| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,990,800 | A→G | 100% | intergenic (+118/‑53) | yecR → / → ftn | hypothetical protein/cytoplasmic ferritin iron storage protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,990,800 | 0 | A | G | 78.3% | 43.1 / 8.2 | 23 | intergenic (+118/‑53) | yecR/ftn | hypothetical protein/cytoplasmic ferritin iron storage protein |
| Reads supporting (aligned to +/- strand): ref base A (1/4); new base G (5/13); total (6/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.47e-01 | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
ATGCGTTTTATTCCCATTCGTATTTTTAATAATTGAAGTTTATATTTTACCTTTTGCAAATAATAAAATAACAAATTATAGTGACGCCACAGCAACAAATATAACCTTTGTGGAGCACTATCATGCTGAAACCAGAAA > W3110S.gb/1990731‑1990868 | aTGCGTTTTATTCCCATTCGTATTTTTAATAATTGAAGTTTATATTTTACCTTTTGCAAATaataaaataa > 1:2458304/1‑71 (MQ=255)aTGCGTTTTATTCCCATTCGTATTTTTAATAATTGAAGTTTATATTTTACCTTTTGCAAATaataaaata < 1:1859008/70‑1 (MQ=255) tGCGTTTTATTCCCATTCGTATTTTTAATAATTGAAGTTTATATTTTACCTTTTGCAAATaataaaataa > 1:2050503/1‑70 (MQ=255) gCGTTTTATTCCCATTCGTATTTTTAATAATTGAAGTTTATATTTTACCTTTTGCAAATAATAAAATGAc > 1:97535/1‑70 (MQ=255) cGTTTTATTCCCATTCGTATTTTTAATAATTGAAGTTTATATTTTACCTTTTGCAAATAATAAAATGACaa < 1:814219/71‑1 (MQ=255) cGTTTTATTCCCATTCGTATTTTTAATAATTGAAGTTTATATTTTACCTTTTGCAAATAATAAAATGACaa < 1:810868/71‑1 (MQ=255) cGTTTTATTCCCATTCGTATTTTTAATAATTGAAGTTTATATTTTACCTTTTGCAAATAATAAAATGACa > 1:1398620/1‑70 (MQ=255) ttttATTCCCATTCGTATTTTTAATAATTGAAGTTTATATTTTACCTTTTGCAAATAATAAAATGACAAAt < 1:1480013/71‑1 (MQ=255) ttATTCCCATTCGTATATTTAATAATTGAAGTTTATATTTTACCTTTTGCAAATAATAAAATGACAAATta > 1:541868/1‑71 (MQ=255) tATTCCCATTCGTATTTTTAATAATTGAAGTTTATATTTTACCTTTTGCAAATAATAAAATGACAAATta < 1:565331/70‑1 (MQ=255) tttttAATAATTGAAGTTTATATTTTACCTTTTGCAAATAATAAAATGACa < 1:1304809/51‑1 (MQ=255) tttttAATAATTGAAGTTTATATTTTACCTTTTGCAAATAATAAAATGACAAATTATAGt < 1:513433/60‑1 (MQ=255) ataatTGAAGTTTATATTTTACCTTTTGCAAATAATAAAATGACAAATTATAGTGACGCCACAGCAACaaa < 1:995760/71‑1 (MQ=255) ataatTGAAGTTTATATTTTACCTTTTGCAAATAATAAAATAACAAATTATAGTGACGCCACAGcaaca < 1:2393982/69‑1 (MQ=255) ttATATTTTACCTTTTGCAAATAATAAAATGACAAAt < 1:2144431/37‑1 (MQ=255) tataTTTTACCTTTTGCAAATAATAAAATGACAAATTATAGTGACGCCACAGCAACAAatat < 1:1194013/62‑1 (MQ=255) ttttACCTTTTGCAAATAATAAAATGACAAATTATAGTGACGCCACAGCAACAAATATAACCTTTGTGGAg < 1:1000380/71‑1 (MQ=255) ttttACCTTTTGCAAATAATAAAATGACAAATTATAGTGACGCCACAGCAACAAATATAACCTTTGTGGAg < 1:1991025/71‑1 (MQ=255) ttttACCTTTTGCAAATAATAAAATAACAAATTATAGt < 1:1386562/38‑1 (MQ=255) ttttACCTTTTGCAAATAATAAAATAACAAATTATAGTGACGc < 1:2569162/43‑1 (MQ=255) ttACCTTTTGCAAATAATAAAATAACAAATTATAGTGa > 1:2310538/1‑38 (MQ=255) ttACCTTTTGCAAATAATAAAATAACAAATTATAGTGACGCCACAGCAACAAATATAACCTTTGTGGAGc < 1:1185443/70‑1 (MQ=255) ccTTTTGCAAATAATAAAATGACAAATTATAGTGACGCCACAGCAACAAATATAACCTTTGTGGAGCACTa > 1:561911/1‑71 (MQ=255) cTTTTGCAAATAATAAAATGACAAATTATAGTGACGCCACAGCAACAAATATAACCTTTGTGGAGCACTAt < 1:1591605/71‑1 (MQ=255) tttGCAAATAATAAAATGACAAATTATAGTGACGCCACAGCAACAAATATAACCTTTGTGGAg > 1:1537725/1‑63 (MQ=255) ttGCAAATAATAAAATGACAAATTATAGTGACGCCACAGCAACAAATATAACCTTTGTgg < 1:2688565/60‑1 (MQ=255) taaaatgaCAAATTATAGTGACGCCACAGCAACAAATATAACCTTTGTGGAGCACTATCATGCTGAAAc > 1:1929272/1‑69 (MQ=255) aataaCAAATTATAGTGACGCCACAGCAACAAATATAACCTTTGTGGAGCACTa < 1:1919155/54‑1 (MQ=255) ataaCAAATTATAGTGACGCCACAGCAACAAATATAACCTTTGTGGAGCACTATCATGCTGAAACCAGaaa > 1:654171/1‑71 (MQ=255) | ATGCGTTTTATTCCCATTCGTATTTTTAATAATTGAAGTTTATATTTTACCTTTTGCAAATAATAAAATAACAAATTATAGTGACGCCACAGCAACAAATATAACCTTTGTGGAGCACTATCATGCTGAAACCAGAAA > W3110S.gb/1990731‑1990868 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |