| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,039,900 | A→G | 32.6% | I130I (ATT→ATC) | yedV ← | predicted sensory kinase in two‑component regulatory system with YedW |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,039,900 | 0 | A | G | 32.6% | 51.0 / 41.2 | 46 | I130I (ATT→ATC) | yedV | predicted sensory kinase in two‑component regulatory system with YedW |
| Reads supporting (aligned to +/- strand): ref base A (13/18); new base G (5/10); total (18/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.49e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CATGTTATGTCTGGCTGAAGCCAATTTAGCCACAGTAACCGTTAATGATGGCGAAACTTCATCAATATTAATTCGTAAAGCATCTATCTCTGTATCATTAATAATGCTTCTGTAAATGCCAGCTGCGCTGATTGTCTC > W3110S.gb/2039831‑2039968 | cATGTTATGTCTGGCTGAAGCCAATTTAGCCACAGTAACCGTTAATGATGGCGAAACTTCATCAATATTaa > 1:473213/1‑71 (MQ=255) gCTGAAGCCAATTTAGCCACAGTAACCGTTAATGATGGCGAAACTTCATCAATATTGATTCGTAAAGc < 1:842920/68‑1 (MQ=255) gCTGAAGCCAATTTAGCCACAGTAACCGTTAATGATGGCGAAACTTCATCAATATTAATTCGTAAAGCatc > 1:2222836/1‑71 (MQ=255) cAATTTAGCCACAGTAACCGTTAATGATGGCGAAACTTCATCAATATTAATTCGTAAAGCATCTATCTCTg < 1:1640090/71‑1 (MQ=255) aaTTTAGCCACAGTAACCGTTAATGATGGCGAAACTTCATCAATATTaa > 1:2365456/1‑49 (MQ=255) aaTTTAGCCACAGTAACCGTTAATGATGGCGAAACTTCATCAATATTaa > 1:2679805/1‑49 (MQ=255) ccACAGTAACCGTTAATGATGGCGAAACTTCATCAATATTGATTCGTAAAGCATCTATCTCTGTATCAtt < 1:2318367/70‑1 (MQ=255) ccACAGTAACCGTTAATGATGGCGAAACTTCATCAATATTAATTCGTAAAGCa < 1:2764132/53‑1 (MQ=255) ccACAGTAACCGTTAATGATGGCGAAACTTCATCAATATTAATTCGTAAAGCa < 1:1650613/53‑1 (MQ=255) ccACAGTAACCGTTAATGATGGCGAAACTTCATCAATATTAATTCGTAAAGCATCTATCTCTGTATCATta < 1:584470/71‑1 (MQ=255) cacaGTAACCGTTAATGATGGCGAAACTTCATCAATATTAATTCGTAAAGCATCTATCTCTGTATCATtaa < 1:2589781/71‑1 (MQ=255) cacaGTAACCGTTAATGATGGCGAAACTTCATCAATATTAATTCGTAAAGCATCTATCTCTGTATCATtaa < 1:1509086/71‑1 (MQ=255) cacaGTAACCGTTAATGATGGCGAAACTTCAT‑AATATTGATTCGTAAAGCat > 1:2648048/1‑52 (MQ=255) acaGTAACCGTTAATGATGGCGAAACTTCATCAATATTGATTCGTAAAGCATCTATCTCTGTATCATtaat < 1:1108038/71‑1 (MQ=255) acaGTAACCGTTAATGATGGCGAAACTTCATCAATATTAATTCGTAAAGCATCTATCTCTGTATCATtaat < 1:575338/71‑1 (MQ=255) acaGTAACCGTTAATGATGGCGAAACTTCATCAATATTAATTCGTAAAGCATCTATCTCTGTATCATtaat < 1:1357892/71‑1 (MQ=255) caGTAACCGTTAATGATGGCGAAACTTCATCAATATTGATTCGTAAAGCatct < 1:36019/53‑1 (MQ=255) caGTAACCGTTAATGATGGCGAAACTTCATCAATATTGATTCGTAAAGCatct < 1:1769537/53‑1 (MQ=255) caGTAACCGTTAATGATGGCGAAACTTCATCAATATTAATTCGTAAAGCATCTATCTCTGTATCATtaata < 1:561366/71‑1 (MQ=255) gTAACCGTTAATGATGGCGAAACTTCATCAATATTGATTCGTAAAGCatcta > 1:1557512/1‑52 (MQ=255) gTAACCGTTAATGATGGCGAAACTTCATCAATATTGATTCGTAAAGCATCTATCTCTGTATCATTaataat < 1:569378/71‑1 (MQ=255) gTAACCGTTAATGATGGCGAAACTTCATCAATATTAATTCGTAAAGCATCTATCTCTGTATCATTaataat > 1:1014651/1‑71 (MQ=255) aCCGTTAATGATGGCGAAACTTCATCAATATTAATTCGTAAAGCATCTATCTCTGTATCATtaataa > 1:2255377/1‑67 (MQ=255) cGTTAATGATGGCGAAACTTCATCAATATTAATTCGTAAAGCatc < 1:194334/45‑1 (MQ=255) ttAATGATGGCGAAACTTCATCAATATTAATTCGTAAAGCatcta > 1:316618/1‑45 (MQ=255) aaTGATGGCGAAACTTCATCAATATTGATTCGTAAAGCATCTATCTCTGTATCATTAATAATGCTTCTg > 1:352644/1‑69 (MQ=255) atgatgGCGAAACTTCATCAATATTGATTCGTAAAGCATCTATCTCTGTATCATTAATAATGCTTCTGTa < 1:2664332/70‑1 (MQ=255) atgGCGAAACTTCATCAATATTAATTGGTAAAGCATCTATCTCTg > 1:26397/1‑45 (MQ=255) atgGCGAAACTTCATCAATATTAATTCGTAAAGCATCTATCTCTg > 1:192371/1‑45 (MQ=255) atgGCGAAACTTCATCAATATTAATTCGTAAAGCATCTATCTCTg > 1:2208892/1‑45 (MQ=255) gCGAAACTTCATCAATATTGATTCGTAAAGCatct < 1:277656/35‑1 (MQ=255) gCGAAACTTCATCAATATTAATTCGTAAAGCATCTATCTCTGTATCATTAATAATGCTTCTGTAAATGcc < 1:2289171/70‑1 (MQ=255) gCGAAACTTCATCAATATTAATTCGTAAAGCATCTATCTCTGTATCATTAATAATGCTTCTGTAAATGc > 1:2175354/1‑69 (MQ=255) aaCTTCATCAATATTAATTCGTAAAGCATCTATCTCTGTATCATtaat > 1:974390/1‑48 (MQ=255) aaCTTCATCAATATTAATTCGTAAAGCATCTATCTCTGTATCATTAATAATGCTTCTGTAAATGCCAGCTg > 1:760940/1‑71 (MQ=255) aCTTCATCAATATTGATTCGTAAAGCATCTATCTCTGTATCATTAATAATGCTTCTGTAAATGCCAGCTgc > 1:2513560/1‑71 (MQ=255) aCTTCATCAATATTGATTCGTAAAGCATCTATCTCTGTATCATTAATAATGCTTCTGTAAATGCCAGCTgc > 1:1961284/1‑71 (MQ=255) aCTTCATCAATATTAATTCGTAAAGCATCTATCTCTGTATCATTAATAATGCTTCTGTAAATGCCAGCTgc < 1:2546828/71‑1 (MQ=255) aCTTCATCAATATTAATTCGTAAAGCATCTATCTCTGTATCATTAATAATGCTTCTGTAAATGCCAGCTgc < 1:1668002/71‑1 (MQ=255) cTTCATCAATATTAATTCGTAAAGCATCTATCTCTGTATCATtaataa < 1:1800454/48‑1 (MQ=255) cTTCATCAATATTAATTCGTAAAGCATCTATCTCTGTATCATTaataat > 1:2674774/1‑49 (MQ=255) cTTCATCAATATTAATTCGTAAAGCATCTATCTCTGTATCATTAATAATg > 1:1057683/1‑50 (MQ=255) tcatcaATATTAATTCGTAAAGCATCTATCTCTGTa > 1:858251/1‑36 (MQ=255) atcaATATTGATTCGTAAAGCATCTATCTCTGTATCATTAATAATGCTTCTGTAAATGCCAGCTGCGCTg < 1:2164796/70‑1 (MQ=255) atcaATATTAATTCGTAAAGCATCTATCTCTGTATCATtaat < 1:461383/42‑1 (MQ=255) atcaATATTAATTCGTAAAGCATCTATCTCTGTATCATTAATAATGCTTCTGTAAATGCCAGCTGCGCTg < 1:468695/70‑1 (MQ=255) atcaATATTAATTCGTAAAGCATCTATCTCTGTATCATTAATAATGCTTCTGTAAATGCCAGCTGCGCTg < 1:1214586/70‑1 (MQ=255) tatTGATTCGTAAAGCATCTATCTCTGTATCATTAATAATGCTTCTGTAAATGCCAGCTGCGCTGAt < 1:1645130/67‑1 (MQ=255) ttAATTCGTAAAGCATCTATCTCTGTATCATTAATAATGCTTCTGTAAATGCCAGCTGCGCTGATTGtctc < 1:2031214/71‑1 (MQ=255) | CATGTTATGTCTGGCTGAAGCCAATTTAGCCACAGTAACCGTTAATGATGGCGAAACTTCATCAATATTAATTCGTAAAGCATCTATCTCTGTATCATTAATAATGCTTCTGTAAATGCCAGCTGCGCTGATTGTCTC > W3110S.gb/2039831‑2039968 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |