| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,865,587 | T→C | 100% | L8L (CTT→CTC) | yeaD → | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,865,587 | 0 | T | C | 79.2% | 48.9 / 8.0 | 24 | L8L (CTT→CTC) | yeaD | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base T (4/1); new base C (9/10); total (13/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.27e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.16e-01 | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
GCTCTTTTTTTTACCTGATAAAATGAAGTTAAAGGACTGCGTCATGATTAAGAAAATTTTTGCCCTTCCGGTCATCGAACAAATCTCCCCTGTCCTCTCCCGTCGTAAACTGGATGAACT > W3110S.gb/1865521‑1865640 | gCTCTTTTTTTTACCTGATAAAATGAAGTTAAAGGACTGCGTCATGATTAAGAAAATTTTTGCCCTTCCgg > 1:850602/1‑71 (MQ=255) ttttttACCTGATAAAATGAAGTTAAAGGACTGCGTCATGATTAAGAAAATTTTTGCCCTCCCGGTCAt > 1:1569399/1‑69 (MQ=255) tACCTGATAAAATGAAGTTAAAGGACTGCGTCATGATTAAGAAAATTTTTGCCCTCCCGGTCATCGAACa < 1:2452655/70‑1 (MQ=255) tACCTGATAAAATGAAGTTAAAGGACTGCGTCATGATTAAGAAAATTTTTGCCCTCCCGGTCATCGAACa < 1:2666224/70‑1 (MQ=255) tACCTGATAAAATGAAGTTAAAGGACTGCGTCATGATTAAGAAAATTTTTGCCCTCCCGGTCATCGAACa < 1:579259/70‑1 (MQ=255) tACCTGATAAAATGAAGTTAAAGGACTGCGTCATGATTAAGAAAATTTTTGCCCTCCCGGTCATCGAACa < 1:1806125/70‑1 (MQ=255) aTAAAATGAAGTTAAAGGACTGCGTCATGATTAAGAAAATTTTTGCCCTCCCGGTCATCGAACAAAtctc < 1:1794224/70‑1 (MQ=255) aaaaTGAAGTTAAAGGACTGCGTCATGATTAAGAAAATTTTTGCCCTCCCGGTCATCGAACAAATCTcccc > 1:1261169/1‑71 (MQ=255) tGAAGTTAAAGGACTGCGTCATGATTAAGAATATTTTTGCCCTTCGGGTCATCGAACAAATCTCCCCTGTc > 1:2597316/1‑71 (MQ=255) tGAAGTTAAAGGACTGCGTCATGATTAAGAAAATTTTTGCCCTCCCGGTCATCGAACAAATCTCCCCTGTc < 1:2141324/71‑1 (MQ=255) gTTAAAGGACTGCGTCATGATTAAGAAAATTTTTGCCCTCCCGGTCATCGAACAAATCTCCCCTGTCctct < 1:2534774/71‑1 (MQ=255) ttAAAGGACTGCGTCATGATTAAGAAAATTTTTGCCCTCCCGGt < 1:1901361/44‑1 (MQ=255) aaaGGACTGCGTCATGATTAAGAAAATTTTTGCCCTCCCGGTCATCGAACAAATCTCCCCTGTCCTCTccc > 1:940856/1‑71 (MQ=255) aGGACTGCGTCATGATTAAGAAAATTTTTGCCCTCCCGGTCATCGAACaaa > 1:1661585/1‑51 (MQ=255) aCTGCGTCATGATTAAGAAAATTTTTGCCCTCCCGGTCATCGAACAAATCTCCCCTGTCCTCTCCcgtcgt < 1:1736848/71‑1 (MQ=255) cTGCGTCATGATTAAGAAAATTTTTGCCCTTCCGGTCATCGAACAAATCTCCCCTGTCCTCTCCCGTCGTa < 1:261429/71‑1 (MQ=255) tCATGATTAAGAAAATTTTTGCCCTCCCGGTCATCGAACa < 1:2335430/40‑1 (MQ=255) aTGATTAAGAAAATTTTTGCCCTCCCGGTCATCGAACaaa > 1:1052188/1‑40 (MQ=255) aTGATTAAGAAAATTTTTGCCCTCCCGGTCATCGAACaaa > 1:2127109/1‑40 (MQ=255) aTGATTAAGAAAATTTTTGCCCTCCCGGTCATCGAACAAATCTCCCCTGTCCTCTCCcgtcg > 1:1407568/1‑62 (MQ=255) gATTAAGAAAATTTTTGCCCTCCCGGTCATCGAACAAATCTCCCCTGTCCTCTCCCGTCGTaa > 1:574244/1‑63 (MQ=255) tAAGAAAATTTTTGCCCTCCCGGTCATCGAACAAATCTCCCCTGTCCTCTCCCGTCGTAAACTGGATGaa > 1:945163/1‑70 (MQ=255) aaGAAAATTTTTGCCCTTCCGGTCATCGAACAAATCTCCCCTGTCCTCTCCCGTCGTAAAc > 1:1408419/1‑61 (MQ=255) aaGAAAATTTTTGCCCTTCCGGTCATCGAACAAATCTCCCCTGTCCTCTCCCGTCGTAAACTGGATGAACt > 1:2466543/1‑71 (MQ=255) | GCTCTTTTTTTTACCTGATAAAATGAAGTTAAAGGACTGCGTCATGATTAAGAAAATTTTTGCCCTTCCGGTCATCGAACAAATCTCCCCTGTCCTCTCCCGTCGTAAACTGGATGAACT > W3110S.gb/1865521‑1865640 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |