| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,041,745 | C→T | 100% | A44V (GCC→GTC) | yedY → | predicted reductase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,041,745 | 0 | C | T | 87.5% | 55.5 / 2.2 | 24 | A44V (GCC→GTC) | yedY | predicted reductase |
| Reads supporting (aligned to +/- strand): ref base C (0/3); new base T (11/10); total (11/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.23e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.53e-01 | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
GTGTTAAAAGCACTGGGCATCAGTGCAACTGCACTTTCTTTGCCTCACGCTGCGCATGCCGATCTGCTTAGCTGGTTTAAAGGGAACGATCGCCCG > W3110S.gb/2041687‑2041782 | gtgtTAAAAGCACTGGGCATCAGTGCAACTGCACTTTCTTTGCCTCACGCTGCGCATGTCGa < 1:827401/62‑1 (MQ=255)gtgtTAAAAGCACTGGGCATCAGTGCAACTGCACTTTCTTTGCCTCACGCTCCGCATGTCGa < 1:1668146/62‑1 (MQ=255) gtTAAAAGCACTGGGCATCAGTGCAACTGCACTTTCTTTGCCTCACGCTGCGCATGTCGATCTGCTTAGCt > 1:1668591/1‑71 (MQ=255) aaaaGCACTGGGCATCAGTGCAACTGCACTTTCTTTGCCTCACGCTGCGCATGTCGAt > 1:2093409/1‑58 (MQ=255) gCACTGGGCATCAGTGCAACTGCACTTTCTTTGCCTCACGCTGCGCATGTCGATCTGCTTAGCTGGTTTaa < 1:2215792/71‑1 (MQ=255) gCACTGGGCATCAGTGCAACTGCACTTTCTTTGCCTCACGCTGCGCATGTCGATCTGCTTAGCTGGTTTaa < 1:49985/71‑1 (MQ=255) tCAGTGCAACTGCACTTTCTTTGCCTCACGCTGCGCATGTCg < 1:1907312/42‑1 (MQ=255) tCAGTGCAACTGCACTTTCTTTGCCTCACGCTGCGCATGTCg < 1:28443/42‑1 (MQ=255) tCAGTGCAACTGCACTTTCTTTGCCTCACGCTGCGCATGTCGATCTGCTTAGCTGGTTTAAAGGGAACg < 1:595882/69‑1 (MQ=255) aGTGCAACTGCACTTTCTTTGCCTCACGCTGCGCATGTCGATCTGCTTAGCTGGTTTAAAGGGAACGAt > 1:1453630/1‑69 (MQ=255) aGTGCAACTGCACTTTCTTTGCCTCACGCTGCGCATGTCGATCTGCTTAGCTGGTTTAAAGGGAACGAt > 1:788721/1‑69 (MQ=255) aGTGCAACTGCACTTTCTTTGCCTCACGCTGCGCATGTCGATCTGCTTAGCTGGTTTAAAGGGAACGAt > 1:1974001/1‑69 (MQ=255) aGTGCAACTGCACTTTCTTTGCCTCACGCTGCGCATGTCGATCTGCTTAGCTGGTTTAAAGGGAACGAt > 1:2243711/1‑69 (MQ=255) aGTGCAACTGCACTTTCTTTGCCTCACGCTGCGCATGTCGATCTGCTTAGCTGGTTTAAAGGGAACGAt > 1:1406871/1‑69 (MQ=255) aGTGCAACTGCACTTTCTTTGCCTCACGCTGCGCATGTCGATCTGCTTAGCTGGTTTAAAGGGAACGATcg > 1:2416091/1‑71 (MQ=255) tGCAACTGCACTTTCTTTGCCTCACGCTGCGCATGTCGATCTGCTTAGc < 1:414998/49‑1 (MQ=255) gCAACTGCACTTTCTTTGCCTCACGCTGCGCATGTCGATCTGCTTAGCTGGTTTAAAGGGaa > 1:920179/1‑62 (MQ=255) gCAACTGCACTTTCTTTGCCTCACGCTGCGCATGTCGATCTGCTTAGCTGGTTTAAAGGGAACGATcgccc > 1:2532832/1‑71 (MQ=255) gCAACTGCACTTTCTTTGCCTCACGCTGCGCATGTCGATCTGCTTAGCTGGTTTAAAGGGAACGATcgccc > 1:229452/1‑71 (MQ=255) cAACTGCACTTTCTTTGCCTCACGCTGCGCATGTCGATCTGCTTAGCTGGTTTAAAGGGAACGATcgcccg < 1:977557/71‑1 (MQ=255) aCTGCACTTTCTTTGCCTCACGCTGCGCATGCCGATCTGCTTAGCTGGTTTAAAGGGAACg < 1:1943011/61‑1 (MQ=255) aCTGCACTTTCTTTGCCTCACGCTGCGCATGCCGATCTGCTTAGCTGGTTTAAAGGGAACg < 1:1688016/61‑1 (MQ=255) tttctttGCCTCACGCTGCGCATGTCGATCTGCTTAGCTGGTTTAAAGGGAACg < 1:244318/54‑1 (MQ=255) aCGCTGCGCATGCCGATCTGCTTAGCTGGTTTAAAggg < 1:370877/38‑1 (MQ=255) | GTGTTAAAAGCACTGGGCATCAGTGCAACTGCACTTTCTTTGCCTCACGCTGCGCATGCCGATCTGCTTAGCTGGTTTAAAGGGAACGATCGCCCG > W3110S.gb/2041687‑2041782 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |