| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,065,027 | G→T | 55.8% | A145D (GCT→GAT) | erfK ← | conserved hypothetical protein with NAD(P)‑binding Rossmann‑fold domain |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,065,027 | 0 | G | T | 55.8% | 4.7 / 55.1 | 34 | A145D (GCT→GAT) | erfK | conserved hypothetical protein with NAD(P)‑binding Rossmann‑fold domain |
| Reads supporting (aligned to +/- strand): ref base G (11/4); new base T (11/8); total (22/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.76e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ATGCGGGCAAACTCTCCCCTCGTTTCGCATATTCGCGCCGAGTGTTCGGCGTTGGCGTCCAGGTTGGAGCTTCTTGTTTACGTTCAACGGTAGTCACCCAGTTACGCGGGGTTTCTCGCCCAGCCTGGCCGA > W3110S.gb/2064959‑2065090 | aTGCGGGCAAACTCTCCCCTCGTTTCGCATATTCGCGCCGAGTGTTCGGCGTTGGCGTCCAGGTTGGATct > 1:1688612/1‑71 (MQ=255) cGGGCAAACTCTCCCCTCGTTTCGCATATTCGCGCCGAGTGTTCGGCGTTGGCGTCCAGGTTGGAGcttct > 1:1435113/1‑71 (MQ=255) cAAACTCTCCCCTCGTTTCGCATATTCGCGCCGAGTGTTCGGCGTTGGCGTCCAGGTTGGATCTTCTTGtt < 1:2056364/71‑1 (MQ=255) ctctCCCCTCGTTTCGCATATTCGCGCCGAGTGTTCGGCGTTGGCGTCCAGGTTGGATCTTCTTGTTTACg > 1:1260488/1‑71 (MQ=255) gTTTCGCATATTCGCGCCGAGTGTTCGGCGTTGGCGTCCAGGTTGGAGCTTCTTGTTTACGTTCAACGGTa < 1:2260497/71‑1 (MQ=255) cATATTCGCGCCGAGTGTTCGGCGTTGGCGTCCAGGTTGGAGCTTCTTGTTTACGTTCAACGGTAGTCAcc > 1:89440/1‑71 (MQ=255) atatTCGCGCCGAGTGTTCGGCGTTGGCGTCCAGGTTGGATCTTCTTGtt > 1:1205001/1‑50 (MQ=255) tatTCGCGCCGAGTGTTCGGCGTTGGCGTCCAGGTTGGATCTTCTTGTTTACGTTCAACGGTAGTCAcc > 1:2131135/1‑69 (MQ=255) tatTCGCGCCGAGTGTTCGGCGTTGGCGTCCAGGTTGGATCTTCTTGTTTACGTTCAACGGTAGTCACCCa < 1:1116244/71‑1 (MQ=255) tatTCGCGCCGAGTGTTCGGCGTTGGCGTCCAGGTTGGATCTTCTTGTTTACGTTCAACGGTAGTCACCCa < 1:1523685/71‑1 (MQ=255) ttCGCGCCGAGTGTTCGGCGTTGGCGTCCAGGTTTGAGCTTCTTTTTTACGTTCAACGGTAGTCACCCAGt < 1:1399530/71‑1 (MQ=255) ttCGCGCCGAGTGTTCGGCGTTGGCGTCCAGGTTGGATCTTCTTGTTTACGTTCAACGGTAGTCACCCAGt > 1:1921515/1‑71 (MQ=255) ttCGCGCCGAGTGTTCGGCGTTGGCGTCCAGGTTGGATCTTCTTGTTTACGTTCAACGGTAGTCACCCAGt > 1:575084/1‑71 (MQ=255) ttCGCGCCGAGTGTTCGGCGTTGGCGTCCAGGTTGGAGCTTCTTGTTTACGTTCAACGGTAGTCACCCatt < 1:2609776/71‑3 (MQ=255) ttCGCGCCGAGTGTTCGGCGTTGGCGTCCAGGTTGGAGCTTCTTGTTTACGTTCAACGGTAGTCACCCAGt > 1:1675479/1‑71 (MQ=255) tCGCGCCGAGTGTTCGGCGTTGGCGTCCAGGTTGGAGCTTCTTGt > 1:2553646/1‑45 (MQ=255) tCGCGCCGAGTGTTCGGCGTTGGCGTCCAGGTTGGAGCTTCTTGTTTACGTTCAACGGTAGTCACCCAGt > 1:2595201/1‑70 (MQ=255) tCGCGCCGAGTGTTCGGCGTTGGCGTCCAGGTTGGAGCTTCTTGTTTACGTTCAACGGTAGTCACCCAGt > 1:2057050/1‑70 (MQ=255) cgcgCCGAGTGTTCGGCGTTGGCGTCCAGGTTGGATCTTCTTGtt > 1:2724063/1‑45 (MQ=255) cgcgCCGAGTGTTCGGCGTTGGCGTCCAGGTTGGATCTTCTTGTTTACGTTCAACGGTa > 1:378845/1‑59 (MQ=255) ttCGGCTTTGGCGTCCAGGTTGGATCTTCTTGTTTACGTTCa < 1:2777780/42‑1 (MQ=38) ttCGGCGTTGGCGTCCAGGTTGGAGCTTCTTGTTTACGTTCAACGGTAGTCACCCAGTTACGCGGGGttt > 1:1286051/1‑70 (MQ=255) ttCGGCGTTGGCGTCCAGGTTGGAGCTTCTTGTTTACGTTCAACGGTAGTCACCCAGTTACGCGGGGTTtc > 1:704697/1‑71 (MQ=255) cGGCGTTGGCGTCCAGGTTGGATCTTCTTGTTTACGTTCAAc > 1:1811649/1‑42 (MQ=255) cGGCGTTGGCGTCCAGGTTGGAGCTTCTTGTTTACGTTCAACGGTAGTCACCCAGTTACGCGGGGTTtctc > 1:998018/1‑71 (MQ=255) ggCGTTGGCGTCCAGGTTGGATCTTCTTGTTTACGTTCAACGGTAGTCACCCAGTTACGCGGGGTTTCTCg < 1:992564/71‑1 (MQ=255) ttGGCGTCCAGGTTGGATCTTCTTGTTTACGTTCAACGGTAGTCACCCAGTTACGCGGGGTTTCTCGCCCa < 1:2241088/71‑1 (MQ=255) ggCGTCCAGGTTGGAGCTTCTTGTTTACGTTCAACGGTAGTCa > 1:1941184/1‑43 (MQ=255) gCGTCCAGGTTGGATCTTCTTGTTTACGTTCAACGGTAGTCACCCAGTTACGCGGGGTTTCTCGCCCAGc < 1:1193927/70‑1 (MQ=255) tCCAGGTTGGATCTTCTTGTTTACGTTCAACGGTAGTCACCCAGTTACGCGGGGTTtctc > 1:1787689/1‑60 (MQ=255) ttGGATCTTCTTGTTTACGTTCAACGGTAGTCACCCAGTTACGCGGGGttt < 1:1738910/51‑1 (MQ=255) ttGGATCTTCTTGTTTACGTTCAACGGTAGTCACCCAGTTACGCGGGGTTTCTCGCCCAGCCTGGCCGa > 1:656277/1‑69 (MQ=255) ggAGCTTCTTGTTTACGTTCAACGGTAGTCACCCAGTTACGCGGGGt > 1:1021241/1‑47 (MQ=255) gAGCTTCTTGTTTACGTTCAACGGTAGTCACCCAGTTAcgcg < 1:7028/42‑1 (MQ=255) | ATGCGGGCAAACTCTCCCCTCGTTTCGCATATTCGCGCCGAGTGTTCGGCGTTGGCGTCCAGGTTGGAGCTTCTTGTTTACGTTCAACGGTAGTCACCCAGTTACGCGGGGTTTCTCGCCCAGCCTGGCCGA > W3110S.gb/2064959‑2065090 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |