATTAAGCACCTCTGGTGTGtt < 1:1779254/64‑1 (MQ=255) gACGCTCAACAAACCAGTCGTGACGCTGTTTAACGCCATTAAGCACCTCTGGTGTGTTGATGAGCTCCAGc < 1:1516146/71‑1 (MQ=255) caaACCAGTCGTGACGCTGTTTAACGCCATTAAGCATCTCTGGTGTGTtga > 1:1921578/1‑51 (MQ=255) caaACCAGTCGTGACGCTGTTTAACGCCATTAAGCATCTCTGGTGTGTtga > 1:1946787/1‑51 (MQ=255) caaACCAGTCGTGACGCTGTTTAACGCCATTAAGCATCTCTGGTGTGTtga > 1:430783/1‑51 (MQ=255) aaaCCAGTCGTGACGCTGTTTAACGCCATTAAGCATCTCTGGTGTGTTGATGAGCTCCAGCAc > 1:1811983/1‑63 (MQ=255) aaaCCAGTCGTGACGCTGTTTAACGCCATTAAGCACCTCTGGTGTGTTGATGAGCTCCAGCACTTtgcctg > 1:1261106/1‑71 (MQ=255) gTCGTGACGCTGTTTAACGCCATTAAGCATCTCTGGTGTGTTGATGAGc > 1:118764/1‑49 (MQ=255) tCGTGACGCTGTTTAACGCCATTAAGCATCTCTGgtg < 1:1870593/37‑1 (MQ=255) gTGACGCTGTTTAACGCCATTAAGCATCTCTGGTGTGTTGATGAGCTCCAGCACTTTGCCTGCCAccgcc > 1:972262/1‑70 (MQ=255) gTGACGCTGTTTAACGCCATTAAGCACCTCTGGTGTGTTGATGAGCTCCAGCACTTTGCCTGCCAccgcc > 1:1286806/1‑70 (MQ=255) tttAACGCCATTAAGCATCTCTGGTGTGTTGATGAGCTCCAGCACttt < 1:641608/48‑1 (MQ=255) tttAACGCCATTAAGCATCTCTGGTGTGTTGATGAGCTCCAGCACTTTGCCTGCCACCGCCGAGGCCAg < 1:1432458/69‑1 (MQ=255) tttAACGCCATTAAGCATCTCTGGTGTGTTGATGAGCTCCAGCACTTTGCCTGCCACCGCCGAGGCCAGCg < 1:525194/71‑1 (MQ=255) tAACGCCATTAAGCACCTCTGGTGTGTTGATGAGCTCCAGCACTTTGCCTGCCACCGCCGAGGCCAGCGGa > 1:377953/1‑71 (MQ=255) tAACGCCATTAAGCACCTCTGGTGTGTTGATGAGCTCCAGCACTTTGCCTGCCACCGCCGAGGCCAGCGGa > 1:381201/1‑71 (MQ=255) aCGCCATTAAGCACCTCTGGTGTGTTGATGAGCTCCAGCACTTTGCCTGCCACCGCCGAGCCCAGCGGAt > 1:2501418/1‑70 (MQ=255) aTTAAGCATCTCTGGTGTGTTGATGAGCTCCAGCAc < 1:1288359/36‑1 (MQ=255) tAAGCATCTCTGGTGTGTTGATGAGCTCCAGCACTTTGCCTGCCACCGCCGa > 1:41388/1‑52 (MQ=255) aaGCATCTCTGGTGTGTTGATGAGCTCCAGCACTTtgcc > 1:548727/1‑39 (MQ=255) aaGCATCTCTGGTGTGTTGATGAGCTCCAGCACTTtgcc > 1:863192/1‑39 (MQ=255) aaGCATCTCTGGTGTGTTGATGAGCTCCAGCACTTtgcc > 1:2798934/1‑39 (MQ=255) aaGCATCTCTGGTGTGTTGATGAGCTCCAGCACTTtgc > 1:15974/1‑38 (MQ=255) aaGCATCTCTGGTGTGTTGATGAGCTCCAGCACTTTGCCTGCCACCGCCGAGGCCAGCGGATTACCGCCa > 1:579172/1‑70 (MQ=255) aaGCATCTCTGGTGTGTTGATGAGCTCCAGCACTTTGCCTGCCACCGCCGAGGCCAGCGGATTACCGCCa < 1:2050181/70‑1 (MQ=255) aaGCATCTCTGGTGTGTTGATGAGCTCCAGCACTTTGCCTGCCACCGCCGAGGCCAGCGGATTACCGCCa > 1:21680/1‑70 (MQ=255) aaGCATCTCTGGTGTGTTGATGAACTCCAGCACTTtgcc > 1:263560/1‑39 (MQ=255) gCATCTCTGGTGTGTTGATGAGCTCCAGCACTTTGCCTGCCACCGCCGAGGCCAGCGGATTACCGCCa < 1:657198/68‑1 (MQ=255) gCATCTCTGGTGTGTTGATGAGCTCCAGCACTTTGCCTGCCACCGCCGAGGCCAGCGGATTACCGCCa < 1:866504/68‑1 (MQ=255) | CCATAGCGGTGATTAATAGTATTAAGACGCTCAACAAACCAGTCGTGACGCTGTTTAACGCCATTAAGCATCTCTGGTGTGTTGATGAGCTCCAGCACTTTGCCTGCCACCGCCGAGGCCAGCGGATTACCGCCA > W3110S.gb/1832717‑1832851 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |