Predicted mutation
evidence seq id position mutation freq annotation gene description
RA W3110S.gb 1,837,528 G→A 100% V100V (GTG→GTA)  ynjA → conserved hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb1,837,5280GA75.0% 23.0 / 6.4 16V100V (GTG→GTA) ynjAconserved hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (1/3);  new base A (6/6);  total (7/9)
Fisher's exact test for biased strand distribution p-value = 5.85e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.59e-01
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

TTGTGTGGATATCACCAGTATGAAAGTCGCCGAGCGCACCGGCAGCAGCGATAAACTGCTGGCAGTGGCTGACTGGCGGCAAAGCCCGCTCTTTAGCGATGAAGAACGGCTGGCGCTGGAGTA  >  W3110S.gb/1837462‑1837584
                                                                  |                                                        
ttGTGTGGATATCACCAGTATGAAAGTCGCCGAGCGCACCGGCAGCAGCGATAAACTGCTGGCAGTAGCt                                                       >  1:911530/1‑70 (MQ=255)
 tgtgtgGATATCACCAGTATGAAAGTCGCCGAGCGCACCGGCAGCAGCGATAAACTGCTGGCAGTGGCTg                                                      <  1:2485940/70‑1 (MQ=255)
       gATATCACCAGTATGAAAGTCGCCGAGCGCACCGGCAGCAGCGATAAACTGCTGGCAGTAGCTGACTggcg                                               >  1:2518088/1‑71 (MQ=255)
            cACCAGTATGAAAGTCGCCGAGCGCACCGGCAGCAGCGATAAACTGCTGGCAGTAGCTGAc                                                    >  1:1622924/1‑61 (MQ=255)
             aCCAGTATGAAAGTCGCCGAGCGCACCGGCAGCAGCGATAAACTGCTGGCAGTGGCTGACTGGCGGCAAAg                                         >  1:2319969/1‑71 (MQ=255)
                      aaaGTCGCCGAGCGCACCGGCAGCAGCGATAAACTGCTGGCAGTAGCTGa                                                     >  1:2729793/1‑50 (MQ=255)
                      aaaGTCGCCGAGCGCACCGGCAGCAGCGATAAACTGCTGGCAGTAGCTGACTGGCGGCAAAGCCCGCTCtt                                >  1:1333969/1‑71 (MQ=255)
                              cGAGCGCACCGGCAGCAGCGATAAACTGCTGGCAGTAGCt                                                       <  1:2239941/40‑1 (MQ=255)
                                 gcgcACCGGCAGCAGCGATAAACTGCTGGCAGTGGCTGACTGGCGGCAAAGCCCGCTCTTTAGCGATgaag                     <  1:1572299/71‑1 (MQ=255)
                                   gcACCGGCAGCAGCGATAAACTGCTGGCAGTGGCTGACTGGCGGCAAAGCCCGCTCTTTAGCGATgaagaa                   <  1:2413370/71‑1 (MQ=255)
                                      ccGGCAGCAGCGATAAACTGCTGGCAGTAGCTGACTGGCGGCAAAGCCCGCTCTTTAGCGATGAAGAACgg                <  1:207150/71‑1 (MQ=255)
                                                cGATAAACTGCTGGCAGTAGCTGACTGGCGGCAAAGCCCGCTCTTTAGCGATGAAGAACGGCTGGCGCTgg      <  1:2588959/71‑1 (MQ=255)
                                                  aTAAACTGCTGGCAGTAGCTGACTGGCGGCAAAGCCCGCTCTTTa                              <  1:1135693/45‑1 (MQ=255)
                                                  aTAAACTGCTGGCAGTAGCTGACTGGCGGCAAAGCCCGCTCTTTa                              <  1:915756/45‑1 (MQ=255)
                                                   tAAACTGCTGGCAGTAGCTGACTGGCGGCAAAGCCCGCTCTTTAGCGATgaag                     <  1:1312979/53‑1 (MQ=255)
                                                    aaaCTGCTGGCAGTAGCTGACTGGCGGCAAAGCCCGCTCTTTAGCGATGAAGAACGGCTGGCGCTGGAGTa  >  1:2297258/1‑71 (MQ=255)
                                                                  |                                                        
TTGTGTGGATATCACCAGTATGAAAGTCGCCGAGCGCACCGGCAGCAGCGATAAACTGCTGGCAGTGGCTGACTGGCGGCAAAGCCCGCTCTTTAGCGATGAAGAACGGCTGGCGCTGGAGTA  >  W3110S.gb/1837462‑1837584

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑