| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,873,346 | C→T | 32.2% | L416L (CTC→CTT) | yeaI → | predicted diguanylate cyclase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,873,346 | 0 | C | T | 32.2% | 37.5 / 22.7 | 31 | L416L (CTC→CTT) | yeaI | predicted diguanylate cyclase |
| Reads supporting (aligned to +/- strand): ref base C (8/13); new base T (4/6); total (12/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.70e-01 | |||||||||||
AGCATCCGCCCCGATGATATTTTAGCGCGACTGGAGGGTGAGGTGTTTGGCTTGCTATTTACCGAACTCAATAGTGCCCAGGCAAAAATCATTGCGGAACGTATGCGTAAAAATGTCGAACTCCTGACCGGCTT > W3110S.gb/1873278‑1873411 | aGCATCCGCCCCGATGATATTTTAGCGCGACTGGAGGGTGAGGTGTTTGGCTTGCTATTTACCGAACTTaa < 1:20590/71‑1 (MQ=255)aGCATCCGCCCCGATGATATTTTAGCGCGACTGGAGGGTGAGGTGTTTGGCTTGCTATTTACCGAACTCaa < 1:1397283/71‑1 (MQ=255)aGCATCCGCCCCGATGATATTTTAGCGCGACTGGAGGGTGAGGTGTTTGGCTTGCTATTTACCGAACTCaa < 1:1571452/71‑1 (MQ=255) cGCCCCGATGATATTTTAGCGCGACTGGAGGGTGAGGTGTTTGGCTTGCTATTTACCGAACTCAATAGTGc > 1:965411/1‑71 (MQ=255) cGCCCCGATGATATTTTAGCGCGACTGGAGGGTGAGGTGTTTGGCTTGCTATTTACCGAACTCAATAGTGc > 1:447299/1‑71 (MQ=255) cGCCCCGATGATATTTTAGCGCGACTGGAGGGTGAGGTGTTTGGCTTGCTATTTACCGAACTCAATAGTGc > 1:389247/1‑71 (MQ=255) gCCCCGATGATATTTTAGCGCGACTGGAGGGTGAGGTGTTTGGCTTGCTATTTACCGAACTCAATAGTGcc < 1:1512982/71‑1 (MQ=255) ccGAAGATATTTTAGCGCGACTGGAGGGTGAGGTGTTTGGCTTGCTATTTACCGAACTTAATAGTGCCCAg > 1:729117/1‑71 (MQ=255) gatATTTTAGCGCGACTGGAGGGTGTGGTGTTTGGCTTGCTATTTACCGAACTTAATAGTg < 1:2401898/61‑1 (MQ=255) tatTTTAGCGCGACTGGAGGGTGAGGTGTTTGGCTTGCTATTTACCGAACTCAATAGTGCCCAGGCaaaaa < 1:1782708/71‑1 (MQ=255) atTTTAGCGCGACTGGAGGGTGAGGTGTTTGGCTTGCTATTTACCGAACTCAATAGTGCCCa > 1:1982235/1‑62 (MQ=255) atTTTAGCGCGACTGGAGGGTGAGGTGTTTGGCTTGCTATTTACCGAACTCAATAGTGCCCa > 1:1562264/1‑62 (MQ=255) ttttAGCGCGACTGGAGGGTGAGGTGTTTGGCTTGCTATTTACCGAACTTAATAGTGCCCAGGCAAAAAt > 1:1066471/1‑70 (MQ=255) cTGGAGGGTGAGGTGTTTGGCTTGCTATTTACCGAACTCAATAGTGCCCAGGCAAAAATCATTGCGGAACg < 1:555524/71‑1 (MQ=255) tGAGGTGTTTGGCTTGCTATTTACCGAACTTAATAGTGCCCAGGCAAAAATCATTGCGGAACGTa > 1:2794799/1‑65 (MQ=255) aGGTGTTTGGCTTGCTATTTACCGAACTCAATAGTGCCCAGGCAAAAATCATTGCGGAACGTATGCGTaaa < 1:2713714/71‑1 (MQ=255) gtgtTTGGCTTGCTATTTACCGAACTTAATAGTGCCCAGGCAAAAATc < 1:1996680/48‑1 (MQ=255) gtgtTTGGCTTGCTATTTACCGAACTCAATAGTGCCCAGGCAAAAAt < 1:1893308/47‑1 (MQ=255) tttGGCTTGCTATTTACCGAACTCAATAGTGCCCAGGCAAAAATCATTGCGGAACGTa > 1:2786313/1‑58 (MQ=255) ttGGCTTGCTATTTACCGAACTCAATAGTGCCCAGGCAAAAATCATTGCGGAACGTa > 1:1947028/1‑57 (MQ=255) tGGCTTGCTATTTACCGAACTTAATAGTGCCCAGGCAAAAATCATTGCGGAACGTATGCGTAAAAATGTc > 1:1144347/1‑70 (MQ=255) ggCTTGCTATTTACCGAACTTAATAGTGCCCAGGCAAAAATCATTGAGGAACGTATGCGTAAAAATGTCg < 1:77419/70‑1 (MQ=255) ggCTTGCTATTTACCGAACTCAATAGTGCCCAGGCAAAAATCATTGCGGAACGTATGCGTAAAAATGTCg < 1:2001850/70‑1 (MQ=255) ttGCTATTTACCGAACTCAATAGTGCCCAGGCAAAAATCAt < 1:718309/41‑1 (MQ=255) gCTATTTACCGAACTCAATAGTGCCCAGGCAAAAATCATTGCGGAACGTATGCGTAAAAATGTCGAACt < 1:1453606/69‑1 (MQ=255) aTTTACCGAACTCAATAGTGCCCAGGCAAAAATCATTGCGGAACGTATGCGTAAAAAt < 1:2111054/58‑1 (MQ=255) tttACCGAACTCAATAGTGCCCAGGCAAAAATCATTGCGGAACg < 1:155333/44‑1 (MQ=255) tACCGAACTCAATAGTGCCCAGGCAAAAATCATTGCGGAACGTATGCGTAAAAATGTCGaa > 1:1728149/1‑61 (MQ=255) gAACTTAATAGTGCCCAGGCAAAAATCATTGCGGAACGTATGCGTAAAAATGTCGAACTCCTGACCGGCtt < 1:1563528/71‑1 (MQ=255) gAACTTAATAGTGCCCAGGCAAAAATCATTGCGGAACGTATGCGTAAAAATGTCGAACTCCTGACCGGCtt < 1:1560426/71‑1 (MQ=255) tCAATAGTGCCCAGGCAAAAATCATTGCGGAACGTATGCGTAAAAATGTCGAACTCCTGACCGGCtt < 1:1797920/67‑1 (MQ=255) | AGCATCCGCCCCGATGATATTTTAGCGCGACTGGAGGGTGAGGTGTTTGGCTTGCTATTTACCGAACTCAATAGTGCCCAGGCAAAAATCATTGCGGAACGTATGCGTAAAAATGTCGAACTCCTGACCGGCTT > W3110S.gb/1873278‑1873411 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |