| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,002,183 | G→A | 15.2% | R114C (CGC→TGC) | fliY ← | cystine transporter subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,002,183 | 0 | G | A | 15.2% | 79.1 / 7.4 | 33 | R114C (CGC→TGC) | fliY | cystine transporter subunit |
| Reads supporting (aligned to +/- strand): ref base G (15/12); new base A (3/2); total (19/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.49e-01 | |||||||||||
ACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTCATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCAT > W3110S.gb/2002115‑2002249 | aCCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCt > 1:1469476/1‑71 (MQ=255) aGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTCATCAGAAATGGt > 1:2498389/1‑71 (MQ=255) aGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTCATCAGAAATGGt < 1:1005167/71‑1 (MQ=255) gcgcCTGAATACCAGAAATGGTGTACGGGGTTGAGAAGTCGTATTTTTTCTTGCGCTCATCAGAAATGGTg < 1:963999/71‑1 (MQ=255) gcgcCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTCATCAGAAATGGTg < 1:2420726/71‑1 (MQ=255) gcgcCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTCATCAGAAATGGTg < 1:16508/71‑1 (MQ=255) gcCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTCATCAGAAATGGTGAc > 1:2087256/1‑71 (MQ=255) tACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCACTCATCAGAAATGGTGACCTGAtt > 1:336634/1‑70 (MQ=255) ccAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTCATCAGAAATGGTGACCTGATTAAt > 1:1526362/1‑71 (MQ=255) ccAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTCATCAGAAATGGTGACCTGATTAAt > 1:1017448/1‑71 (MQ=255) aTGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTCATCAGAAATgg > 1:1013471/1‑52 (MQ=255) aTGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTCATCAGAAATGGTGACCt > 1:621013/1‑58 (MQ=255) aTGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTCATCAGAAATGGTGACCTg > 1:1758264/1‑59 (MQ=255) tACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTCATCAGAAATGGTGACCTGATTAATCACCACATCaa < 1:1459398/70‑1 (MQ=255) cGGGGTTGAGAAATCGTATTTTTTCTTGCGCTCATCAGAAATGGTGACCTGa > 1:1661577/1‑52 (MQ=255) cGGGGTTGAGAAATCGTATTTTTTCTTGCGCTCATCAGAAATGGTGACCTGa > 1:2742675/1‑52 (MQ=255) cGGGGTTGAGAAATCGTATTTTTTCTTGCGCTCATCAGAAATGGTGACCTGATTAATCACCACATCAATAc > 1:2523495/1‑71 (MQ=255) cGGGGTTGAGAAATCGTATTTTTTCTTGC‑CTCATCAGAAATGGTGACCTGATTAATCACCACATCAATACg > 1:941743/1‑71 (MQ=255) ttGAGAAATCGTATTTTTTCTTGCGCTCATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTa < 1:905968/71‑1 (MQ=255) ttGAGAAATCGTATTTTTTCTTGCGCTCATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTa < 1:1739272/71‑1 (MQ=255) ttGAGAAATCGTATTTTTTCTTGCACTCATCAGAAATGGTGACCTGAtt < 1:2147069/49‑1 (MQ=255) tGAGAAATCGTATTTTTTCTTGCGCTCATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTa < 1:2586069/70‑1 (MQ=255) gagaAATCGTATTTTTTCTTGCACTCATCAGAAAt > 1:1058327/1‑35 (MQ=255) agaAATCGTATTTTTTCTTGCGCTCATCAGAAATGGTGACCTGATTaa < 1:1538317/48‑1 (MQ=255) agaAATCGTATTTTTTCTTGCGCTCATCAGAAATGGTGACCTGATTaa < 1:1323004/48‑1 (MQ=255) aaaTCGTATTTTTTCTTGCGCTCATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTc > 1:286807/1‑71 (MQ=255) aaTCGTATTTTTTCTTGCGCTCATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTcc < 1:721321/71‑1 (MQ=255) cGTATTTTTTCTTGCGCTCATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAg > 1:1208123/1‑70 (MQ=255) gTATTTTTTCTTGCGCTCATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGc < 1:2397162/70‑1 (MQ=255) gTATTTTTTCTTGCGCTCATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGc > 1:851107/1‑70 (MQ=255) gTATTTTTTCTTGCACTCATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGc > 1:2482515/1‑70 (MQ=255) ttttttCTTGCACTCATCAGAAATGGTGACCTGATTAATCACCACATCaa < 1:1964040/50‑1 (MQ=255) ttttCTTGCGCTCATCAGAAATGGTGACCTGATTAATCACCACATCAATa > 1:1243142/1‑50 (MQ=255) gcgcTCATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCAt < 1:1774559/69‑1 (MQ=255) | ACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTCATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCAT > W3110S.gb/2002115‑2002249 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |