| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 286,496 | T→C | 35.3% | F162L (TTT→CTT) | yagH → | predicted xylosidase/arabinosidase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 286,496 | 0 | T | C | 35.3% | 9.7 / 13.3 | 17 | F162L (TTT→CTT) | yagH | predicted xylosidase/arabinosidase |
| Reads supporting (aligned to +/- strand): ref base T (5/6); new base C (2/4); total (7/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.61e-01 | |||||||||||
TATCTACCGCCCGTGGGGGCCGCGCCACCACAGCAACCCGCACAACACCATCGTGTTACAGGCGTTTGACCCGCAGACCGGCACGCTCTCGCCCGAGCGCAAAACGCTGTTTACCGGCACGCCGCTCTG > W3110S.gb/286432‑286560 | tatCTACCGCCCGTGGGGGCCGCGCCACCACAGCAACCCGCACAACACCATCGTGTTACAGGCGTTTg < 1:1542479/68‑1 (MQ=255)tatCTACCGCCCGTGGGGGCCGCGCCACCACAGCAACCCGCACAACACCATCGTGTTACAGGCGTTTg < 1:561117/68‑1 (MQ=255) ccGCCCGTGGGGGCCGCGCCACCACAGCAACCCGCACAACACCATCGTGTTACAGGCGTTTGACCCGCAg < 1:1123009/70‑1 (MQ=255) ccGCCCGTGGGGGCCGCGCCACCACAGCAACCCGCACAACACCATCGTGTTACAGGCGTTTGACCCGCAg < 1:1240147/70‑1 (MQ=255) accacAGCAACCCGCACAACACCATCGTGTTACAGGCGCTTGACCCGCAg < 1:2114793/50‑1 (MQ=255) accacAGCAACCCGCACAACACCATCGTGTTACAGGCGCTTGACCCGCAGACCGGCACGCTCTCGCCCGAg < 1:2044330/71‑1 (MQ=255) accacAGCAACCCGCACAACACCATCGTGTTACAGGCGCTTGACCCGCAGACCGGCACGCTCTCGCCCGAg < 1:2047653/71‑1 (MQ=255) cGCACAACACCATCGTGTTACAGGCGTTTGACCCGCAGACCGGCACGCTCTCGCCCGAGCGCAAAACGCTg < 1:1444006/71‑1 (MQ=255) cGCACAACACCATCGTGTTACAGGCGTTTGACCCGCAGACCGGCACGCTCTCGCCCGAGCGCAAAACGCTg > 1:2571399/1‑71 (MQ=255) cGCACAACACCATCGTGTTACAGGCGTTTGACCCGCAGACCGGCACGCTCTCGCCCGAGCGCAAAACGCTg > 1:1969013/1‑71 (MQ=255) cacaACACCATCGTGTTACAGGCGCTTGACCCGCAGACCGGCACGCTCTCGCCCGAGCGCaaaa > 1:1642049/1‑64 (MQ=255) acacCATCGTGTTACAGGCGCTTGACCCGCAGACCGGCACGCTCTCGCCCGAGCGCAAAACGCTGTTTAc > 1:90609/1‑70 (MQ=255) acCATCGTGTTACAGGCGCTTGACCCGCAGACCGGCACGCTCTCGCCCGAGCGCAAAACGCTGTTTACCgg < 1:1736519/71‑1 (MQ=255) gtgtTACAGGCGTTTGACCCGCAGACCGGCACGCTCTCGCCCGAGCGCAAAACGCTGTTTACCGGCAcgc < 1:328442/70‑1 (MQ=255) gtgtTACAGGCGTTTGACCCGCAGACCGGCACGCTCTCGCCCGAGCGCAAAACGCTGTTTACCGGCAcgc > 1:1563833/1‑70 (MQ=255) gtgtTACAGGCGTTTGACCCGCAGACCGGCACGCTCTCGCCCGAGCGCAAAACGCTGTTTACCGGCAcgc > 1:818752/1‑70 (MQ=255) cAGGCGTTTGACCCGCAGACCGGCACGCTCTCGCCCGAGCGCAAAACGCTGTTTACCGGCACGCCGCTCTg > 1:1136723/1‑71 (MQ=255) | TATCTACCGCCCGTGGGGGCCGCGCCACCACAGCAACCCGCACAACACCATCGTGTTACAGGCGTTTGACCCGCAGACCGGCACGCTCTCGCCCGAGCGCAAAACGCTGTTTACCGGCACGCCGCTCTG > W3110S.gb/286432‑286560 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |