| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,000,742 | C→T | 58.1% | M292I (ATG→ATA) | yedO ← | D‑cysteine desulfhydrase, PLP‑dependent |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,000,742 | 0 | C | T | 58.1% | 11.0 / 36.6 | 31 | M292I (ATG→ATA) | yedO | D‑cysteine desulfhydrase, PLP‑dependent |
| Reads supporting (aligned to +/- strand): ref base C (7/6); new base T (14/4); total (21/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.47e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.74e-01 | |||||||||||
TGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCAAGCAGAATGCCTTCCAGCCGCGCCAGCAATTTCACTGCTTC > W3110S.gb/2000677‑2000810 | tGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCTATCg < 1:2260848/70‑1 (MQ=255) aaTAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCTATCGCtt > 1:1091053/1‑71 (MQ=255) aaTAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCCATCGCtt > 1:1756071/1‑71 (MQ=255) aTAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCTATCGCttt > 1:1357169/1‑71 (MQ=255) aaCAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCTATCGCttt > 1:415709/1‑68 (MQ=255) aaCAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCCATCGCTTTTcc > 1:250965/1‑71 (MQ=255) cAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCCATCGCTTTTCCgg < 1:1197007/71‑1 (MQ=255) tCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCTATCGCTTTTCCGGtata > 1:969521/1‑70 (MQ=255) ggCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCTATCGCTTTTCCGGTATaca < 1:2251498/70‑1 (MQ=255) cTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCTATCGCTTTTCCgg < 1:740114/60‑1 (MQ=255) cATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCTATCGCTTTTCCGGTATACACAGGATc > 1:2439539/1‑70 (MQ=255) tttGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCTATCGCTTTTCCGGTATACACAGGATCAAg > 1:1014893/1‑69 (MQ=255) tttGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCTATCGCTTTTCCGGTATACACAGGATCAAg > 1:1375825/1‑69 (MQ=255) tttGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCCATc < 1:1946737/43‑1 (MQ=255) tttGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCCATc < 1:372575/43‑1 (MQ=255) tttGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCCATc < 1:193101/43‑1 (MQ=255) tttGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCCATc < 1:119692/43‑1 (MQ=255) tttGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCCATCGCTTTTCCGGta > 1:2138104/1‑55 (MQ=255) aGCGTTTCTGACTGATACCGTCAATCAGCCCCGCTATCGCTTTTCCGGTATACACAGGATCAAGCAGAATg > 1:1555249/1‑71 (MQ=255) tCTGACTGATACCGTCAATCAGCCCCGCTATCGCtt > 1:1100602/1‑36 (MQ=255) ctgaTACCGTCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCAAg > 1:568478/1‑53 (MQ=255) gaTACCGTCAATCAGCCCCGCTATCGCTTTTCCGGTATACACAGGATCAAGCAGAATGCCTTCCAGCcgcg > 1:2666159/1‑71 (MQ=255) gTCAATCAGCCCCGCTATCGCTTTTCCGGTATACACAGGATCAAGCAGAATGCCTTCCAGCCGCGCCAGc > 1:568653/1‑70 (MQ=255) aaTCAGCCCCGCTATCGCTTTTCCGGTATACACAGGATCAAGCAGAATGCCTTCCAGCCGCGCCAGCAAtt > 1:882928/1‑71 (MQ=255) aaTCAGCCCCGCTATCGCTTTTCCGGTATACACAGGATCAAGCAGAATGCCTTCCAGCCGCGCCAGCAAtt > 1:1176693/1‑71 (MQ=255) aTCAGCCCCGCTATCGCTTTTCCGGTATACACAGGATCAAGCAGAATGc < 1:1078919/49‑1 (MQ=255) aGCCCCGCTATCGCTTTTCCGGTATACACAGGATCAAGCAGAATGCCTTCCAGCCGCGCCAGCAATTTCAc > 1:1069674/1‑71 (MQ=255) cccGCCATCGCTTTTCCGGTATACACAGGATCAAGCAGAATGCCTTCCAg > 1:701880/1‑50 (MQ=255) cccGCCATCGCTTTTCCGGTATACACAGGATCAAGCAGAATGCCTTCCAg > 1:2681392/1‑50 (MQ=255) cccGCCATCGCTTTTCCGGTATACACAGGATCAAGCAGAATGCCTTCCAGCCGCGCCAGCAATTTCACTGc > 1:2367500/1‑71 (MQ=255) gCCATCGCTTTTCCGGTATACACAGGATCAAGCAGAATGCCTTCCAGCCGCGCCAGCAATTTCACTGCTTc < 1:2142410/71‑1 (MQ=255) | TGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGATCAAGCAGAATGCCTTCCAGCCGCGCCAGCAATTTCACTGCTTC > W3110S.gb/2000677‑2000810 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |